Pentyl acetate

Copper(II) nitrate hemipentahydrate (Cu(NO₃)₂ · 2.5 H₂O, ≥98.0%), ethylenediamine (EDA, ≥99.5%), hydrazine (35 wt% in H₂O), polyvinylpyrrolidone (PVP, avg MW 10 000), silver nitrate (AgNO₃, ≥99.0%), L‐ascorbic acid (99.0%), ethyl acetate, pentyl acetate, and nitrocellulose (NC) were all obtained from Sigma‐Aldrich. Sodium hydroxide (NaOH, 99.0%) was purchased from NOAH Technologies Corporation. N,N‐Diethylhydroxylamine (DEHA, >95.0%) was obtained from TCI America. Isopropyl alcohol (IPA, ACS reagent), acetone, ethanol, and toluene were purchased from BDH VWR International.

Flavor compounds in SFB samples were extracted as described previously (48 (link)) and quantified using a Clarus 500 gas chromatograph (PerkinElmer, Waltham, MA) equipped with flame ionization detectors (FIDs) and a 50-m by 0.25-mm by 0.2-μm CP-Wax 57 CB column (Agilent, Santa Clara, CA) as described previously (28 (link)). The compounds were quantitated by comparison with the internal standards 2-methyl-2-butanol, pentyl acetate, and 2-ethylbutyric acid (Sigma-Aldrich, Shanghai, China).

The following odors and agonists were used: pentyl acetate (Sigma-Aldrich, Cat. Nr. 109584, 99%), butyl acetate (Fluka, Cat. Nr. 45860, ≥99.5% (GC), ACS Reagent), methyl hexanoate (Fluka, Cat. Nr. 21599, 99.8%, analytical standard grade), propyl acetate (Sigma-Aldrich, Cat. Nr. 133108, 99%; Fluka, Cat. Nr. 40858, ≥99%, analytical standard grade), 3-methylthio-1-propanol (Sigma-Aldrich, Cat. Nr. 318396, 98%; Sigma-Aldrich, Cat. Nr. W341509, ≥98% synthetic, FG grade), hexyl acetate (Sigma-Aldrich, Cat. Nr. 108154, 99%), isobutyl acetate (Fluka, Cat. Nr. 94823, 99.8%, analytical standard grade), 2-heptanone (Sigma-Aldrich, Cat. Nr. W254401, 98% synthetic, FG grade), 3-octanol (Sigma-Aldrich, Cat. Nr. 218405, 99%), (±)-geosmin (Sigma-Adrich, Cat. Nr. UC18, ≥98% [GC]), VUAA1 (CAS Nr. 525582-84-7) was synthesized by the group “Mass Spectrometry/Proteomics” of the Max Planck Institute for Chemical Ecology (Jena, Germany). Stimuli stocks consisted of 100 mM solutions in dimethyl sulfoxide (DMSO, Sigma-Aldrich, Cat. Nr. D8418); working solutions were prepared fresh diluting 100 mM stocks in SES to the desired concentration just before the start of the experiment. Negative controls consisted of the equivalent maximum volume of DMSO used to prepare odor stimuli solutions diluted in SES.