Anti rabbit igg pi 1000

Fucoidan (from Fucus vesiculosus), 3,4-dihydroxyl-L-phenylalanine (L-DOPA), cholera toxin (CT), 12-O-tetradecanoylphorbol-13-acetate (TPA), and mushroom tyrosinase were from Sigma-Aldrich Co. (St. Louis, MO, USA). Antibodies specific for phospho-ERK1/2 (Thr202/Tyr204, #9101S), total (phosphorylated and non-phosphorylated) ERK1/2 (#9102), phospho-specific Akt (Ser473, #9271), total Akt (#9916), phospho-CREB (ser133, #9198), and total-CREB (#9197) were purchased from Cell Signaling Technology (Beverly, MA, USA). Antibodies for tyrosinase (C-19) and actin (I-19) were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Microphthalmia Ab-1 (C5, MS-771-P0) was from NeoMarkers (Fremont, CA, USA). PD98059 was from Cell Signaling Technology. Secondary antibodies specific for anti-goat IgG (PI-9500), anti-mouse IgG (PI-2000), and anti-rabbit IgG (PI-1000) were purchased from Vector Laboratories (Burlingame, CA, USA).

Dulbecco's modified Eagle's medium (DMEM), antibiotic (penicillin, streptomycin), and trypsin-EDTA were purchased from WelGENE (Daegu, Korea). Fetal bovine serum (FBS) was purchased from Hyclone (Logan, UT, USA). Kojic acid, α-melanocyte-stimulating hormone (α-MSH), mushroom tyrosinase, and 3,4-dihydroxy-L-phenylalanine (L-DOPA) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Antibody specific for phospho-CREB (ser133, #9198) and total-CREB (#9197) was from Cell Signaling Technology (Beverly, MA, USA). Antibodies specific for tyrosinase (C-19) and actin (I-19) were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA), and microphthalmia (MITF) Ab-1 (C5, MS-771-P0) was obtained from NeoMarkers (Fremont, CA, USA). Secondary antibodies specific for anti-goat IgG (PI-9500), anti-mouse IgG (PI-2000), and anti-rabbit IgG (PI-1000) were purchased from Vector Laboratories (Burlingame, CA, USA).