Anti ppkm2

Manufactured by Cell Signaling Technology

Anti-pPKM2 is a primary antibody that detects the phosphorylated form of the enzyme pyruvate kinase M2 (PKM2). PKM2 is a key regulator of cellular metabolism and is involved in the Warburg effect, a metabolic shift observed in cancer cells.

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Lab products found in correlation

Anti pkm2, by Cell Signaling Technology (4 mentions) Ecl chemiluminescence kit, by Thermo Fisher Scientific (2 mentions) Anti grp78 bip, by Cell Signaling Technology (2 mentions) Anti β actin, by Cell Signaling Technology (2 mentions) Anti chop, by Cell Signaling Technology (2 mentions) Anti lc3b 1 2, by Cell Signaling Technology (2 mentions) Trypsin edta, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Ab11672, by Abcam (1 mentions) Anti hk2, by Cell Signaling Technology (1 mentions) Anti sumo1, by Abcam (1 mentions) Anti β actin a5441, by Merck Group (1 mentions) Anti stat5a, by Abcam (1 mentions) Il 17α, by R&D Systems (1 mentions) Anti sae1, by Abcam (1 mentions) Recombinant human tnf α, by R&D Systems (1 mentions) Anti pfkp, by Cell Signaling Technology (1 mentions)

4 protocols using anti ppkm2

Western Blot Analysis of Cellular Signaling

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Cell lysates were standardized for protein content, resolved on 4% to 12% SDS polyacrylamide gels, and blotted onto nitrocellulose membranes. Membranes were probed with rabbit anti-pPKM2, anti-PKM2, anti-CHOP, anti-GRP78/BIP, anti-pEGFR (Y1086), anti-EGFR, anti-LC3B-I/II, and anti-β-actin (Cell Signaling). Antibody binding was detected by using an ECL Chemiluminescence Kit (Thermo Scientific).

Sobhakumari A., Orcutt K.P., Love-Homan L., Kowalski C.E., Parsons A.D., Knudson C.M, & Simons A.L. (2016). 2-Deoxy-d-glucose Suppresses the In Vivo Antitumor Efficacy of Erlotinib in Head and Neck Squamous Cell Carcinoma Cells. Oncology Research, 24(1), 55-64.

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Western Blot Analysis of Cellular Stress

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Cell lysates were standardized for protein content, resolved on 4% to 12% SDS polyacrylamide gels, and blotted onto nitrocellulose membranes. Membranes were probed with rabbit anti-pPKM2, anti-PKM2, anti-CHOP, anti-GRP78/BIP, anti-pEGFR (Y1086), anti-EGFR, anti-LC3B-I/II and anti-β-actin (Cell Signaling). Antibody binding was detected by using an ECL Chemiluminescence Kit (Thermo Scientific).

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Investigating SUMO Regulation in Metabolic Pathways

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Recombinant human TNF-α, IL-1β, and IL-17α were purchased from R&D Systems (Bio-Techne), and LPS was obtained from MilliporeSigma. DMEM, FBS, antibiotics, trypsin EDTA, PBS, and other products for cell culture were purchased from Invitrogen, Thermo Fisher Scientific. The primary antibodies used were as follows: anti-SAE1 (ab38434, Abcam), anti-UBA2 (ab189289, Abcam), anti–SUMO-1 (ab11672, Abcam), and anti-STAT5A (ab32043, Abcam) were purchased from Abcam. Anti-HK2 (2867, Cell Signaling Technology), anti-PFKP (8164, Cell Signaling Technology), anti-PKM2 (4053, Cell Signaling Technology), and anti–p-PKM2 (3827, Cell Signaling Technology) were purchased from Cell Signaling Technology. Anti–β-actin (A5441) was purchased from MilliporeSigma. GA (15:1) was purchased from MilliporeSigma. SKN was purchased from Selleckchem.

Wang C., Xiao Y., Lao M., Wang J., Xu S., Li R., Xu X., Kuang Y., Shi M., Zou Y., Wang Q., Liang L., Zheng S.G, & Xu H. (2020). Increased SUMO-activating enzyme SAE1/UBA2 promotes glycolysis and pathogenic behavior of rheumatoid fibroblast-like synoviocytes. JCI Insight, 5(18), e135935.

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Immunohistochemical Analysis of Tumor Markers

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Tumor tissues embedded in paraffin were subjected to hematoxylin and eosin (H&E) staining and immunohistochemistry (IHC). Tissue samples were dewaxed and rehydrated, submerged into EDTA antigenic retrieval buffer, and treated with 3% hydrogen peroxide. Slides were incubated with the following primary antibodies overnight at 4 °C and with secondary antibodies (Enzyme-conjugated goat anti-rabbit or anti-mouse) for 60 minutes at 37 °C. 3,30-Diaminobenzidine was used to visualize the target proteins and then counterstained with hematoxylin. After staining, the sections were photographed and analyzed using the ImageJ software (1.8.0, USA). Primary antibodies were as follows: anti‐PKM2 (1:800, 4053S; Cell Signaling Technology, Beverly, MA), anti‐p-PKM2 (1:400, 3827S; Cell Signaling Technology, Beverly, MA), anti‐p-STAT3 (Y705, 1:200, 9145S; Cell Signaling Technology, Beverly, MA), anti‐GLUT1 (1:400, 66290‐1‐Ig; Proteintech) and anti-HK2 (1:300, 22029‐1‐AP; Proteintech).

Zhang Q., Liu Q., Zheng S., Liu T., Yang L., Han X, & Lu X. (2021). Shikonin Inhibits Tumor Growth of ESCC by suppressing PKM2 mediated Aerobic Glycolysis and STAT3 Phosphorylation. Journal of Cancer, 12(16), 4830-4840.

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