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Tbxa2r

Manufactured by Cayman Chemical
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TBXA2R is a laboratory product manufactured by Cayman Chemical. It is a synthetic receptor that binds to thromboxane A2, a critical signaling molecule in various physiological processes. The product is designed for use in research applications to study the functions and mechanisms of the thromboxane A2 receptor.

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Lab products found in correlation

Tbxas1, by Cayman Chemical (5 mentions) Mpges 1, by Cayman Chemical (4 mentions) Abc complex kit, by Vector Laboratories (3 mentions) Cox 2, by Cell Signaling Technology (2 mentions)

6 protocols using tbxa2r

1

Histological and Immunohistochemical Analysis of Tissue Samples

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For histology, fixed tissues were embedded in paraffin, sectioned at 5 μm, and stained with hematoxylin and eosin according to standard protocols. Immunohistochemistry staining for TBXAS1 (#160715, Cayman Chemical Company; dilution 1:50), TBXA2R (#10004452, Cayman Chemical Company; dilution 1:50), mPGES1 (#160140, Cayman Chemical Company; dilution 1:50) and HER2 (HER2, #2242, Cell Signaling Technology; dilution 1:200) was performed using an ABC complex kit (PK-6100, Vector Laboratories, Burlingame, CA) following the manufacturer’s instructions. Sections were counterstained with Harris’s hematoxylin. Immunohistochemistry staining intensity was quantified by calculating the integrated optical density (IOD per mm2, sum) of the area of interest using the Image Pro-Plus 7.0 software program (Media Cybernetics, Bethesda, MD).
Li H., Lee M.H., Liu K., Wang T., Song M., Han Y., Yao K., Xie H., Zhu F., Grossmann M., Cleary M.P., Chen W., Bode A.M, & Dong Z. (2017). Inhibiting breast cancer by targeting the thromboxane A2 pathway. NPJ Precision Oncology, 1, 8.
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2

Antibody Sourcing for Prostaglandin Synthesis

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Primary antibodies against human microsomal prostaglandin E synthase-1 (mPGES1), thromboxane A2 synthase 1 (TBXAS1), and thromboxane A2 receptor (TBXA2R) were obtained from Cayman Chemical Company (Ann Arbor, MI). All chemicals were purchased from Sigma-Aldrich (St Louis, MO) unless otherwise specified.
Li H., Liu K., Boardman L.A., Zhao Y., Wang L., Sheng Y., Oi N., Limburg P.J., Bode A.M, & Dong Z. (2014). Circulating Prostaglandin Biosynthesis in Colorectal Cancer and Potential Clinical Significance. EBioMedicine, 2(2), 165-171.
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3

Evaluating Protein Expression in Breast Cancer

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Primary antibodies against human mPGES1, TBXAS1 and the TBXA2R were obtained from Cayman Chemical Company (Ann Arbor, MI). All other primary antibodies were purchased from Cell Signaling Technology (Beverly, MA). Human breast cancer tissue microarray slides (BC08023 and BR1503b) were purchased from US Biomax Inc. (Rockville, MD). Full-length cDNA for human Tbxas1 was obtained from Addgene Inc. (Cambridge, MA). The 29-mer small hairpin RNA constructs against human TBXAS1 and TBXA2R were purchased from Open Biosystems, Inc. (Huntsville, AL). All chemicals were purchased from Sigma-Aldrich (St Louis, MO) unless otherwise specified.
Li H., Lee M.H., Liu K., Wang T., Song M., Han Y., Yao K., Xie H., Zhu F., Grossmann M., Cleary M.P., Chen W., Bode A.M, & Dong Z. (2017). Inhibiting breast cancer by targeting the thromboxane A2 pathway. NPJ Precision Oncology, 1, 8.
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4

Immunohistochemical Analysis of Colon Cancer

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Surgically resected human colon tissues at all clinical stages were fixed in 10% formalin overnight at room temperature. For histology, fixed tissues were embedded in paraffin, sectioned at 5 μm, and stained with haematoxylin and eosin (H&E) according to standard protocols. Immunohistochemistry staining for human cyclooxygenase-2 (COX-2, #12282, Cell Signaling Technology; dilution 1:200), mPGES1 (#160140, Cayman Chemical Company; dilution 1:50), TBXAS1 (#160715, Cayman Chemical Company; dilution 1:50), TBXA2R (#10004452, Cayman Chemical Company; dilution 1:50), or Ki-67 (RM-9106, Thermo Scientific, Fremont, CA; dilution 1:200) was performed using an ABC complex kit (PK-6100, Vector Laboratories, Burlingame, CA) following the manufacturer’s instructions. Sections were counterstained with Harris’s haematoxylin. For antibody-negative controls, the primary antibodies were substituted with normal rabbit serum. Immunohistochemistry staining intensity was quantified by calculating the integrated optical density (IOD, sum) of the area of interest using the Image Pro-Plus 7.0 software program.
Li H., Liu K., Boardman L.A., Zhao Y., Wang L., Sheng Y., Oi N., Limburg P.J., Bode A.M, & Dong Z. (2014). Circulating Prostaglandin Biosynthesis in Colorectal Cancer and Potential Clinical Significance. EBioMedicine, 2(2), 165-171.
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5

Immunohistochemical Analysis of Colon Cancer

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Surgically resected human colon tissues at all clinical stages were fixed in 10% formalin overnight at room temperature. For histology, fixed tissues were embedded in paraffin, sectioned at 5 μm, and stained with hematoxylin and eosin (H&E) according to standard protocols. Immunohistochemistry staining for human cyclooxygenase-2 (COX-2, #12282, Cell Signaling Technology; dilution 1:200), mPGES1 (#160140, Cayman Chemical Company; dilution 1:50), TBXAS1 (#160715, Cayman Chemical Company; dilution 1:50), TBXA2R (#10004452, Cayman Chemical Company; dilution 1:50), or Ki-67 (RM-9106, Thermo Scientific, Fremont, CA; dilution 1:200) was performed using an ABC complex kit (PK-6100, Vector Laboratories, Burlingame, CA) following the manufacturer's instructions. Sections were counterstained with Harris's hematoxylin. For antibody-negative controls, the primary antibodies were substituted with normal rabbit serum. Immunohistochemistry staining intensity was quantified by calculating the integrated optical density (IOD, sum) of the area of interest using the Image Pro-Plus 7.0 software program.
Li H., Liu K., Boardman L.A., Zhao Y., Wang L., Sheng Y., Oi N., Limburg P.J., Bode A.M, & Dong Z. (2014). Circulating Prostaglandin Biosynthesis in Colorectal Cancer and Potential Clinical Significance. EBioMedicine, 2(2), 165-171.
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6

Prostaglandin and Thromboxane Antibody Sourcing

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Primary antibodies against human microsomal prostaglandin E synthase-1 (mPGES1), thromboxane A2 synthase 1 (TBXAS1), and thromboxane A2 receptor (TBXA2R) were obtained from Cayman Chemical Company (Ann Arbor, MI). All chemicals were purchased from Sigma-Aldrich (St Louis, MO) unless otherwise specified.
Li H., Liu K., Boardman L.A., Zhao Y., Wang L., Sheng Y., Oi N., Limburg P.J., Bode A.M, & Dong Z. (2014). Circulating Prostaglandin Biosynthesis in Colorectal Cancer and Potential Clinical Significance. EBioMedicine, 2(2), 165-171.
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