Anti nanog sc 33760

Manufactured by Santa Cruz Biotechnology

Anti-Nanog (sc-33760) is a primary antibody product offered by Santa Cruz Biotechnology. It is designed to detect the Nanog protein, which is a transcription factor involved in the self-renewal and pluripotency of embryonic stem cells. The antibody can be used in various immunodetection techniques, such as Western blotting, immunoprecipitation, and immunohistochemistry, to study the expression and localization of the Nanog protein in biological samples.

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Lab products found in correlation

Anti oct4 sc 8628, by Santa Cruz Biotechnology (2 mentions) Mab2736, by R&D Systems (2 mentions) Vectashield mounting medium with 4 6 diamidino 2 phenylindole dapi, by Vector Laboratories (1 mentions) Sc 25310, by Santa Cruz Biotechnology (1 mentions) Axio observer a1 inverted, by Zeiss (1 mentions) Anti rex1, by Santa Cruz Biotechnology (1 mentions) Anti sox2, by Santa Cruz Biotechnology (1 mentions) Anti gata4, by Santa Cruz Biotechnology (1 mentions) Ab5694, by Abcam (1 mentions) Fv1000, by Olympus (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Vector Laboratories (1 mentions) Anti nestin, by R&D Systems (1 mentions) Alexa fluor 594 lgg antibody, by Thermo Fisher Scientific (1 mentions) Vectashield mounting medium, by Vector Laboratories (1 mentions)

Spelling variants of this product

Nanog (81 citations) Anti-Nanog (37 citations)

2 protocols using anti nanog sc 33760

Immunofluorescence Assay for Stem Cell Markers

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Cells cultured in 24-well dishes were fixed in 4% paraformaldehyde and permeabilized with 0.25% Triton X-100, followed by blocking with 3% BSA in PBS. Then cells were probed with primary antibodyin 3% BSA for 1 h at 4°C and secondary antibodyin 3% BSA for 30 min at room temperature. A drop of Vectashield mounting medium with 4′, 6-diamidino-2-phenylindole (DAPI; Vector Laboratories) was placed on the microscope slide and the cover slip was sealed with nail polish in a way that the ES cells were in contact with the mounting medium. Staining signal was then observed through the Axio Observer A1 inverted light microscope (Zeiss). Primary antibody used were: anti-Oct4 (sc-8628, Santa Cruz), and anti-Nanog (sc-33760, Santa Cruz), anti-Sox2 (sc-99000, Santa Cruz), anti-Rex1 (sc-377095, Santa Cruz), anti-SSEA-1 (mab34301, Millipore), anti-alpha smooth muscle Actin (ab5694, Abcam), anti-Nestin (mab2736, R&D), anti-Gata4 (sc-25310, Santa Cruz).

Ma H., Ng H.M., Teh X., Li H., Lee Y.H., Chong Y.M., Loh Y.H., Collins J.J., Feng B., Yang H, & Wu Q. (2014). Zfp322a Regulates Mouse ES Cell Pluripotency and Enhances Reprogramming Efficiency. PLoS Genetics, 10(2), e1004038.

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Immunostaining of Pluripotent Stem Cells

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Cells were cultured on coverslips were fixed and then probed with primary antibody, washed with PBST, probed with secondary antibody in the dark and washed with PBST again. The cells were stained with Vectashield mounting medium containing 4′, 6- diamidino-2-phenylindole (DAPI; Vector Laboratories) before observed under a confocal microscope (Olympus FV1000). Primary antibodies used for probing iPS cells were anti-Oct4 (sc-8628, Santa Cruz), anti-SSEA-1 (mab34301, Millipore) and anti-Nanog (sc-33760, Santa Cruz). As for probing EBs, primary antibodies used were anti-alpha smooth muscle Actin (ab5694, Abcam), anti-Gata4 (sc-25310, Santa Cruz) and anti-Nestin (mab2736, R&D). Secondary antibodies used to probe iPS cells were Alexa Fluor 594 lgG antibody (Invitrogen) while Alexa Fluor 488 lgG antibody (Invitrogen) was used to probe EBs.

Ji G., Xiao X., Huang M, & Wu Q. (2022). Jmjd6 regulates ES cell homeostasis and enhances reprogramming efficiency. Heliyon, 8(3), e09105.

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