Sta r max analyzer

Blood was collected at ICU admission for each patient on 0.109 mol/L trisodium citrate tube (BD Vacutainer, BD Diagnostics, Sparks, MD, USA). All analyses were performed on platelet-poor plasma obtained after a double centrifugation at 2500× g for 15 min at room temperature.
The following biomarkers were measured in the derivation and validation cohorts: Prothrombin time and fibrinogen were measured on an STA-R Max analyzer (Diagnostica Stago, Asnières-sur-Seine, France) using STA^® Neoplastin^® R (Diagnostica Stago) and STA^®Liquid-Fib (Diagnostica Stago); D-dimers levels were measured in µg/mL (fibrinogen equivalent units) using an immunoturbidimetric latex-particle assay (Liatest^® DDI-Plus, Diagnostica Stago) on the STA-R Max analyzer (Diagnostica Stago); and Von Willebrand factor antigen (VWF:Ag) was measured using an immunoturbidimetric assay (LIAPHEN^® VWF:Ag, HYPHEN BioMed, Andresy, France) on a CS 2400 analyzer (Sysmex, Kobe, Japan). Endocan was measured using the JDIYEK^® ELISA Kit (Biothelis, Lille, France). Other laboratory blood tests including a complete blood count, CRP, procalcitonin (PCT), liver transaminases, bilirubin, and creatinine were measured by standard methods as part of the patient’s care in the Biology and Pathology Center of Lille University Hospital.

We quantified the plasma FM level in platelet-poor plasma (STA-Liatest FM, Diagnostica Stago, France) using a STA-R Max analyzer (Diagnostica Stago, as recommended by the manufacturer). According to the manufacturer's recommendation and calibration curves, the limit of detection was 6 µg/mL. Furthermore, as part of a requirement of the COFRAC ISO 15189 label of our laboratory, the limit of quantification was determined in our coagulometers and was identified as 7 µg/mL. Hence, positivity for FM was indicated by values above 7 µg/mL. The local protocol for COVID-19 management suggested the physician to measure FM in COVID-19 patients at admission and thereafter 72 h.

Concentrations of dabigatran were estimated using one Ecarin chromogenic assay (STA-ECA-II, Diagnostica Stago), and concentrations of apixaban, edoxaban, and rivaroxaban with the corresponding procedure using the chromogenic assay (STA-liquid anti-Xa, Diagnostica Stago). All these procedures were performed on the STA-R MAX analyzer (Diagnostica Stago). The limit of quantification of the corresponding apixaban, dabigatran, edoxaban, and rivaroxaban assays were 15, 27, 20, and 25 ng/mL, respectively.
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