Oxaliplatin (L-OHP, Jiangsu Hengrui, 50mg/pc), doxorubicin hydrochloride (ADM, Zhejiang Haizheng, 10mg/pc), 5-fluorouracil (5-FU, Jiangsu Nantong Jinghua Pharmacy, 0.25g/10ml), and VER (Verapamil, VER, Shanghai Hefeng Pharmacy, 5mg/2ml) were supplied by our hospital. The CCK-8 kit was purchased from Japan Dojindo Laboratories. The RNA extraction and reverse transcription kit were purchased from TIANGEN. 2×SYBR Green universal qPCR Master Mix was purchased from TIANGEN. Mouse-anti human CDKN2B/P15 and P-gP primary antibody were purchased from Abcam. The GADPH antibody was purchased from Sigma. The second antibody labeled by sheep-anti mouse HRP was purchased from Guizhou Jinqiao Biotechnology. The high-flux sequencing was performed by Guangzhou Ruibo. siRNA for gene transfection was purchased from Guizhou Ruibo. The overexpression plasmid and empty carrier TrueORF GOLD type were purchased from ORIGENE. The annexin V-PI double-staining kit was purchased from Beijing Beibo. The Lipofectamine 3000 was purchased from Invitrogen. Primer design and synthesis were performed by Shanghai Shanjing Biotechnology Company.
P gp primary antibody
Manufactured by Abcam
P-gP primary antibody is a lab equipment product used for the detection and analysis of P-glycoprotein (P-gP), a transmembrane protein involved in the efflux of various molecules from cells. This antibody can be utilized in various experimental techniques, such as Western blotting, immunohistochemistry, and flow cytometry, to study the expression and localization of P-gP in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Rna extraction and reverse transcription kit, by Tiangen Biotech (1 mentions)
Sybr green universal qpcr master mix, by Tiangen Biotech (1 mentions)
Gadph antibody, by Merck Group (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Phosphate buffer saline (pbs), by Thermo Fisher Scientific (1 mentions)
Prestoblue, by Thermo Fisher Scientific (1 mentions)
Leibovitz s l 15 medium, by Thermo Fisher Scientific (1 mentions)
Dcfda h2dcfda cellular ros assay kit, by Abcam (1 mentions)
Phalloidin ifluor 647 reagent, by Abcam (1 mentions)
Trypsin edta 0.25, by Thermo Fisher Scientific (1 mentions)
Polyvinyl alcohol (pva), by Merck Group (1 mentions)
Chitosan, by Merck Group (1 mentions)
Polyethylene glycol peg, by Merck Group (1 mentions)
2 protocols using p gp primary antibody
1
Oxaliplatin-Doxorubicin-5FU Combination Therapy
2
Multifunctional Polymeric Nanocarriers
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Acid terminated polylactic acid (PLA, MW 10,000–18,000), chitosan (MW 50 000–190 000 Da, 75%–85% degree of acetylation), polyethylene glycol (PEG) (MW 8500-11,500) and polyvinyl alcohol (PVA) were purchased from Sigma Aldrich (St. Louis, USA). Chloroform and dimethyl sulfoxide (DMSO) was purchased from Kanto Chemicals (Japan). Drugs including Bioperine was a kind gift from Sami Sabinsa Group, Sami Labs (Tokyo, Japan), verapamil hydrochloride (Ver) and doxorubicin hydrochloride (Dox) was purchased from TCI (Japan) and Cayman Chemicals (USA), respectively. Cell culture reagents including Leibovitz's L15 medium, Trypsin-EDTA (0.25%), penicillin (5000 U/ml)/streptomycin (5000 µg/ml), and phosphate buffer saline (PBS, pH 7.4) were procured from Gibco (Life Technologies). Cellular assay kit Presto Blue was procured from Invitrogen (USA). Phalloidin-iFluor 647 reagent, DCFDA/H2DCFDA cellular ROS assay kit, P-gp primary antibody and goat anti-rabbit Alexa-Fluor 594 secondary antibody was procured from Abcam.
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