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Cd27 lg 7f9

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The CD27 (LG.7F9) is a laboratory reagent produced by Thermo Fisher Scientific. It is a monoclonal antibody that binds to the CD27 cell surface receptor. The CD27 receptor is expressed on various cell types and plays a role in the immune system. The CD27 (LG.7F9) reagent can be used for research purposes in flow cytometry and other immunological applications.

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Lab products found in correlation

Ifn γ xmg1.2, by Thermo Fisher Scientific (2 mentions) Facscanto, by BD (2 mentions) Ly49g2 4d11, by BD (1 mentions) Facscalibur, by BD (1 mentions) Icos c398.4a, by BioLegend (1 mentions) Lysis buffer, by BioLegend (1 mentions) Cd86 24f, by BioLegend (1 mentions) Cd40 hm40 3, by BioLegend (1 mentions) Cd4 ox35, by Thermo Fisher Scientific (1 mentions) Aria 2 flow cytometer, by BD (1 mentions) Cd11b m1 70, by Thermo Fisher Scientific (1 mentions) Nk1.1 pk136, by Thermo Fisher Scientific (1 mentions) Cd3 17a2, by Thermo Fisher Scientific (1 mentions) Ly49h 3d10, by Thermo Fisher Scientific (1 mentions) Cytofix cytoperm solution, by BD (1 mentions) Ly49d 4e5, by Thermo Fisher Scientific (1 mentions) Vybrant fam caspase 3 7 assay kit, by Thermo Fisher Scientific (1 mentions) Cd107a 1d4b, by BD (1 mentions) Nkp46 29a1, by Thermo Fisher Scientific (1 mentions) Cd103 2e7, by BioLegend (1 mentions)

Close spelling variants of this product

Anti-Cd27 Monoclonal Antibody (LG.7F9) FITC

6 protocols using cd27 lg 7f9

1

Multi-parameter Flow Cytometry of Murine NK Cells

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Anti-mouse NK1.1 (PK136), CD3ε (145-2C11), IFN-γ (XMG1.2), NKG2D (A10), NKG2A/C/E (20d5), CD49b (DX5), CD122 (5H4), CD11b (M1/70), and CD27 (LG.7F9) were obtained from eBioscience. mAbs for Ly49A (A1), Ly49D (4E5), Ly49C/I (5E6), and Ly49G2 (4D11) were obtained from BD Biosciences. The antibodies were conjugated to fluorescein isothiocyanate, pacific blue, phycoerythrin, allophycocyanin, or peridinin chlorophyll protein. A standard flow cytometry analysis was performed using a FACS Calibur (BD Bioscience) with FlowJo software (TreeStar, Ashland, OR).
Yea S.S., So L., Mallya S., Lee J., Rajasekaran K., Malarkannan S, & Fruman D.A. (2014). Effects of Novel Isoform-Selective Phosphoinositide 3-Kinase Inhibitors on Natural Killer Cell Function. PLoS ONE, 9(6), e99486.
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2

Immunophenotyping of EAMG Rat Immune Cells

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On day 50 (peak of the disease) after induction of EAMG, rats were euthanized under deepening anesthesia. Spleens and inguinal lymph nodes were removed and the weights were measured. Then the spleens and inguinal lymph nodes were minced through a 70 μm cell strainer to prepare single-cell suspensions of mononuclear cells (MNCs) under aseptic conditions. Red blood cells in spleen MNCs were lysed with a lysis buffer (Biolegend) for 5 min. All specimens were coded to facilitate blind testing. The spleen or inguinal lymph nodes MNCs suspension with a final volume of 100 μl were labeled with corresponding monoclonal antibodies and incubated for 30 min at 4 °C in the dark. The following antibodies were used in the assay: CD3 (1F4; Biolegend), CD4 (OX35; eBioscience), B220 (HIS24; eBioscience), CD20 (SP32; Abcam), CD161 (10/78; BD Pharmingen), MHC II (HIS19; Biolegend), CD80 (3H5; Biolegend), CD86 (24F; Biolegend), CD40 (HM40-3; Biolegend), CD27 (LG.7F9; eBioscience), ICOS (C398.4A; Biolegend), CXCR5 (ERP8837; Abcam). Unconjugated primary antibodies were detected with Alexa Fluro 488-conjugated anti-rabbit IgG (Abcam). Samples were analyzed using an Aria II flow cytometer (BD).
Zhang P., Yang C.L., Du T., Liu Y.D., Ge M.R., Li H., Liu R.T., Wang C.C., Dou Y.C, & Duan R.S. (2021). Diabetes mellitus exacerbates experimental autoimmune myasthenia gravis via modulating both adaptive and innate immunity. Journal of Neuroinflammation, 18, 244.
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3

Multiparametric Flow Cytometry Analysis

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Flow cytometry data were acquired on a FACSCanto (BD Biosciences, San Diego, CA) and analyzed with FlowJo software (Tree Star, Ashland, OR). To determine expression of cell surface proteins, mAb were incubated at 4°C for 20–30 min and cells were fixed using Cytofix/Cytoperm Solution (BD Biosciences) and, in some instances followed by mAb incubation to detect intracellular proteins. The following mAb clones were used: NK1.1 (PK136, eBioscience), CD3 (17A2, eBioscience), Ly49H (3D10, eBioscience), Ly49D (4E5, eBioscience), NKp46 (29A1.4, eBioscience), CD27 (LG.7F9, eBioscience), CD11b (M1/70, eBioscience), IFN-γ (XMG1.2; eBioscience), Granzyme B (MHGB04, Invitrogen), CD107a (1D4B, BD Pharmingen), AKT1 (55/PKBa/AKT, BD Pharmigen), pS473 (M89-61, BD Pharmigen).
Intracellular cytokine staining: For direct ex vivo, staining cells were incubated for 1 additional hour in the presence of Brefeldin A (BFA) before surface and intracellular IFN-γ staining. For cytokine staining following in vitro stimulation BFA was added during the last hour of stimulation. Intracellular signaling staining: For detection of intracellular AKT and pAKT (pS473) cells were methanol fixed and permeabilized according to BD protocol. Apoptosis was evaluated using Vybrant FAM Caspase-3/7 Assay Kit (Invitrogen) according to manufacturer’s protocol.
Jensen I.J., Winborn C.S., Fosdick M.G., Shao P., Tremblay M.M., Shan Q., Tripathy S.K., Snyder C.M., Xue H.H., Griffith T.S., Houtman J.C, & Badovinac V.P. (2018). Polymicrobial sepsis influences NK-cell-mediated immunity by diminishing NK-cell-intrinsic receptor-mediated effector responses to viral ligands or infections. PLoS Pathogens, 14(10), e1007405.
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4

Comprehensive Flow Cytometry Staining

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Flow cytometry data were acquired on a FACSCanto or LSRII (BD Biosciences) and analyzed with FlowJo software (Tree Star). To determine the expression of cell surface proteins, monoclonal antibodies (mAb) were incubated at 4 °C for 20 to 30 min and cells were fixed using BD Cytofix (BD Biosciences). To stain intracellular proteins, after staining cell surface proteins, the cells were fixed using Cytofix/Cytoperm (BD Biosciences), followed by incubation with mAb for an additional 20 to 30 min. The following mAb clones were used to stain the samples: CD8a (53 to 6.7; eBioscience), CD11a (M17/4; BioLegend), Thy1.1 (HIS51; eBioscience), CD127 (eBioSB/199; eBioscience), CD62L (MEL-14; eBioscience), CD27 (LG.7F9; eBioscience), CD122 (TM-b1; eBioscience), CD69 (H1.2F3; BioLegend). CD103 (2E7; BioLegend), IFN-γ (XMG1.2; eBioscience), IL-2 (JES6-5H4; eBioscience), TNF-α (MP6-XT22; BioLegend), CCL4 (polyclonal goat; R&D Systems), and XCL-1 (polyclonal goat; R&D Systems)
Heidarian M., Jensen I.J., Kannan S.K., Pewe L.L., Hassert M., Park S., Xue H.H., Harty J.T, & Badovinac V.P. (2023). Sublethal whole-body irradiation induces permanent loss and dysfunction in pathogen-specific circulating memory CD8 T cell populations. Proceedings of the National Academy of Sciences of the United States of America, 120(27), e2302785120.
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5

Cytokine Profiling of MCMV-specific T cells

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Lymphocytes were stimulated in RPMI/FCS for 6 hours with the H-2d restricted MCMV CD8 peptide epitopes (SGPSRGRII) from the MCMV M18 (33 (link)) or (YPHFMPTNL) from the pp89 antigen. For antigen 85A cells were stimulated either with the pool of 58 15mer peptides or with 3 H2d restricted peptide peptides encoding the dominant CD4 85A99–118aa (TFLTSELPGWLQANRHVKPT) or CD8 85A70–78aa (MPVGGQSSF) and 85A145–152aa (YAGAMSGL) epitopes (30 , 34 (link), 35 (link)) (peptides were synthesized at the WIMM, Oxford, UK). Each peptide was used at 2μg/ml. After 1 hour at 37°C, Golgi Plug (BD Biosciences, Oxford, UK) was added according to the manufacturer’s instructions.
Cells were washed and incubated with anti-mouse CD16/CD32 (Clone 93) to block Fc binding (eBioscience, Hatfield, UK). Subsequently the cells were stained for CD4 (RM4-5), CD8 (53-6.7) (BD Bioscience), fixable live/dead dead cell stain, IFNγ (XMG1.2), IL-2 (JES6-5H4), TNFα (MP6-XT22), CD127 (A7R34), CD62L (MEL-14), KLRG1 (2F1) and CD27 (LG.7F9) (all eBioscience), using the BD Cytofix/Cytoperm kit according to the manufacturer’s instructions. Cells were run on a LSRII (BD Biosciences) and analyzed using FlowJo software (Tree Star Inc, Ashland, Oregon, USA). Background responses of unstimulated cells were subtracted from the stimulated responses.
Beverley P.C., Ruzsics Z., Hey A., Hutchings C., Boos S., Bolinger B., Marchi E., O'Hara G., Klenerman P., Koszinowski U.H, & Tchilian E.Z. (2014). A Novel Murine Cytomegalovirus Vaccine Vector Protects against Mycobacterium tuberculosis. The Journal of Immunology Author Choice, 193(5), 2306-2316.
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6

Murine Immune Cell Profiling

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Antibodies against CD16/CD32, CD45(I3/2.3), CD4(RM4-5), CD8a(53-6.7), CD49b(DX5), CD25(CD25-4E3), FOXP3(FJK-16s), CD19(1D3), CD11b(1CRF44), Ly6C(HK1.4), Ly6G(RB6-8C5), F4/80(BM8), CD3e(145-2C11), and CD27(LG.7F9) were purchased from eBioscience (California, USA). Flow cytometry was performed as described previously 18 (link).
Deng J., Yuan W., Tan Q., Wei X, & Ma J. (2022). Non-absorbable antibiotic treatment inhibits colorectal cancer liver metastasis by modulating deoxycholic acid metabolism by intestinal microbes. Journal of Cancer, 13(3), 764-774.
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