The following Abs were used: mouse anti-GPR15 (MAB3654) and anti-GRK5 (MAB4539) from R&D Systems; rabbit anti-GPR15 (NBP1-02651) from Novus Biologicals; mouse anti-Myc (05-724) from EMD Millipore; rabbit anti-Myc (sc-789), rabbit anti-GRK6 (sc-566), rabbit anti–β-tubulin (sc-9104), rabbit anti-GRK2 (sc-562), rabbit anti-GRK6 (sc-566), and rabbit anti–14-3-3β (sc-629) from Santa Cruz Biotechnology; rabbit anti–phospho-PKA substrate (p-PKA sub, 9624), rabbit anti–phospho-PKC substrate (p-PKC sub, 2261), rabbit anti-Rab7 (9367), rabbit anti-LAMP1 (9091), rabbit anti-EEA1 (3288), rabbit anti-PKA C-α (5842), and rabbit anti–β-arrestin1/2 (D24H9) from Cell Signaling Technology; sheep anti-TGN46 (AHP500GT) from AbD Serotec; AF488-mouse anti-HA (A-21287), rabbit anti-AF488 (A-11094), Cy3-goat anti-mouse immunoglobulin G (IgG; A10521), Cy3-goat anti-rabbit IgG (A10520), AF488–donkey anti-sheep IgG (A11015), AF647–goat anti-rabbit IgG (A21245), and RPE–goat anti-mouse IgG (M30004-1) from Life Technologies; horseradish peroxidase (HRP)–goat anti-rabbit IgG (PI-1000) and HRP–goat anti-mouse IgG (PI-2000) from Vector Laboratory; AF488-Fab fragment of goat anti-mouse IgG (115-547-003) and normal goat-anti mouse IgG (115-005-146) from Jackson ImmunoResearch Laboratories; AF488-streptavidin (405235) from BioLegend; normal goat serum from Thermo Fisher; and human AB serum from Sigma-Aldrich.
Sheep anti tgn46 ahp500gt
Manufactured by Bio-Rad
Sheep anti-TGN46 (AHP500GT) is a primary antibody produced in sheep, specific for the TGN46 (Transgolgi network protein 46) antigen. This antibody is designed for use in immunological techniques such as immunohistochemistry and Western blotting.
Automatically generated - may contain errors
Lab products found in correlation
Normal goat serum (ngs), by Thermo Fisher Scientific (1 mentions)
Rabbit anti β arrestin1 2 d24h9, by Cell Signaling Technology (1 mentions)
Human ab serum, by Merck Group (1 mentions)
Ab59680, by Abcam (1 mentions)
Ab2900, by Abcam (1 mentions)
Ab6789, by Abcam (1 mentions)
Ab6721, by Abcam (1 mentions)
Alexa fluor 488 555, by Thermo Fisher Scientific (1 mentions)
2 protocols using sheep anti tgn46 ahp500gt
1
Immunochemical Analysis of GPR15 and GRK
2
Immunolabeling Techniques for Protein Localization
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Monoclonal antibodies and their sources were as follows: Rabbit polyclonal anti-β-tubulin (ab59680; 1:500), rabbit anti-EEA1 (ab2900; 1:200), horseradish peroxidase (HRP)-conjugated goat polyclonal antibodies to rabbit IgG (ab6721; 1:10,000) and to mouse IgG (ab6789; 1:10,000) were from Abcam. Mouse monoclonal anti-CD63 (CLB180; 1:200) was from Zymed (Invitrogen). Sheep anti-TGN46 (AHP500GT, 1:400) was from AbD Serotec. Mouse monoclonal anti-TSG101 (GTX70255; 1:500) was from GeneTex. Sheep anti-EGFR (20-ES04; 1:500) was from Fitzgerald. Rabbit anti-TYRP1 (sc-25543; 1:200) was from Santa Cruz Biotechnology. Anti-Alix (1:1,000) antibody was a kind gift from Pr. Remy Sadoul. Secondary goat anti-rabbit or anti-mouse antibodies conjugated to Alexa Fluor-488, -555 or -647 were from Invitrogen (1:200). Protein A-conjugated to 10-nm gold particles was from Cell Microscopy Center (AZU, Utrecht University, Utrecht, the Netherlands).
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