Mice were housed in an 8- or 16-cage Promethion System (Sable Systems International, Las Vegas, NV). Mice were acclimated to the indirect calorimeter caging for 3 days in the weight-reduced state. After acclimatization, data was collected in the weight reduced state for 3 days and continued for 7 additional days while mice transitioned to the ad libitum feeding, weight regain phase. Intake, total energy expenditure (TEE), energy balance, resting energy expenditure (REE), non-resting energy expenditure (NREE), voluntary wheel running distance, and respiratory quotient (RQ) were collected. Weight reduced values were averaged across the 3 collection days. Data from the ad libitum refeeding period (7 days) is shown cumulatively across the regain period with the exception of RQ in which a daily average is provided.
Promethion system
Manufactured by Sable Systems
Sourced in United States
The Promethion system is a comprehensive suite of laboratory equipment designed for advanced environmental and metabolic research. It provides precise measurement and control of various environmental parameters, including temperature, humidity, and airflow. The Promethion system is a versatile tool that enables researchers to conduct detailed studies on the physiological responses of organisms under controlled conditions.
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Lab products found in correlation
29 protocols using promethion system
1
Indirect Calorimetry Monitoring of Mice
2
Sixteen-Channel Metabolic Phenotyping
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A sixteen-channel Promethion system (Sable Systems International) was used. Individual cages (interior dimensions of 31.5 × 15.5 (floor), up to 34.5 × 19.0 (ceiling) × 13.0 cm tall) include a ceiling-mounted food hopper (3 mg resolution) and water spigot. Cages include a ceiling-mounted small “hut” into which mice can climb, which permits body mass measurements. X- and Y-axis (horizontal plane) photoelectric beam motion detectors are positioned around the cage. A running wheel accessory was available but excluded from the current study. Air flow through the chamber was negative (2,000 mL/min) and gas analyses were recorded once per minute. Cages are mounted inside of thermally-controlled cabinets that are maintained at 30°C, to match parameters utilized in the OxyMax system.
3
Metabolic Monitoring of Mice
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A telemetric transmitter (weight, 1.1 g; volume, 0.52 mL; G2 E-mitter, Starr, Elst, The Netherlands) was implanted into the abdominal cavity after which the mice were allowed to recover for 2 weeks before they were individually housed in automated metabolic home cages (Promethion System; Sable Systems, Las Vegas, NV, USA) for another 5 days. The first 2 days were considered as acclimatization phase and were not included in the data analysis. Voluntary locomotor behavior (by infrared beam breaks), food intake, body temperature, O2 consumption, and CO2 production were continuously collected in 5 minute bins and energy expenditure and respiratory exchange ratio were calculated. Energy expenditure was not normalized to lean body mass.
4
Comprehensive Metabolic Phenotyping in Mice
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Indirect calorimetry and additional measures of food intake and physical activity were performed in open-circuit indirect calorimetry cages (Promethion System, Sable Systems International) at Vanderbilt Mouse Metabolic Phenotyping Center (MMPC). One week prior to the experiment start date, mice were singly housed for acclimation. On the day of the experiment start date, mice were weighed and body composition was assessed. The mice were placed in the cages of the Promethion System, one mouse per cage. The cages were housed in a light and temperature-controlled chamber. The light cycle was set on a 12:12 h cycle (6am–6 pm). Mice were left undisturbed for 5 days during which all the measurements were made. The system periodically measured rates of O2 consumption (VO2) and CO2 production (VCO2) for each cage, as well as food intake and physical activity (infrared beam array). Data were processed in time segments (daily 24 h, and 12 h dark/12 h light), and averages calculated for each as well as for the 24 h period. After 5 days, the mice were removed from the cages, and body composition and weight were measured again. Data was further processed using CalR (www.calrapp.org ).
5
Measuring Core Body Temperature in Mice
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To measure body temperature, transponders (G2 E-Mitter) were transplanted into the peritoneum of anaesthetized eight-week-old male mice. Then, 7 days after surgery, the recovered mice were single caged in a thermally and humidity-controlled environment using the Promethion system (Sable Systems). Body core temperatures were recorded at 6 °C as indicated in the figure legend.
6
Circadian Rhythm and Fitness Monitoring
7
Comprehensive Metabolic Phenotyping of Mice
8
Metabolic Monitoring in Animal Models
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Metabolic monitoring was conducted using the Comprehensive Laboratory Animal Monitoring System (CLAMS, Columbus Instruments at McMaster) or Promethion system (Sable Systems International, Novo Nordisk). The experiment was conducted after acclimatization to the system for 12 or 24 h. Food intake, physical activity (beam breaks), oxygen consumption (VO2), carbon dioxide production (VCO2), RER and energy expenditure data were collected every 20 min (CLAMS) or 5 min (Promethion) for the indicated periods. Fatty acid oxidation (mg per kg per h) was calculated using the following equation (1.70 × VO2 − 1.69 × VCO2). Carbohydrate oxidation (mg per kg per h) was calculated using the following equation (4.58 × VCO2 − 3.23 × VO2).
9
Comprehensive Metabolic Phenotyping in Mice
10
Measuring Food Intake and Muscle Weight in HF-fed Mice
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Food intake and gastrocnemius muscle weight were measured in HF-fed mice after 27 weeks of HF feeding. Average food intake for light and dark cycle was measured by a Promethion system (Sable Systems International). Wet gastrocnemius muscle was collected and weighed from mice fasted for 5 h.
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