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Apc anti human cd62l clone dreg 56

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APC-anti-human CD62L (clone DREG-56) is a fluorochrome-conjugated monoclonal antibody that binds to the CD62L (L-selectin) cell surface antigen expressed on various human leukocyte subsets. It is designed for use in flow cytometry applications.

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Lab products found in correlation

Pharm lyse solution, by BD (1 mentions) Lsr fortessa instrument, by BD (1 mentions)

Close spelling variants of this product

Anti-CD62L (clone DREG-56, (2 citations) CD62L (DREG-56, (4 citations) DREG-56 (5 citations) Anti-CD62L-APC (26 citations) CD62L-APC (28 citations) Anti-CD62L (31 citations) CD62L (98 citations)

2 protocols using apc anti human cd62l clone dreg 56

1

Neutrophil Activation Assay

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Heparin whole-blood samples (500 μL) were either kept on ice or incubated with PBS or 10−6 M bacterial peptide formyl-methionyl-leucyl-phenyl-alanine (fMLP) (Sigma Chemical Co., St Louis, MO) for 5 min. Samples were stained with PE-anti-human CD11b (clone 2LPM19c, Dakopatts, Glostrup, Denmark) and APC-anti-human CD62L (clone DREG-56, BD Biosciences) as previously reported [22 (link)]. Samples were then analyzed by means of flow cytometry, as described in the Additional file 1: Methods.
Loyer C., Lapostolle A., Urbina T., Elabbadi A., Lavillegrand J.R., Chaigneau T., Simoes C., Dessajan J., Desnos C., Morin-Brureau M., Chantran Y., Aucouturier P., Guidet B., Voiriot G., Ait-Oufella H, & Elbim C. (2022). Impairment of neutrophil functions and homeostasis in COVID-19 patients: association with disease severity. Critical Care, 26, 155.
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2

Activated Leukocyte Phenotyping by Flow

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PBMCs were stimulated for an hour at 37 °C with medium, LPS (100 ng/mL) used as a positive control or DC supernatants. Cells were stained at 4 °C for 15 min with an APC-anti-Human-CD62L (clone DREG-56, BD Pharmingen, 1:50), a BV650-anti-Human-CD11b (BioLegend, 1:120) and a PE-anti-Human-CD15 (BD Pharmingen, 1:20) or with the corresponding isotypes. Erythrocytes were lysed with 1X BD Pharm Lyse Solution (BD Pharmingen). White cells were resuspended in PBS supplemented with 1% human serum and 2 mM EDTA and analyzed on an LSR Fortessa instrument (BD Biosciences).
Noël F., Massenet-Regad L., Carmi-Levy I., Cappuccio A., Grandclaudon M., Trichot C., Kieffer Y., Mechta-Grigoriou F, & Soumelis V. (2021). Dissection of intercellular communication using the transcriptome-based framework ICELLNET. Nature Communications, 12, 1089.
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