Si hnrnpa2b1

Protease inhibitor (PMSF, Beyotime, China); Cell RIPA lysate (Beyotime, China); Cell nuclear staining solution dihydrochloride (DAPI, Beyotime, China). Antibodies: anti-VP1 (gift from Zhenjiang First People's Hospital), anti-HNRNPA2B1 (Abcam, USA), anti-GAPDH (Abclonal, China), anti-histone (Abcam, USA), anti-tubulin (Abcam, USA); anti-HRP-IgG (Abcam, USA), anti-IgG H&L (Alexa Fluor® 594) fluorescent secondary antibody (Abcam, USA), Nuclear Plasma Isolation Kit (Beyotime, China), Lipofectamine^TM 2000 Transfection Reagent (Thermo Fisher Scientific, USA), si-HNRNPA2B1 (Ribobio, China).
Primer: EV-71: forward, 5’-GCTCTATAGGAGATAGTGTGAGTAGGG-3’, and reverse, 5’-ATGACTGCTCACCTGCGTGTT-3’. HNRNPA2B1: forward, 5’-GCTTAAGCTTTGAAACCACAGA-3’; and reverse, 5’-CTTGATCTTTTGCTTGCAGGAT-3’. The GAPDH primers were purchased from Shanghai Bioengineering Company.

The silencing RNA against AGAP2-AS1 (si-AGAP2-AS1) and hnRNPA2B1 (si-hnRNPA2B1) were purchased from RiboBio (Guangzhou, China). Negative control siRNA was purchased from Invitrogen (CAT#12935-110, Shanghai, China). Green fluorescence protein (GFP) was used to show transfection efficiency. Cell lines were transfected using Lipofectamine 2000 (Life Technologies, USA) at the concentration of 100 nM, according to the manufacturer’s instructions. The sequences of small interfering RNAs were: si-AGAP2-AS1#1 5′-CCACTCCACCTCAAACTCTTACCTT-3′; si-AGAP2-AS1#2 5′-GGGTCATTAAGGGACAGAGTTCAAG-3′; si-AGAP2-AS1#3 5′-CAGGTGGACTCACAATTCCAAATAT-3′; si-hnRNPA2B1 5′-GCGGAAUUAAAGAAGAUACTT-3′.