uHPLC–MS analysis was achieved using a Waters Acquity uHPLC provided of a ZQ 2000 ESI mass spectrometry (Waters, Milford, MA, USA) and Mass Link software (Waters, Milford, MA, USA). The 2.6 µm Kinetex 50 mm × 4.6 mm C18 column was selected to perform the analysis. The mobile phases used were water with 0.1% formic acid as solvent A and acetonitrile with 0.1% formic acid as solvent B. The liquid chromatography ran in a gradient condition from 100% H2O at t0 to 100% acetonitrile at t5 (5 min) under a flow rate of 0.3 mL/min. The column operated at a stationary temperature of 40 °C. Temperature, nebulizer pressure, and flow rate of drying gas (N2) were 230 °C, 35 psi, and 10 L/min, respectively. The further operation parameters were 1200V for nozzle voltage and 2500 V for the capillary voltage. Mass spectra were tracked in a mass-to-charge (m/z) ratio range of 150–500 in positive ion detection mode.
Acquity uhplc
Manufactured by Waters Corporation
Sourced in United States
The Acquity UHPLC is a high-performance liquid chromatography (HPLC) system developed by Waters Corporation. It utilizes ultra-high pressure technology to enable rapid, efficient, and sensitive separation and analysis of a wide range of chemical compounds.
Automatically generated - may contain errors
Lab products found in correlation
G2 q tof mass spectrometer, by Waters Corporation (3 mentions)
Avance neo 600, by Bruker (3 mentions)
Zq 2000 esi mass spectrometry, by Waters Corporation (2 mentions)
Mass link software, by Waters Corporation (2 mentions)
Triple tof 5600 system, by AB Sciex (2 mentions)
Avance 500, by Bruker (2 mentions)
Strata x, by Phenomenex (1 mentions)
Xevo tq s ms ms system, by Waters Corporation (1 mentions)
Tqs micro tandem quadrupole mass spectrometer, by Waters Corporation (1 mentions)
Beh c18 column, by Waters Corporation (1 mentions)
Xevo tqd, by Waters Corporation (1 mentions)
Q exactive hybrid quadrupole orbitrap mass spectrometer, by Thermo Fisher Scientific (1 mentions)
Acquity uplc, by Waters Corporation (1 mentions)
Waters xevo tq xs mass spectrometer, by Waters Corporation (1 mentions)
Impact 2 q tof mass spectrometer, by Bruker (1 mentions)
Agilent 1200 series hplc, by Agilent Technologies (1 mentions)
Xevo tq ms, by Waters Corporation (1 mentions)
Bond elut, by Agilent Technologies (1 mentions)
Bradford assay, by Thermo Fisher Scientific (1 mentions)
Optima lc ms grade, by Thermo Fisher Scientific (1 mentions)
19 protocols using acquity uhplc
1
Quantitative Profiling of Metabolites
2
UHPLC-MS/MS Metabolite Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
An Acquity UHPLC (Waters Corporation, Milford, MA, United States) was coupled to an AB SCIEX 5600 TripleTOF System (AB SCIEX, Framingham, MA, United States) (Q Exactive Orbitrap, Thermo Fisher Technologies, Waltham, MA, United States). The LC-MS conditions were described previously (Xiong et al., 2019 (link)).
3
Quantification of Kynurenine Pathway Metabolites
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Concentrations of TRP, l -kynurenine (KYN), 3-hydroxykynurenine (HK), anthranilic acid (AA), kynurenic acid, xanthurenic acid (XA), quinolinic acid, picolinic acid, and nicotinic acid, were determined at the department of Laboratory Medicine of the University Medical Center Groningen by liquid chromatography in combination with isotope dilution tandem mass spectrometry (LC–MS/MS) essentially as described by [33 (link)]. In short, 100 µL of CSF or 50 µL of serum was mixed with deuterated or 13C-labeled internal standards of the KP compounds under investigation. These mixtures were extracted with Strata X-A 96-well plates with a pore size of 33 µm and sorbent mass of 30 mg/well (Cat. No.: 8E-S123-TGB; Strata-X, Phenomenex, Utrecht, the Netherlands) and eluted with 3 M HCl in 1-butanol. Finally, 1 µL was injected into an Acquity UHPLC (Waters, Etten-Leur, the Netherlands) equipped with a Phenomenex Luna column (Omega C18, 100 × 2.1 mm, particle size 1.6 µm), and coupled to a XEVO TQ-S MS/MS system (Waters, Etten-Leur, the Netherlands). KYN/TRP ratios were calculated as a measure for TDO and IDO activity [34 (link)] and HK/XA ratios as a measure for vitamin B6 function since the enzyme converting HK to XA, kynurenine aminotransferase II, is pyridoxal phosphate-dependent [35 (link)].
4
Quantification of Atropine in Plasma
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Atropine concentration in plasma was quantified at the National Veterinary Institute (SVA) in Uppsala, Sweden, using ultra–high-performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS). The system was composed of an Acquity UHPLC coupled to a TQS Micro tandem quadrupole mass spectrometer with an electrospray interface operating in the positive mode (Waters Corporation, Milford, MA, United States). The calibration range was 0.05–60 μg/L plasma. The precision (relative standard deviation) was in the range of 2.1–8.3% and the recovery was 95.5–98%. The analytical method is thoroughly described in Ström et al.'s work (5 (link)).
5
Efficient Solvent-Free Synthetic Protocols
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All anhydrous solvents
and reagents were obtained from commercial suppliers and used without
further purification. Evaporation of solvent was carried out using
a rotary evaporator under reduced pressure at a bath temperature of
up to 60 °C. Flash column chromatography was carried out using
a Biotage purification system using SNAP KP-Sil cartridges or on reverse-phase
mode using SNAP Ultra C18 cartridges. Semipreparative separations
were carried out using an Agilent 1200 series preparative HPLC instrument
over a 15 min gradient elution. Microwave-assisted reactions were
carried out using a Biotage Initiator microwave system. Final compounds
were purified to ≥95% purity. NMR data were collected on a
Bruker Avance 500 spectrometer equipped with a 5 mm BBO/QNP probe
or on a Bruker Avance Neo 600 spectrometer equipped with a 5 mm TCI
CryoProbe. NMR data are presented in the form of chemical shift δ
(multiplicity, coupling constants, integration) for major diagnostic
protons, given in parts per million (ppm) relative to tetramethylsilane
(TMS), referenced to the internal deuterated solvent. HRMS was assessed
using an Agilent 1200 series HPLC instrument and diode array detector
coupled to a 6120 time-of-flight mass spectrometer with dual multimode
APCI/ESI source or on a Waters Acquity UHPLC and diode array detector
coupled to a Waters G2 QToF mass spectrometer fitted with a multimode
ESI/APCI source.
and reagents were obtained from commercial suppliers and used without
further purification. Evaporation of solvent was carried out using
a rotary evaporator under reduced pressure at a bath temperature of
up to 60 °C. Flash column chromatography was carried out using
a Biotage purification system using SNAP KP-Sil cartridges or on reverse-phase
mode using SNAP Ultra C18 cartridges. Semipreparative separations
were carried out using an Agilent 1200 series preparative HPLC instrument
over a 15 min gradient elution. Microwave-assisted reactions were
carried out using a Biotage Initiator microwave system. Final compounds
were purified to ≥95% purity. NMR data were collected on a
Bruker Avance 500 spectrometer equipped with a 5 mm BBO/QNP probe
or on a Bruker Avance Neo 600 spectrometer equipped with a 5 mm TCI
CryoProbe. NMR data are presented in the form of chemical shift δ
(multiplicity, coupling constants, integration) for major diagnostic
protons, given in parts per million (ppm) relative to tetramethylsilane
(TMS), referenced to the internal deuterated solvent. HRMS was assessed
using an Agilent 1200 series HPLC instrument and diode array detector
coupled to a 6120 time-of-flight mass spectrometer with dual multimode
APCI/ESI source or on a Waters Acquity UHPLC and diode array detector
coupled to a Waters G2 QToF mass spectrometer fitted with a multimode
ESI/APCI source.
6
UPLC-PDA-Based Multianalyte Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The quantitative analysis was carried out by the Waters Acquity UHPLC instrument (Waters Corp., Milford, MA, USA) using with PDA. The chromatography column, mobile phase and flow rate setting were as same as UPLC-Q-TOF/MS method. The column temperature was 40°C. The gradient elution program was set as: 0–2.5 min, 5%–10% B; 2.5–5.5 min, 10%–11% B; 5.5–6 min, 11%–12% B; 6–10 min, 12%–15% B; 10–13 min, 15%–17% B; 13–14 min, 17%–25% B; 14–15 min, 25%–5% B. The post run was 3 min. The optimal absorbed wavelengths were respectively 240 nm for geniposidic acid, geniposide, genipin, and pinoresinol di-o-glucopyranoside; 227 nm for syringaresinol di-o-glucopyranoside; and 327 nm for neochlorogenic acid, chlorogenic acid, caffeic acid, isochlorogenic acid A, and isochlorogenic acid C. In order to make the quantitative analysis more convenient, the multiwavelengths switch method was employed and was set as follows: 1.20–2.15 min, 240–327 nm; 2.15–4.50 min, 327–240 nm; 4.50–7.15 min, 240–227 nm; 7.15–10 min, 227–327; 10–12.24 min, 327–227 nm; 12.24–13.00 min, 227–327 nm. The injection volume was 3 µl.
7
Purification and Characterization of Organic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All anhydrous solvents
and reagents were obtained from commercial suppliers and used without
further purification. Evaporation of the solvent was carried out using
a rotary evaporator at reduced pressure at a bath temperature of up
to 60 °C. Flash column chromatography was carried out using a
Biotage purification system using SNAP KP-Sil or Sfar cartridges or
in the reverse-phase mode using SNAP Ultra C18 cartridges. Semipreparative
separations were carried out using a 1200 Series preparative HPLC
over a 15 min gradient elution. Microwave-assisted reactions were
carried out using a Biotage Initiator microwave system. The final
compounds were purified to ≥95% purity. NMR data were collected
on a Bruker Avance 500 spectrometer equipped with a 5 mm BBO/QNP probe
or on a Bruker Avance Neo 600 spectrometer equipped with a 5 mm TCI
Cryo-Probe. NMR data are presented in the form of chemical shift δ
(multiplicity, coupling constants, and integration) for major diagnostic
protons, given in parts per million (ppm) relative to tetramethylsilane
(TMS), referenced to the internal deuterated solvent. HRMS was assessed
using an Agilent 1200 series HPLC and diode array detector coupled
to a 6120 time of flight mass spectrometer with a dual multimode APCI/ESI
source or on a Waters Acquity UHPLC and diode array detector coupled
to a Waters G2 QToF mass spectrometer fitted with a multimode ESI/APCI
source.
and reagents were obtained from commercial suppliers and used without
further purification. Evaporation of the solvent was carried out using
a rotary evaporator at reduced pressure at a bath temperature of up
to 60 °C. Flash column chromatography was carried out using a
Biotage purification system using SNAP KP-Sil or Sfar cartridges or
in the reverse-phase mode using SNAP Ultra C18 cartridges. Semipreparative
separations were carried out using a 1200 Series preparative HPLC
over a 15 min gradient elution. Microwave-assisted reactions were
carried out using a Biotage Initiator microwave system. The final
compounds were purified to ≥95% purity. NMR data were collected
on a Bruker Avance 500 spectrometer equipped with a 5 mm BBO/QNP probe
or on a Bruker Avance Neo 600 spectrometer equipped with a 5 mm TCI
Cryo-Probe. NMR data are presented in the form of chemical shift δ
(multiplicity, coupling constants, and integration) for major diagnostic
protons, given in parts per million (ppm) relative to tetramethylsilane
(TMS), referenced to the internal deuterated solvent. HRMS was assessed
using an Agilent 1200 series HPLC and diode array detector coupled
to a 6120 time of flight mass spectrometer with a dual multimode APCI/ESI
source or on a Waters Acquity UHPLC and diode array detector coupled
to a Waters G2 QToF mass spectrometer fitted with a multimode ESI/APCI
source.
8
UHPLC-MS/MS Lipid Profiling Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The system used is a Waters Acquity UHPLC with a 2.1mm x 50mm Acquity BEH-C18 column held at 45°C. Method is 1% B held for 3 minutes then ramped to 60% B over 10 minutes (where B is methanol and A is 100 mM 1,1,1,3,3,3-hexafluoro- 2-propanol [HFIP] in 8.6 mM triethylamine, pH 9). Molecular weight identity was determined with a Micromass Quattro Micro MS/MS using an electrospray ion source in negative mode.
9
High-Resolution LC-MS Metabolomics
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
An Acquity UHPLC (Waters Corporation, Milford, MA, USA) was coupled with an AB SCIEX 5600 TripleTOF System (AB SCIEX, Framingham, MA, USA) (Q Exactive Orbitrap, Thermo Fisher Technologies, Waltham, MA, USA). The conditions for the LC-MS were followed as described previously [85 (link)].
10
Quantifying Cortisol and Cortisone via UHPLC-MS/MS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
An atmospheric pressure ionization (API) interface, an Acquity UHPLC with integrated solvent and sample management, and a tandem mass spectrometer (MS/MS) Xevo-TQD were all used in the investigation (Waters Corporation, Milford, MA, USA). The analytes were estimated using the Atlantis dC18 (2.1 × 100 mm, 3 μm) steel column, which was shielded by an in-line filter. The mobile phase, which was a 50 : 50 (v/v) mixture of acetonitrile and 2.0 mM ammonium acetate, was filtered through a supor membrane filter before being delivered (0.3 mL/minute) (Pall Gelman Laboratory, MI, USA). The electrospray ionization source was used to operate the mass spectrometer instrument in a positive ion mode (capillary voltage, 1.50 kV; cone voltage, 35 volts). While nitrogen (1000 L/hr) was utilized for nebulization and desolvation, argon was employed for collision (3.6 × 10−3 mbar). The optimum collision energy for cortisol, cortisone, and the IS was found to be 20 eV. The ion source and desolvation temperatures were kept at 150°C and 500°C, respectively. Multiple-reaction monitoring (MRM) was used to detect cortisol, cortisone, and IS in positive ion mode at mass-to-charge transitions (m/z) of 363.11 to 121.00, 361.18 to 163.111, and 367.19 to 121.24 for cortisol, cortisone, and IS, respectively.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!