NADP/NADPH-Glo and GSH-Glo assay kits (Promega) were used to measure NADPH levels in 20,000 cells and glutathione levels in 50,000 cells per replicate following manufacturer's instructions. To measure total glutathione (GSH + GSSG), oxidized glutathione (GSSG) was reduced by incubating test samples with 500 μM tris(2-carboxyethyl)phosphine (TCEP) for 10 minutes prior to luminescence measurement, following the manufacturer's instructions. Each assay was read out using a Promega GloMax Explorer luminometer.
Nadp nadph glo and gsh glo assay kits
Manufactured by Promega
The NADP/NADPH-Glo and GSH-Glo assay kits are quantitative bioluminescent assay kits for measuring NADP/NADPH and glutathione (GSH) levels, respectively, in biological samples. The assays utilize a novel luciferase-based detection system to provide a sensitive and reliable measurement of these important cellular metabolites.
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Lab products found in correlation
2 protocols using nadp nadph glo and gsh glo assay kits
1
Quantifying Cellular NADPH and Glutathione
2
Quantifying Cellular NADPH and Glutathione
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