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EIF4E1 is a protein that plays a key role in the initiation of protein synthesis. It functions as a subunit of the eukaryotic translation initiation factor 4F (eIF4F) complex, which is responsible for recruiting the ribosome to the mRNA.

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Lab products found in correlation

Tnrc6a, by Novus Biologicals (1 mentions) Anti c myc agarose, by Merck Group (1 mentions) M7gtp sepharose, by GE Healthcare (1 mentions) Anti flag m2 affinity gel, by Merck Group (1 mentions) Anti hif 1α, by Cayman Chemical (1 mentions) P p70s6k, by Cell Signaling Technology (1 mentions) Hexokinase 2, by Cell Signaling Technology (1 mentions) P mtor, by Cell Signaling Technology (1 mentions) P70s6k, by Cell Signaling Technology (1 mentions) Anti gapdh, by Abcam (1 mentions)

2 protocols using eif4e1

1

Immunoprecipitation and Western Blot

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All the antibodies used in this report were commercially purchased: eIF4E1 (Santa Cruz, A-10, sc-271480), eIF4E2 (CST, D54C2), TNRC6A (Novus Biologicals, NBP1-28751), β-actin (GSGB-BIO, TA-09), IMP1 (Santa Cruz, sc-21026), PDCD4 (CST, D29C6), PTEN (Santa Cruz, A2B1, sc-7974), myc-specific mouse monoclonal antibody 9E10 (Santa Cruz Biotechnology, catalogue No. SC-40), Flag-specific mouse monoclonal antibody M2 (Sigma-Aldrich, catalogue No. F3165), Anti-Flag M2 affinity gel (Sigma), Anti-c-Myc Agarose (Sigma), M7-GTP-Sepharose (GE Healthcare Bioscience).
Chen S, & Gao G. (2017). MicroRNAs recruit eIF4E2 to repress translation of target mRNAs. Protein & Cell, 8(10), 750-761.
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2

Protein Expression Analysis by Western Blotting

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Western blotting was performed using standard methods. The following primary antibodies were used at a dilution of 1:1000: Anti-glucose transporter Glut-1 (abcam), Hexokinase II (Cell Signaling), Anti-Hif1α (Cayman), Anti-GAPDH (abcam), Anti-eIF4E1 (phospho S209) (abcam), eIF4E1 (Santa Cruz), Anti-Cytochrome C (EPR1327) (abcam), mTOR specific antibodies, rS6k, p-p70s6k, p70s6k and pmTOR were all from Cell Signaling.
Bhalla K., Jaber S., Nahid M. N., Underwood K., Beheshti A., Landon A., Bhandary B., Bastian P., Evens A.M., Haley J., Polster B, & Gartenhaus R.B. (2018). Role of hypoxia in Diffuse Large B-cell Lymphoma: Metabolic repression and selective translation of HK2 facilitates development of DLBCL. Scientific Reports, 8, 744.
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