Ds ri1 ccd camera

Manufactured by Nikon

Sourced in United States

The DS-Ri1 CCD camera is a high-resolution digital camera designed for microscopy applications. It features a 12.7-megapixel CCD sensor and can capture images with a resolution of up to 4,080 x 3,072 pixels. The camera is capable of real-time image display and transfer, and it supports a variety of image file formats for convenient data management and analysis.

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Lab products found in correlation

Deadend fluorometric tunel system, by Promega (4 mentions) Confocal laser scanning microscope, by Zeiss (4 mentions) Ab49405, by Abcam (2 mentions) Eclipse 80i microscope, by Nikon (2 mentions) Nis elements microscope imaging software, by Nikon (2 mentions) 3β hsd, by Santa Cruz Biotechnology (2 mentions) Vectastain abc avidin biotin peroxidase kit, by Vector Laboratories (2 mentions) Smz1500 stereoscope, by Nikon (1 mentions) Ab13840, by Fortis Life Sciences (1 mentions) Ab15093, by Merck Group (1 mentions) Ab15580, by Abcam (1 mentions) Mca2336, by Abcam (1 mentions) 780 meta, by Zeiss (1 mentions) Vectastain abc kit, by Vector Laboratories (1 mentions) Ab15093, by Abcam (1 mentions) Ab15580, by Merck Group (1 mentions) Ab5535, by Abcam (1 mentions) Ab89901, by Abcam (1 mentions) Cy3 conjugated donkey anti rabbit igg, by Jackson ImmunoResearch (1 mentions) Fitc conjugated donkey anti goat igg, by Jackson ImmunoResearch (1 mentions)

18 protocols using ds ri1 ccd camera

Synthesizing Digoxigenin-Labeled RNA Probes for In Situ Hybridization

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Digoxigenin-UTP-labeled antisense RNA probes were synthesized in vitro using a linearized plasmid or PCR product as template. The templates for dlx3b and ntla were linearized as previously reported 31 (link), while those for alkbh4, atrn, mpi and ranbp10 were amplified with the following primers:
alkbh4:
5'-CTCCAGAAGAATGATCTGATTC-3' (forward) and
5'-TAATACGACTCACTATAGGGCACTTTACATTTGTGCAATTGAAC-3' (reverse);
atrn:
5'-GTGGCATTGGAGACGGACGAGGAGC-3' (forward) and
5'-TAATACGACTCACTATAGGGCAGTTAGCGCACCAAACATGCACAC-3' (reverse);
mpi:
5'-TCTGTCCGGAGACTGTGTGGAGTGTATGGC-3' (forward) and
5'-TAATACGACTCACTATAGGGGAAGCGTCTCCTACAGTAAGCAGCACGCTC-3' (reverse);
ranbp10:
5'-CTCATTTGCACAGCACAGGCGCAGACAGTC-3' (forward) and
5'-TAATACGACTCACTATAGGGGCTTCTGGCAAGCGGCACACCAATTC-3' (reverse).
Whole-mount in situ hybridization was performed as previously described 31 (link). Embryos after whole-mount in situ hybridization were immersed in glycerol and photographed using the Ds-Ri1 CCD camera under a Nikon SMZ1500 stereoscope.

Sun Q., Liu X., Gong B., Wu D., Meng A, & Jia S. (2017). Alkbh4 and Atrn Act Maternally to Regulate Zebrafish Epiboly. International Journal of Biological Sciences, 13(8), 1051-1066.

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Immunohistochemical and Immunofluorescence Analysis of Germ Cells

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IHC and IF procedures were performed as described previously [30 (link)]. Antibodies were diluted as follows: MVH (1:500, Abcam, ab13840), WDR62 (1:400, Bethyl, A301-560A), DAZL (1:100, AbD Serotec, MCA2336), STRA8 (1:200, Abcam, ab49405), SYCP3 (1:200, Abcam, ab15093), γH2AX (1:400, Millipore, 05–636), Ki67 (1:200, Abcam, ab15580). After staining, the sections were examined with a Nikon microscopy, and images were captured with a Nikon DS-Ri1 CCD camera. The IF sections were examined using a confocal laser scanning microscope (Carl Zeiss Inc., Thornwood, NY). TUNEL assay was performed using the DeadEnd Fluorometric TUNEL System (Promega, G3250).
For quantitative analyses, more than three biological replicates from the control and experimental groups were performed. Paraffin-embedded ovaries and testes were serially sectioned and at least three sections apart were stained for observation. Within the group, at least three cross sections from each animal were examined. Around 1000 germ cells were counted in each group. The quantification of germ cells number (GCs number) was normalized to the control group. We considered the number of germ cells in the control group as 1, then the number of germ cells in other groups were quantified relative to the number of germ cells in the control group.

Zhou Y., Qin Y., Qin Y., Xu B., Guo T., Ke H., Chen M., Zhang L., Han F., Li Y., Chen M., Behrens A., Wang Y., Xu Z., Chen Z.J, & Gao F. (2018). Wdr62 is involved in female meiotic initiation via activating JNK signaling and associated with POI in humans. PLoS Genetics, 14(8), e1007463.

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Measuring Chromosome Signals with Microscopy

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Images were captured using a Nikon DS Ri1 CCD camera attached to a Nikon ECLIPSE 80i microscope. Image adjustment and chromosomal straightening were carried out using ImageJ software. The length of the chromosome and the distance from the signal to the end of the chromosome were measured using NIS-Elements microscope imaging software (Nikon). The relative distance to the signal was calculated by the formula: Distance from the end of the short arm to the signal × 100 %/length of the pachytene chromosome.

Kuo Y.T., Hsu H.L., Yeh C.H, & Chang S.B. (2016). Application of a modified drop method for high-resolution pachytene chromosome spreads in two Phalaenopsis species. Molecular Cytogenetics, 9, 44.

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Apoptosis Detection in Testicular Cells

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Apoptosis detection of testicular cells was conducted with the Promega DeadEnd Fluorometric TUNEL System in accordance with the manufacturer's instructions. The paraffin‐embedded testis sections were assayed by the terminal deoxynucleotidyl transferase‐mediated deoxyuridine triphosphate nick end labeling (TUNEL) method to detect internucleosomal DNA fragmentation that is characteristic of apoptosis. The green fluorescence of apoptotic cells was detected in a blue background using the Nikon microscope, and the images were captured by the Nikon DS‐Ri1 CCD camera.

Liu Q., Cen C., Fu H., Wang F., Wang Y., Xu T, & Hou X. (2019). Antioxidant activity of Coridius chinensis extracts on manganese‐induced testicular damage in rats. Environmental Toxicology, 34(10), 1067-1073.

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Immunostaining of Ovarian Tissue

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Ovaries used for immunostaining were fixed in 4% paraformaldehyde (pH 7.4) overnight at 4 ℃, dehydrated, and embedded in paraffin. Paraffin-embedded ovaries were cut into sections of 5-μm thickness. Then, the sections were deparaffinized, immersed in sodium citrate buffer (pH 6.0), and heated for 15 min in a microwave for antigen retrieval. After blocking with 5% donkey serum albumin, sections were incubated with primary antibodies at 4 °C overnight. For immunohistochemistry, the sections were treated with 3% H₂O₂ to eliminate internal peroxidase activity and incubated with an appropriate horseradish peroxidase (HRP)-conjugated secondary antibody. Finally, the signal of primary antibody was detected by the Vectastain ABC kit (Vector Laboratories, CA, USA) and the sections were counterstained with hematoxylin. Images were captured using a Nikon DS-Ri1 CCD camera. For immunofluorescence, the sections were incubated with an appropriate FITC-conjugated secondary antibody. The nuclei were stained with DAPI. Images were captured using a laser scanning confocal microscope (Zeiss 780 META).

Liang Q.X., Wang Z.B., Lin F., Zhang C.H., Sun H.M., Zhou L., Zhou Q., Schatten H., Odile F.C., Brigitte B., Sun Q.Y, & Qian W.P. (2018). Ablation of beta subunit of protein kinase CK2 in mouse oocytes causes follicle atresia and premature ovarian failure. Cell Death & Disease, 9(5), 508.

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Apoptosis Detection in Testicular Tissue

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Apoptotic cells in testicular tissue were performed with DeadEnd™ Fluorometric TUNEL System (Promega). The paraffin‐embedded sections of testis were assayed by TUNEL method to detect internucleosomal DNA fragmentation that is characteristic of apoptosis. The apoptotic cells were labelled with green fluorescence signal in a blue background by a Nikon light microscope, and the images were captured by the Nikon DS‐Ri1 CCD camera.

Cen C., Wang F., Xiong K., Jiang L, & Hou X. (2021). Protective effects of Coridius chinensis extracts on rat reproductive damage induced by manganese. Andrologia, 54(2), e14326.

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TUNEL Assay for Cell Death

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TUNEL assays were conducted with the In Situ Cell Death Detection Kit, Fluorescein (Promega BioSciences, San Luis Obispo, CA, USA), as recommended. The images were captured by Nikon DS-Ri1 CCD camera.

Wang Y., Zhu T., Li Q., Liu C., Han F., Chen M., Zhang L., Cui X., Qin Y., Bao S, & Gao F. (2015). Prmt5 is required for germ cell survival during spermatogenesis in mice. Scientific Reports, 5, 11031.

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Immunohistochemistry and TUNEL Assay Protocol

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IHC and IF procedures were performed as described previously (Chen et al., 2019 (link); Qin et al., 2019 (link)). Antibodies were diluted as follows: MVH (1:500, Abcam, ab13840), PLZF (1:100, R&D, AF2944), PRMT5 (1:200, Millipore, 07-405), SOX9 (1:500, Millipore, AB5535), STRA8 (1:200, Abcam, ab49405), SYCP3 (1:200, Abcam, ab15093), Ki67 (1:400, Abcam, ab15580), and PH3 (1:400, Millipore, 2605439). After staining, the sections were examined with a Nikon microscopy, and images were captured with a Nikon DS-Ri1 CCD camera. The IF sections were examined using a confocal laser scanning microscope (Carl Zeiss Inc., Thornwood, NY, United States). TUNEL assay was performed using the Dead-End Fluorometric TUNEL System (Promega, G3250).

Dong F., Chen M., Chen M., Jiang L., Shen Z., Ma L., Han C., Guo X, & Gao F. (2021). PRMT5 Is Involved in Spermatogonial Stem Cells Maintenance by Regulating Plzf Expression via Modulation of Lysine Histone Modifications. Frontiers in Cell and Developmental Biology, 9, 673258.

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Immunohistochemistry and Follicle Counting Protocol

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Immunohistochemistry procedures were performed as described previously (Gao et al., 2006 (link)). Stained sections were examined with a Nikon microscope, and images were captured by a Nikon DS-Ri1 CCD camera. For immunofluorescence analysis, the 5 μm sections were incubated with 5% BSA in 0.3% Triton X-100 for 1 hr after rehydration and antigen retrieval. The sections were then incubated with the primary antibodies for 1.5 hr and the corresponding FITC-conjugated donkey anti-goat IgG (1:150, Jackson ImmunoResearch, 705-095-147) and Cy3-conjugated donkey anti-rabbit IgG (1:300, Jackson ImmunoResearch, 711-165-152) for 1 hr at room temperature. The following primary antibodies were used: WT1 (Abcam, ab89901), FOXL2 (Abcam, ab5096), CYP11A1 (Proteintech, 13363-1-AP), SF1 (Proteintech, 18658-1-AP), and 3β-HSD (Santa Cruz, sc-30820). After being washed three times in PBS, the nuclei were stained with DAPI. The sections were examined with a confocal laser scanning microscope (Carl Zeiss Inc, Thornwood, NY).
For follicle counting analysis, whole ovaries from control and Prmt5^flox/flox;Sf1^+/cre female mice at 2, 3, 4, and 5 weeks of age were serially sectioned at 5 μm thickness (n = 3/time point/genotype), and follicles were counted on every five sections.

Chen M., Dong F., Chen M., Shen Z., Wu H., Cen C., Cui X., Bao S, & Gao F. (2021). PRMT5 regulates ovarian follicle development by facilitating Wt1 translation. eLife, 10, e68930.

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Arabidopsis Leaf Structure Analysis

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For cellular structure analysis, Arabidopsis leaves were fixed in a solution containing 70% ethanol, 5% glacial acetic acid, and 3.7% formaldehyde for 24h at room temperature; this solution was then replaced twice with 70% ethanol. After dehydration through an ethanol series of 80, 90, and 100%, the fixed leaves were embedded into Technovit 7100 resin (Heraeus Kulzer) and polymerized at 37 °C for 3 d. The sample blocks were sectioned into 2 μm thick slices with a microtome (Leica) and stained with 0.25% toluidine blue O (Merck). Images were captured with a Nikon D40 camera or Nikon Eclipse80i microscope equipped with a Nikon DS-Ri1 CCD camera.

Li Q., Yin M., Li Y., Fan C., Yang Q., Wu J., Zhang C., Wang H, & Zhou Y. (2015). Expression of Brassica napus TTG2, a regulator of trichome development, increases plant sensitivity to salt stress by suppressing the expression of auxin biosynthesis genes. Journal of Experimental Botany, 66(19), 5821-5836.

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