Mouse anti flag clone m2 f1804

Polyclonal, affinity purified anti-SART3 was generated by SDIX (Newark, DE, USA) using DNA immunization of rabbits against a polypeptide (amino acids 1–163). Antibodies were used at the following dilutions: rabbit anti-C. elegans SART3 (Q5635) 1:2000, mouse anti-Actin (clone C4, MAB1501, Millipore, Billerica, MA, USA) 1:10 000, mouse anti-GFP (mixture of clones 7.1 and 13.1, Roche, Penzberg, Germany) 1:2000, mouse anti-FLAG (clone M2, F1804, Sigma–Aldrich, St Louis, MO, USA) 1:2000. Western blotting was performed as previously described (38 (link)). Band intensities were quantified using ImageJ software (NIH, Bethesda, MD, USA).

Rabbit anti-LC3 (L8918), mouse anti-alpha-tubulin (T6199) and mouse anti-FLAG^® clone M2 (F1804) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Rabbit anti-SQSTM1/p62 (PA5-20839) was from ThermoFisher Scientific (Waltham, MA, USA). Rabbit anti-procaspase-3 (#9665), rabbit anti-cleaved-caspase-3 (Asp-175, clone 5A1E) (#9664), mouse anti-procaspase-9 (#9508), rabbit anti-PARP (#9542), rabbit anti-cleaved-PARP (#5625), rabbit anti-Atg5 (#12964) were from Cell Signaling (Danvers, MA, USA). Mouse anti-cytochrome c (7H8) (sc-13560) was from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Mouse anti-GFP (Ab1218) was from Abcam (Cambridge, UK). Rabbit and mouse horseradish-peroxidase-conjugated secondary antibody were from Cell Signaling. FITC-conjugated secondary antibody Alexa Fluor 488, MitoTracker Orange CMTM Ros (mitochondrial selective dye) (M7510) and the LysoTracker Green DND-26 (lysosomal selective dye) (L7526) were acquired from Molecular Probes (ThermoFisher Scientific). Trehalose (T0167), rapamycin (R0395), chloroquine (CQ) (C6628), ammonium chloride (NH₄Cl) and docetaxel were purchased from Sigma-Aldrich. 3-MA (S2767) was obtained from Selleckchem (Munich, Germany).