Tcs sp8 x smd confocal microscope

We acquired images with Leica TCS SP8 X SMD confocal microscope (Leica Microsystem, Germany). Image acquisition was performed using a white light laser (WLL) with the following parameters: 1024 × 1024-pixel resolution, 400Hz, bidirectional mode, and zoom 8–12. For 3D projections, we obtained 30–40 optical sections with the axial step of 0.3 μm. Reconstruction of 3D projection was conducted using the Leica Application Suite (LAS) software.
Stimulated emission depletion (STED) microscopy was performed on the TCS SP8 STED 3X (Leica Microsystems) equipped with 660 nm and 775 nm STED lasers, STED White Objective CS 100 ×/1.40 OIL. Image acquisition was performed using depletion laser of 775 nm and 3X–3D STED, gating 0.3–6 ns for both Abberior STAR 580 and Abberior STAR 635P detection. Images were acquired using LAS X software and deconvolved by Huygens Professional software.

We acquired images with a Leica TCS SP8X SMD confocal microscope (Leica Microsystem, Wetzlar, Germany), equipped with 63× oil objective (HCX PL APO, lambda blue) with a numerical aperture (NA) 1.4. Image acquisition was performed using a white light laser (WLL; wavelengths of 470–670 nm in 1-nm increments) with the following parameters: 1024 × 1024-pixel resolution, 400 Hz, bidirectional mode, and zoom 8. For 3D projections, we acquired 30–40 optical sections with an axial step of 0.2 μm. Reconstruction of 3D projection was done using the Leica Application Suite (LAS X) software, and the Leica Lightning software performed the deconvolution procedure.

We used a Leica TCS SP8-X SMD confocal microscope (Leica Microsystems, Germany), equipped with a 63× oil objective (HCX PL APO; lambda blue) with a numerical aperture (NA) = 1.4. Image acquisition was performed using a white light laser (WLL; wavelengths of 470-670 nm in 1-nm increments) with the following parameters: 1024 × 1024-pixel resolution, 400 Hz, bidirectional mode, and zoom 8-12 using the Leica Application Suite (LAS X) software.