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Penicillin streptomycin mixture ps

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Penicillin-streptomycin mixture (PS) is a commonly used antibiotic solution for cell culture. It contains a combination of the antibiotics penicillin and streptomycin, which inhibit the growth of a wide range of bacteria.

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Lab products found in correlation

Ldn193189, by Fujifilm (3 mentions) Sb431542, by Fujifilm (3 mentions) 2 mercaptoethanol 2 me, by Merck Group (3 mentions) U0126, by Enzo Life Sciences (2 mentions) Y 27632, by Fujifilm (2 mentions) Carbonyl cyanide m chlorophenylhydrazone cccp, by Merck Group (1 mentions) Poly l ornithine, by Merck Group (1 mentions) Remdesivir, by Selleck Chemicals (1 mentions) Prmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Rosiglitazone rgz, by Merck Group (1 mentions) Chir99021, by Fujifilm (1 mentions) Dimethyl sulfoxide (dmso), by Fujifilm (1 mentions)

5 protocols using penicillin streptomycin mixture ps

1

Investigating Molecular Mechanisms in Cellular Processes

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Chlorpyrifos (CPF), Y-27632, SB431542, and LDN193189 were obtained from Wako (Tokyo, Japan). Penicillin-streptomycin mixture (PS) was obtained from Thermo Fisher Scientific (Waltham, MA, USA). U0126 was obtained from Enzo Life Sciences (Farmingdale, NY, USA). Poly-L-ornithine, 2-mercaptoethanol (2-ME), and carbonylcyanide m-chlorophenylhydrazone (CCCP) were obtained from Sigma-Aldrich (St. Louis, MO, USA). All other reagents were of analytical grade and obtained from commercial sources.
Yamada S., Kubo Y., Yamazaki D., Sekino Y, & Kanda Y. (2017). Chlorpyrifos inhibits neural induction via Mfn1-mediated mitochondrial dysfunction in human induced pluripotent stem cells. Scientific Reports, 7, 40925.
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2

Remdesivir and Cell Culture Protocol

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Remdesivir was obtained from Selleck Chemicals (Houston, TX). Penicillin-streptomycin mixture (PS) was obtained from Thermo Fisher Scientific (Waltham, MA, USA). All other reagents were of analytical grade and were obtained from commercial sources.
Yamada S., Noda T., Okabe K., Yanagida S., Nishida M, & Kanda Y. (2022). SARS-CoV-2 induces barrier damage and inflammatory responses in the human iPSC-derived intestinal epithelium. Journal of Pharmacological Sciences, 149(3), 139-146.
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3

Cell Culture of HEK293T and A549

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HEK293T and stably expressing Flag-OGT1 HEK293FRT cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Life Technologies, Waltham, MA, USA), and lung carcinoma type II epithelium-like A549 cells were cultured in PRMI 1640 medium (Gibco, Life Technologies, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS; KangYuan Biology, Beijing, China) and 1% penicillin-streptomycin mixture (P/S; Thermo Fisher Scientific, Waltham, MA, USA) at 37 °C in the presence of 5% CO2.
Zhao L., Li M., Wei T., Feng C., Wu T., Shah J.A., Liu H., Wang F., Cai Y, & Jin J. (2019). O-GlcNAc-Modification of NSL3 at Thr755 Site Maintains the Holoenzyme Activity of MOF/NSL Histone Acetyltransferase Complex. International Journal of Molecular Sciences, 21(1), 173.
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4

Stem Cell Differentiation Reagents

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TBT was obtained from Tokyo Chemical Industry (Tokyo, Japan) and dissolved in dimethyl sulfoxide (DMSO). Rosiglitazone (RGZ), 2-mercaptoethanol (2-ME), and sodium butyrate (NaB) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Y-27632, SB431542, LDN193189, and CHIR99021 were obtained from Wako (Tokyo, Japan). The penicillin-streptomycin mixture (PS) was obtained from Thermo Fisher Scientific (Waltham, MA, USA). U0126 was obtained from Enzo Life Sciences (Farmingdale, NY, USA). All other reagents were of analytical grade and were obtained from commercial sources.
Yamada S., Kubo Y., Yamazaki D., Sekino Y., Nomura Y., Yoshida S, & Kanda Y. (2018). Tributyltin Inhibits Neural Induction of Human Induced Pluripotent Stem Cells. Scientific Reports, 8, 12155.
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5

Reagents for Cell Culture Protocol

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5-FU was obtained from Enzo Life Sciences (Farmingdale, NY, USA). 2-mercaptoethanol (2-ME) was obtained from Sigma-Aldrich (St. Louis, MO, USA). Dimethylsulfoxide (DMSO), Y-27632, SB431542, and LDN193189 were obtained from Wako (Tokyo, Japan). Penicillin-streptomycin mixture (PS) was obtained from Thermo Fisher Scientific (Waltham, MA, USA). All other reagents were of analytical grade and obtained from commercial sources.
Yamada S., Yamazaki D, & Kanda Y. (2018). 5-Fluorouracil inhibits neural differentiation via Mfn1/2 reduction in human induced pluripotent stem cells. The Journal of toxicological sciences, 43(12).
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