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Bovine growth serum (bgs)

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Bovine growth serum is a cell culture supplement derived from the serum of bovine (cattle) blood. It is commonly used to support the growth and proliferation of cells in various cell culture applications.

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Lab products found in correlation

Penicillin streptomycin, by Thermo Fisher Scientific (7 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (5 mentions) Trypsin edta, by Thermo Fisher Scientific (3 mentions) Mycoalert, by Lonza (2 mentions) Penicillin streptomycin, by Corning (2 mentions) Dulbecco modified eagle medium (dmem), by Merck Group (2 mentions) Penicillin streptomycin glutamine (psg), by Thermo Fisher Scientific (2 mentions) L glutamine, by Thermo Fisher Scientific (2 mentions) Glutamax, by Thermo Fisher Scientific (2 mentions) Sw480, by American Type Culture Collection (2 mentions) Sw620, by American Type Culture Collection (2 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions) Rpmi 1640, by Thermo Fisher Scientific (2 mentions) M199 medium, by Thermo Fisher Scientific (1 mentions) Pancreatin, by Merck Group (1 mentions) Collagenase, by Worthington (1 mentions) High glucose dmem, by Thermo Fisher Scientific (1 mentions) Fungizone, by Thermo Fisher Scientific (1 mentions) Lsm780 inverted, by Zeiss (1 mentions)

Close spelling variants of this product

Bovine growth serum (BGS, Fetalgro® Bovine Growth Serum (BGS Bovine serum

29 protocols using bovine growth serum (bgs)

1

Immortalized and Primary DM1 Myoblasts

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LHCN-M2 immortalized human satellite cells,72 (link) carrying 2 (CTG·CAG)5 alleles (5/5 in short), were grown on 0.1% (w/v) gelatin-coated plastic surfaces in skeletal muscle cell basal medium (PromoCell) with Supplement Mix (0.05 mL/mL fetal calf serum, 50 µg/mL fetuin (bovine), 10 ng/mL epidermal growth factor (recombinant human), 1 ng/mL basic fibroblast growth factor (recombinant human), 10 µg/mL insulin (recombinant human), 0.4 µg/mL dexamethasone), supplemented with 1% (v/v) GlutaMAX and 15% (v/v) bovine growth serum (Thermo Scientific) at 7.5% CO2 and 37°C.
Primary DM1 myoblasts (13/800),73 (link) were grown on 0.1% (w/v) gelatin-coated plastic surfaces in Ham's F10 medium (Gibco) supplemented with GlutaMAX and 20% (v/v) bovine growth serum (Thermo Scientific) at 7.5% CO2 and 37°C.
Immortalized DM500 mouse myoblasts expressing a human DM1 genomic fragment carrying a (CTG·CAG)n repeat of approximately 500 triplets74 (link) were grown on 0.1% (w/v) gelatin-coated plastic surfaces in proliferation medium containing DMEM (Gibco) supplemented with 20% (v/v) fetal bovine serum (PAA, Pasching, Austria), 4 mM L-glutamine (Gibco), 1 mM pyruvate (Sigma), 50 µg/mL gentamicin (Gibco), 20 units/mL γ-interferon (BD Biosciences) and 2% (v/v) chicken embryo extract (Sera Laboratories International) at 7.5% CO2 and 33°C.
Gudde A.E., van Heeringen S.J., de Oude A.I., van Kessel I.D., Estabrook J., Wang E.T., Wieringa B, & Wansink D.G. (2017). Antisense transcription of the myotonic dystrophy locus yields low-abundant RNAs with and without (CAG)n repeat. RNA Biology, 14(10), 1374-1388.
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2

Cell culture protocol for NIH 3T3, A549, and THP-1

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NIH 3T3 cells and A549 cells were purchased from ATCC and cultured in DMEM (Gibco) supplemented with 10% bovine growth serum (Gibco) and 1% penicillin/streptomycin (Gibco). NIH 3T3 cells between 6 and 12 passages were used in this study. A549 cells between 4 and 10 passages were used in this study. THP‐1 cells were purchased from ATCC. The cells were cultured in RPMI 1640 (Gibco) medium supplemented with 10% heated‐inactivated bovine growth serum and 1% penicillin/streptomycin (Gibco). THP‐1 cells between 10 and 15 passages were used in this study. All cells were cultured at 37 °C with 5% CO2 and passaged twice a week according to the standard protocols.
Hou Y., Jing J., Luo Y., Xu F., Xie W., Ma L., Xia X., Wei Q., Lin Y., Li K.H, & Chu Z. (2022). A Versatile, Incubator‐Compatible, Monolithic GaN Photonic Chipscope for Label‐Free Monitoring of Live Cell Activities. Advanced Science, 9(17), 2200910.
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3

Culturing and Maintaining Cell Lines for KSHV Research

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HEK293T (293T) cells were obtained from the American Type Culture Collection (Manassas, VA). SLK cells were obtained from the NIH AIDS Reagent Program. SLK and 293T cells were maintained at 37°C in Dulbecco's modified Eagle's medium (DMEM; Gibco-BRL) supplemented with 10% (vol/vol) bovine growth serum (BGS) (HyClone) and 15 μg/ml gentamicin. BJAB and BCBL-1 cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% (vol/vol) BGS and 15 μg/ml gentamicin. iSLK.219 cells are latently infected with a recombinant KSHV.219 (rKSHV.219) and carry integrated, doxycycline-inducible, KSHV replication transcription activator (RTA), and were maintained at 37°C in DMEM (Gibco) supplemented with 10% (vol/vol) BGS and 15 μg/ml gentamicin. In iSLK.219 cells, 10μg/ml puromycin in combination with 1 mg/ml G418 was used to maintain rKSHV.219 and the rtTA Tet-On transactivator, respectively. Human Dermal Microvascular Endothelial Cells (HDMVEC) (ATCC) were maintained at 37°C in Vascular Cell Basal Medium (ATCC PCS-100–030) supplemented with Microvascular Endothelial Cell Growth Kit-BBE (ATCC PCS-110-040) and 15 μg/ml gentamicin.
Li S., Liu B., Tan M., Juillard F., Szymula A., Álvarez Á.L., Van Sciver N., George A., Ramachandran A., Raina K., Tumuluri V.S., Costa C.N., Simas J.P, & Kaye K.M. (2024). Kaposi's sarcoma herpesvirus exploits the DNA damage response to circularize its genome. Nucleic Acids Research, 52(4), 1814-1829.
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4

Immortalized Fibroblast Cell Lines from COA6, SCO1, and SCO2 Patients

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Primary fibroblasts from control, COA6 (Baertling et al., 2015 (link)), SCO1–1 (R149X/P174L; Valnot et al., 2000 (link)), SCO1–2 (V93X/M294V; Leary et al., 2013 (link)) and SCO2–9 (Leary et al., 2013 (link)) were immortalized as described (Leary et al., 2004 (link)). Fibroblasts were transduced at 40%–60% confluency with retrovirus produced by the Phoenix amphotrophic packaging cell line, and selected in media containing hygromycin or puromycin to yield stable overexpression cell lines (Leary et al., 2004 (link)). Both fibroblasts and the packaging cells were grown in high-glucose DMEM containing 10% bovine growth serum (ThermoFisher) at 37°C in 5% CO2 and were tested to ensure that they were Mycoplasma-free (Lonza MycoAlert) before harvesting.
Soma S., Morgada M.N., Naik M.T., Boulet A., Roesler A.A., Dziuba N., Ghosh A., Yu Q., Lindahl P.A., Ames J.B., Leary S.C., Vila A.J, & Gohil V.M. (2019). COA6 Is Structurally Tuned to Function as a Thiol-Disulfide Oxidoreductase in Copper Delivery to Mitochondrial Cytochrome c Oxidase. Cell reports, 29(12), 4114-4126.e5.
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5

Isolation of Primary Neonatal Rat Ventricular Cardiomyocytes

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Primary neonatal rat ventricular cardiomyocytes (NRVMs) were isolated from 1-day to 2-day old Sprague-Dawley rat pups (Envigo, #002 timed mated), cultured on gelatin-coated dishes19 (link),64 (link), and maintained in M199 medium (ThermoFisher Scientific, #SH30253FS) supplemented with 2% bovine growth serum (ThermoFisher Scientific, #SH3054103) and 1× penicillin-streptomycin (Cellgro, #30-0002-CI) at 37 °C in 5% CO2. For the isolation procedure, neonatal hearts were collected, the atria were removed, and the ventricles were cut up in HBSS prior to enzymatic digestion. The ventricular tissue was subjected to 5 rounds of enzymatic digestion using 0.05% pancreatin (Sigma) and 84 units/ml of collagenase (Worthington, Lakewood, NJ). Cells were collected by centrifugation at 500×g for 5 min at 4 °C and resuspended in M199 medium. The cells were then differentially plated for 1 h on culture dishes to reduce fibroblasts. The cardiomyocytes were then plated on gelatinized cell culture dishes.
Schips T.G., Vanhoutte D., Vo A., Correll R.N., Brody M.J., Khalil H., Karch J., Tjondrokoesoemo A., Sargent M.A., Maillet M., Ross R.S, & Molkentin J.D. (2019). Thrombospondin-3 augments injury-induced cardiomyopathy by intracellular integrin inhibition and sarcolemmal instability. Nature Communications, 10, 76.
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6

COS-7 Cell Culture Protocol

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COS-7 cells (ATCC, #CRL-1651) were maintained in DMEM/high glucose (ThermoFisher Scientific, #SH30022.01) supplemented with 10% bovine growth serum (ThermoFisher Scientific, #SH3054103) and 1× penicillin-streptomycin (Cellgro, #30-0002-CI) at 37 °C in 5% CO2.
Schips T.G., Vanhoutte D., Vo A., Correll R.N., Brody M.J., Khalil H., Karch J., Tjondrokoesoemo A., Sargent M.A., Maillet M., Ross R.S, & Molkentin J.D. (2019). Thrombospondin-3 augments injury-induced cardiomyopathy by intracellular integrin inhibition and sarcolemmal instability. Nature Communications, 10, 76.
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7

Culturing HEK293 Cells for Research

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Experimental model for this study was derived from HEK293, a cell line exhibiting epithelial morphology that was isolated from the kidney of a human embryo. Cells were cultured in DMEM (PAN Biotech). DMEM were supplemented with 10% bovine growth serum (Thermo Fisher) and penicillin-streptomycin-glutamine (Gibco). Cells were grown at 37°C in standard cell culture incubators. All cells were routinely tested and confirmed to be free of mycoplasma contamination. A full list of cell lines used in the paper is included in the key resources table.
Polenkowski M., Allister A.B., Burbano de Lara S., Pierce A., Geary B., El Bounkari O., Wiehlmann L., Hoffmann A., Whetton A.D., Tamura T, & Tran D.D. (2022). THOC5 complexes with DDX5, DDX17, and CDK12 to regulate R loop structures and transcription elongation rate. iScience, 26(1), 105784.
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8

Immortalized Fibroblast Cell Lines from COA6, SCO1, and SCO2 Patients

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Primary fibroblasts from control, COA6 (Baertling et al., 2015 (link)), SCO1–1 (R149X/P174L; Valnot et al., 2000 (link)), SCO1–2 (V93X/M294V; Leary et al., 2013 (link)) and SCO2–9 (Leary et al., 2013 (link)) were immortalized as described (Leary et al., 2004 (link)). Fibroblasts were transduced at 40%–60% confluency with retrovirus produced by the Phoenix amphotrophic packaging cell line, and selected in media containing hygromycin or puromycin to yield stable overexpression cell lines (Leary et al., 2004 (link)). Both fibroblasts and the packaging cells were grown in high-glucose DMEM containing 10% bovine growth serum (ThermoFisher) at 37°C in 5% CO2 and were tested to ensure that they were Mycoplasma-free (Lonza MycoAlert) before harvesting.
Soma S., Morgada M.N., Naik M.T., Boulet A., Roesler A.A., Dziuba N., Ghosh A., Yu Q., Lindahl P.A., Ames J.B., Leary S.C., Vila A.J, & Gohil V.M. (2019). COA6 Is Structurally Tuned to Function as a Thiol-Disulfide Oxidoreductase in Copper Delivery to Mitochondrial Cytochrome c Oxidase. Cell reports, 29(12), 4114-4126.e5.
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9

Characterization of C18-4 Spermatogonial Stem Cell Line

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The C18-4 cell line was generously provided by Dr. Marie-Claude Hofmann (MD Anderson Cancer Center, Huston, TX, USA). This cell line was established from type A spermatogonia obtained from testes of 6-day-old mice.25 (link) The C18-4 cells express various markers for proliferating spermatogonia, and spermatogonial stem cells (SSCs), including germ cell nuclear antigen 1 (GCNA1), mouse ortholog of VASA, deleted in azoospermia-like (DAZL), proliferating cell nuclear antigen (PCNA), octamer-binding transcription factor 4 (OCT-4), glial cell line-derived neurotrophic factor family receptor alpha-1 (GFRA1), rearranged during transfection (RET), and promyelocytic leukemia zinc finger (PLZF).26 (link) This cell line was used in several studies as a model for spermatogonia stem cells.26 (link)27 (link)28 (link)29 (link) The C18-4 cell line was grown in DMEM media with 5% fetal bovine serum (FBS; 16140-071; Thermo Fisher, Waltham, MA, USA), 5% bovine growth serum (SH30541.03, Thermo Fisher), 1% penicillin/streptomycin (15140-122, Thermo Fisher), and 0.5% Fungizone (15290-018, Thermo Fisher) at 37°C with 5% CO2.
Vigodner M., Lucas B., Kemeny S., Schwartz T, & Levy R. (2020). Identification of sumoylated targets in proliferating mouse spermatogonia and human testicular seminomas. Asian Journal of Andrology, 22(6), 569-577.
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10

Immunofluorescence Staining of Cardiomyocytes

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Cells were fixed in 3.7% (v/v) paraformaldehyde for 10 min, then permeabilized and blocked in PBS with 4% (v/v) bovine growth serum (Thermo Scientific) and 0.2% (v/v) Triton X-100 for 1 h, and subsequently incubated with primary antibodies for AcH3 (Millipore), Actc1 (Sigma), sarcomeric MyHC (R&D), Nppa (Millipore), Ryr2 (Sigma), Pln (Abcam) in blocking buffer overnight on a rocker at 4 °C. Secondary antibody incubation (Alexa 647, Life Technologies) was performed in blocking buffer for 1 h at room temperature. The fixed PDMS membranes were then mounted on slides in Vectashield® mounting medium and analysed with confocal microcospy (Zeiss LSM-780 inverted) or conventional epifluoresence microscopy with a Zeiss Axio-Observer Z1 widefield fluorescent microscope.
High-throughput image analysis was performed with a custom made Matlab® script. Briefly, cell nuclei were detected using contrast enhancement methods, then segmented with an edge detection algorithm (Canny; Matlab®), following which elliptical fitting was performed to compute the aspect ratio (short axis/long axis) and the angle between the long axis and the groove direction.
Morez C., Noseda M., Paiva M.A., Belian E., Schneider M.D, & Stevens M.M. (2015). Enhanced efficiency of genetic programming toward cardiomyocyte creation through topographical cues. Biomaterials, 70, 94-104.
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