Hrp conjugated anti mouse and anti rabbit antibodies

DMEM, RPMI, trypsin-EDTA 0.25% and penicillin-streptomycin (PS) was purchased from GE Healthcare Life Sciences Hyclone Laboratories (Pittsburg, PA, USA). Fetal bovine serum (FBS) was received from Gibco (Thermo Fisher Scientific Inc., Walthem, MA, USA). ApoScan^TM annexin V-FITC was obtained from BioBud (Cat. No.: LS-02-100, Seongnam, Gyeonggi-do, Korea). MTT cell viability assay kit EZ-CYTOX was acquired from Do-GenBio Co. Ltd. (Seoul, Korea). PP2A activity kit was purchased from Millipore Corporation (Billerica, MA, USA). Antibodies against AKT (9272), pAKT (Thr308) (13038), Caspase-3 (9662), BAX (2772), and PARP (9542), and Bcl-2 (3498) were provided from Cell Signaling Technology (Danvers, MA, USA). β-actin (sc-47778), cytochrome-c (sc-13156), ERK 1/2 (sc-514302), pERK (Thr202/Tyr204) (sc-136521), and HRP-conjugated anti-mouse and anti-rabbit antibodies were received from Santa Cruz Biotechnology (Dallas, TX, USA). SK1 (ab71700) and SK1 (ab264042) antibodies were acquired from Abcam (Cambridge, UK). Protein marker, protease, and phosphatase inhibitor cocktail were purchased from Thermo Fisher Scientific (Waltham, MA, USA). ECL Solution for western blotting imaging was received from Millipore Corporation (Burlington, MA, USA).

TACS® Annexin V Kit (Gaithersburg, MD, USA) was used for assaying cell apoptosis. Reagents for SEM were obtained from Electron Microscopy Sciences (Hatfield, PA, USA): glutaraldehyde, osmium tetroxide and uranyl acetate. Antibodies for human TRAIL were purchased from Biolegend (San Diego, CA, USA). Primary antibodies for human TRAIL and β-actin were obtained from PeproTech (Rocky Hill, NJ, USA) and Santa Cruz Biotech (Santa Cruz, CA, USA). HRP-conjugated anti-mouse and anti-rabbit antibodies were obtained from Santa Cruz Biotech. IL-8 human Elisa Kits were purchased from ThermoFisher.

Roswell Park Memorial Institute-1640 (RPMI-1640), trypsin, and penicillin/streptomycin (P/S) were supplied from GE Healthcare Life Sciences Hyclone Laboratories (Pittsburg, PA, USA). Fetal bovine serum (FBS) was purchased from Gibco (Thermo Fisher Scientific Inc., Waltham, MA, USA). ApoScan^TM annexin V-FITC was obtained from BioBud (Seongnam, Gyeonggi-do, Korea). Cell viability assay kit EZ-CYTOX was received from Do-GenBio Co. Ltd. (Seoul, Korea). Antibodies against Caspase-3 (9662), BAX (2772), PARP (9542), and Bcl-2 (3498) were provided by Cell Signaling Technology (Danvers, MA, USA). β-actin (sc-47778) and HRP-conjugated anti-mouse and anti-rabbit antibodies were supplied from Santa Cruz Biotechnology (Dallas, TX, USA). SK1 (ab71700) and SK2 (ab264042) antibodies were acquired from Abcam (Cambridge, UK). Protein marker, protease, and phosphatase inhibitor cocktail were received from Thermo Fisher Scientific (Waltham, MA, USA). Electrochemiluminescence Solution for Western blotting imaging was offered by Millipore Corporation (Burlington, MA, USA). Alanine transaminase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) kits were purchased from Asan Corporation (Seoul, Korea).