8 isoprostane enzyme immunoassay kit

To assess the degree of lipid peroxidation and oxidative stress in damaged livers, we measured 8-isoprostane levels in the hepatic tissue using the 8-Isoprostane enzyme immunoassay kit (Cayman Chemical, Ann Arbor, MI). The assay was carried out as previously described.^{(14 (link))}

To assay F2-isoP, acute phase and 48-hour plasma samples were frozen at -80 degrees Celsius prior to processing. F2-isoP was quantified using an 8-Isoprostane Enzyme Immunoassay Kit (Cayman Chemical, Ann Arbor, MI) by the Antioxidants Research Laboratory at the Jean Mayer USDA Human Nutrition Research Center on Aging (HNRCA) at Tufts University.

Blood samples were collected at admission, less than 9 hours after stroke onset. To avoid delays in treatment, all blood samples from patients eligible for IV thrombolytic treatment were obtained after the treatment was initiated. Samples were analyzed by investigators blinded to clinical information.
Oxidative stress biomarkers F2-isoP, urinary 8-OHdG, and plasma ORAC assay were evaluated in this study (total ORAC [ORACTOTAL] and perchloric acid ORAC [ORACPCA]). Inflammatory and tissue damage biomarkers high sensitivity C reactive protein (hs-CRP), matrix metalloproteinase (MMP) 2 and 9 were also evaluated.
For the quantification of F2-isoP, blood samples were collected in 10 mL ethylenediaminetetraacetic acid (EDTA) tubes. Plasma was frozen at −80°C prior to processing. F2-isoP was quantified using an 8-Isoprostane Enzyme Immunoassay Kit (Cayman Chemical, Ann Arbor, MI). Laboratory methods used for the quantification of the other biomarkers are reported in the supplemental material.