Lg100268

Manufactured by Merck Group

Sourced in United States

The LG100268 is a laboratory instrument manufactured by Merck Group. It is designed for specific laboratory operations and processes. The core function of this product is to provide precise and reliable measurements or analysis, as required by various scientific and research applications. No further details about the intended use or capabilities of this product can be provided in an unbiased and factual manner.

Automatically generated - may contain errors

Lab products found in correlation

Bms753, by Merck Group (4 mentions) Bms195614, by Merck Group (4 mentions) T0901317, by Merck Group (3 mentions) Hx531, by Merck Group (3 mentions) Dexamethasone (dex), by Merck Group (3 mentions) T0070907, by Bio-Techne (2 mentions) Gw3965, by Merck Group (2 mentions) Gw9662, by Merck Group (2 mentions) Uvi3003, by Santa Cruz Biotechnology (2 mentions) Ttnpb, by Merck Group (2 mentions) Hx531, by Bio-Techne (2 mentions) Rosiglitazone, by Cayman Chemical (2 mentions) P nitrophenyl phosphate pnpp reagent, by Merck Group (2 mentions) T0070907, by Enzo Life Sciences (2 mentions) Nile red, by Merck Group (2 mentions) Hoechst 33342, by Merck Group (2 mentions) Flutamide, by Merck Group (1 mentions) Tbbpa, by Merck Group (1 mentions) Desmosterol, by Steraloids (1 mentions) Bafilomycin a1, by Merck Group (1 mentions)

18 protocols using lg100268

Screening Compounds for Nuclear Receptor Modulators

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Dexamethasone, isobutylmethylxanthine, Nile red, Hoechst 33342, HX531, LG100268 (LG), 2,4-di-tert-butylphenol (2,4-DTBP), 2,6-di-tert-butylphenol (2,6-DTBP), 2,4,6-tri-tert-butylphenol (2,4,6-TTBP), 1,3-di-tert-butylbenzene (1,3-DTBB), 1,3,5-tri-tert-butylbenzene (1,3,5-TTBB), LG100268, GW3065, T3, and TTNPB were purchased from Sigma-Aldrich. CD3254 was purchased from Tocris Bioscience. Rosiglitazone (ROSI) was purchased from Cayman Chemical Company. T0070907 was from Enzo Life Sciences. Dimethylsulfoxide (DMSO) was purchased from Thermo Fisher Scientific.

Ren X.M., Chang R.C., Huang Y., Amorim Amato A., Carivenc C., Grimaldi M., Kuo Y., Balaguer P., Bourguet W, & Blumberg B. (2023). 2,4-Di-tert-butylphenol Induces Adipogenesis in Human Mesenchymal Stem Cells by Activating Retinoid X Receptors. Endocrinology, 164(4), bqad021.

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Chemical Stock Solutions for Cell Assays

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Chemicals were purchased as follows: RSG (Sigma cat # R2408, ≥98%), tributyltin chloride (Aldrich cat # T50202, 96%), T0070907 (Tocris cat # 2301, >99%), GW9662 (Sigma cat # M6191, >98%), BPA (Sigma cat # 239658, >99%), TBBPA (Aldrich cat # 25,759–1, >99%), TCBPA (Aldrich cat # 330396, >99%), BPAF (TCI America cat # T0062, >99%), GW3965 (Sigma cat #G6295, ≥98%), E2 (Sigma cat # E8875, ≥98%), flutamide (Sigma cat # F9397, ≥99%), 1–850 (Millipore cat # 609315, ≥98%), DEX (Sigma cat # D1756, ≥98%), and LG100268 (Sigma cat # SML0279, ≥98%). Stock solutions were prepared in 100% DMSO (Sigma cat # D2650) and stored at −20 °C between uses.

Kassotis C.D., Masse L., Kim S., Schlezinger J.J., Webster T.F, & Stapleton H.M. (2017). Characterization of Adipogenic Chemicals in Three Different Cell Culture Systems: Implications for Reproducibility Based on Cell Source and Handling. Scientific Reports, 7, 42104.

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Molecular Reagents for Cell Signaling Studies

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GW3965, LG100268, Actinomycin D, T0901317, and Bafilomycin A1 were obtained from Sigma. Simvastatin salt and MG-132 were purchased from Calbiochem. 22(R)-hydroxycholesterol and desmosterol were acquired from Steraloids. Lipoprotein deficient serum (LPDS) was prepared as previously reported and confirmed to contain no lipoproteins [30 (link)]. All other reagents were purchased from Sigma.

Cook E.C., Nelson J.K., Sorrentino V., Koenis D., Moeton M., Scheij S., Ottenhoff R., Bleijlevens B., Loregger A, & Zelcer N. (2017). Identification of the ER-resident E3 ubiquitin ligase RNF145 as a novel LXR-regulated gene. PLoS ONE, 12(2), e0172721.

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Chemical Reagents for Biomedical Research

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Dimethyl sulfoxide (DMSO) was purchased from American Bioanalytical (Natick, MA, USA). 3,3′,4,4′,5-pentachlorobiphenyl (PCB126, purity: ≥ 97%) was purchased from Ultra Scientific (North Kingstown, RI, USA). Tributyltin chloride (TBT, purity: 96%) and LG100268 (purity: ≥ 98%) were purchased from Sigma Aldrich (St. Louis, MO, USA). S26948 (Purity: ≥ 99%) was purchased from Tocris Bioscience (Minneapolis, MN, USA). All other reagents were from Thermo Fisher Scientific (Suwanee, GA, USA) unless noted.

Crawford K.A., Clark B.W., Heiger-Bernays W.J., Karchner S.I., Hahn M.E., Nacci D.E, & Schlezinger J.J. (2019). Tributyltin disrupts fin development in Fundulus heteroclitus from both PCB-sensitive and resistant populations: Investigations of potential interactions between AHR and PPARγ. Aquatic toxicology (Amsterdam, Netherlands), 218, 105334.

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Molecular Mechanisms of Apoptosis Regulation

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PAC, ATRA, BMS753, LG100268, BMS195614, and verapamil were purchased from Sigma-Aldrich (St. Louis, MO, USA). UVI3003, 3,5-bis(trifluoro-methyl)pyrazole (BTP-2), and NSC-95397 and antibodies for phospho-MAPK kinase (p-MEK)-1/2, p-extracellular signal-regulated kinase- (pERK-) 1/2, ERK-1/2, p53, cyclin B1, caspase-3, and β-actin were supplied by Santa Cruz Biotechnology (Dallas, TX, USA). Antibodies for caspase-8 and caspase-9 were procured from Cell Signaling Technology (Danvers, MA, USA).

Nguyen Q.T., Hoang T.X., Ryu H., Oh K.H, & Kim J.Y. (2021). Synergistic Antiproliferative Effects of All-Trans Retinoic Acid and Paclitaxel on Autosomal Dominant Polycystic Kidney Disease Epithelial Cells. BioMed Research International, 2021, 1242916.

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Retinoid Receptor Agonists and Antagonists

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ATRA, RARα agonist BMS753, RARα antagonist BMS195614, retinoid X receptor (RXR) α agonist LG100268, and pan RXR antagonist UVI3003 were purchased from Sigma-Aldrich (St. Louis, MO, USA). Antibiotic-antimycotic, HEPES (4-(2-hydroxyethyl)-1-piperazine ethane sulfonic acid) buffer, and β-mercaptoethanol were purchased from Invitrogen Corp (Gibco BRL, MD, USA).

Vu H.T., Hoang T.X, & Kim J.Y. (2018). All-Trans Retinoic Acid Enhances Matrix Metalloproteinase 2 Expression and Secretion in Human Myeloid Leukemia THP-1 Cells. BioMed Research International, 2018, 5971080.

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Organoid Transcriptional Modulation by Retinoid Analogs

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24 hours after splitting, organoids were treated with either ENR – Vitamin A containing 0.1% final volume DMSO, ENR + Vitamin A containing 0.1% final volume DMSO 5μM all-trans-Retinoic acid (ATRA, Cayman Chemical), 10μM 9- cis-Retinoic acid (9-cisRA, Cayman Chemical), 1μM AGN193109 (Sigma Aldrich), 1μM NRX194204 (Axon Medchem), 1μM HX531 (Sigma Aldrich) or 10 μM LG100268 (Sigma Aldrich). Drugs were stored under reduced light conditions and exposure to light was minimized. Media containing compounds was refreshed after 24h. Total treatment time was 48h for all drugs. All drugs were dissolved in DMSO and added to the culture medium at working concentration in 0.1% final volume DMSO. After treatment, organoids were harvested using Organoid Harvesting Solution (Cultrex, R&D Systems) and pelleted. Pellets were snap frozen and stored at −80 C for RNA-sequencing, or cryopreserved to maintain native chromatin context for ATAC-sequencing.

Wester R.A., van Voorthuijsen L., Neikes H.K., Dijkstra J.J., Lamers L.A., Frölich S., van der Sande M., Logie C., Lindeboom R.G, & Vermeulen M. (2021). Retinoic acid signaling drives differentiation toward the absorptive lineage in colorectal cancer. iScience, 24(12), 103444.

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Western Blot Antibody Sourcing

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LG100268 was purchased from Sigma (St Louis, MO, USA). The antibodiy against p-p38 MAPK was purchased from Millipore (USA). Antibodies against p38 MAPK, PARP, ERK, p-ERK, and c-Myc were from Cell Signaling Technology (USA), Antibodies against Survivin and cyclinD1 were from Wanleibio (China). Goat anti-rabbit antibody, goat anti-mouse antibody and anti-β-actin antibody were purchased from Zhongshan Goldenbrige Biotechnology (China).

Xu T., Zhong L., Gan L.G., Xiao C.L., Shan Z.L., Yang R., Song H., Li L, & Liu B.Z. (2016). Effects of LG268 on Cell Proliferation and Apoptosis of NB4 Cells. International Journal of Medical Sciences, 13(7), 517-523.

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Lipid Signaling Pathway Regulation

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M-CSF and GM-CSF were purchased from Cell Signaling Technology (Danvers, MA). All FAs were from Nu-Chek Prep. PA (5 mM), SA (5 mM), OA (5 mM), LA (5 mM), eicosapentaenoic acid (EPA, 5mM) and α-linolenic acid (ALA) (2mM) were prepared with 2 mM of endotoxin-free BSA in PBS (Cat#: BP9705-100, FISHER Scientific), sonicated until dissolved, and filtered through 0.22mm sterile filter as we previously described (14 ,15 (link)). The specific reactive oxygen species (ROS) inhibitors 4-amino-2,4-pyrrolidine-dicarboxylic acid (APDC) and butylated hydroxyanisole (BHA), PPARδ agonist GW0742 and antagonist GSK0660, PPARγ agonist Rosiglitazone and antagonist GW9662, RXR agonist LG100268 and antagonist HX531 were purchased from Sigma-Aldrich. RAR agonist BM753 and antagonist BMS195640 were purchased from TOCRIS. Necrosis inhibitor IM54 and apoptosis inhibitor z-VAD-FMK were purchased from Enzo Life Sciences. TLR4 inhibitor Eritoran, STAT3 inhibitor NSC74859, NFҡB inhibitor CAPE, IKK inhibitor BMS-345541, Cer synthesis inhibitor FB1 (fumonisin B1) and SPT (serine palmitoyltransferase) were purchased from Cayman Chemical.

Zeng J., Zhang Y., Hao J., Sun Y., Liu S., Bernlohr D.A., Sauter E.R., Cleary M.P., Suttles J, & Li B. (2018). Stearic acid induces CD11c expression in proinflammatory macrophages via epidermal fatty acid binding protein. Journal of immunology (Baltimore, Md. : 1950), 200(10), 3407-3419.

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Generation and Validation of MEFs with Vhl Knockout

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Vhl^loxP/loxP MEFs were generated from 13.5 days post-coitum (d.p.c.) embryos of Vhl^loxP/loxP mice (JAX Labs). These MEF cells were immortalized by serial passaging in high glucose DMEM (Life Technologies) supplemented with 10% FBS (Sigma-Aldrich) and penicillin-streptomycin (Life Technologies). Immortalized MEF cells were transfected with a tamoxifen-inducible ER-Cre vector expressing Cre recombinase fused with a mutated ligand-binding domain for the human estrogen receptor (ER-Cre). Stable MEF cell lines expressing ER-Cre were generated by culturing in selection media containing 5 μg/ml blasticidin (Thermo Fisher Scientific, Waltham, MA). Vhl^flox/flox MEFs were treated with 3 μM 4-hydroxytamoxifen (Sigma-Aldrich) for 2 days for efficient Vhl knockout.
Compounds used for the primary screen (Table S1) were solubilized in DMSO and used at a final concentration of 10 µM. Bexarotene, was obtained from Sigma-Aldrich and Selleck Chem (Houston, TX); LG100268 and TTNPB were obtained from Sigma-Aldrich and used in the secondary validation studies in vitro at final concentrations of 0.1 µM, 0.3 µM and 1.0 µM doses (solubilized in DMSO) for 48 h as indicated for each individual treatment.

Chowdhury P., Powell R.T., Stephan C., Uray I.P., Talley T., Karki M., Tripathi D.N., Park Y.S., Mancini M.A., Davies P, & Dere R. (2018). Bexarotene – a novel modulator of AURKA and the primary cilium in VHL-deficient cells. Journal of Cell Science, 131(24), jcs219923.

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