Zorbax sb

Manufactured by Agilent Technologies

Sourced in United States

Zorbax SB is a high-performance liquid chromatography (HPLC) column manufactured by Agilent Technologies. It is designed for the separation and analysis of a wide range of chemical compounds. The column utilizes a silica-based stationary phase with a proprietary surface chemistry to provide efficient and reproducible separations.

Automatically generated - may contain errors

Lab products found in correlation

Ltq velos, by Thermo Fisher Scientific (2 mentions) Avance 400, by Bruker (1 mentions) Zorbax eclipse xbd c18, by Agilent Technologies (1 mentions) Easy nanolc 2 hplc system, by Thermo Fisher Scientific (1 mentions) Xevo g2 xs qtof, by Waters Corporation (1 mentions) High performance liquid chromatography (hplc), by Shimadzu (1 mentions) Waters 2695 hplc, by Waters Corporation (1 mentions) Waters 2489 uv vis detector, by Waters Corporation (1 mentions) 1100 nano flow system, by Agilent Technologies (1 mentions)

9 protocols using zorbax sb

HPLC Optimization for Dp3S Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

During the initial method development, different mobile phase compositions, columns, flow rates, detection wavelength, and oven temperatures were tested to achieve the best separation of Dp3S peak from the other peaks of the excipients, degradants, and impurities with good resolution, sharpness, and symmetry. Moreover, to get the separation with shortest sample run and retention times with cost-effective and easy mobile phase preparation.
In the preliminary studies, several mobile phase systems used different combinations of methanol or ACN as organic phase and pure or acidified water with acetic, FA, or phosphoric acids as the aqueous phase at various levels ratios were evaluated. Three different C18 columns including: (Waters Symmetry® W, 5 μm, 4.6 × 150 mm, Agilent Zorbax® SB, 5 μm, 4.6 × 250 mm and Agilent Eclipse plus®, 5 μm, 4.6 × 250 mm), were put into trials as stationary phases for proper separation and determination of Dp3S peak. Furthermore, different levels of the flow rate (0.6–1 mL/min), the injection volume (5–20 μl), the detection wavelengths (520–530 nm), and oven temperatures (30–45 °C) were also investigated.

Khan N.H., Abdulbaqi I.M., Darwis Y., Aminu N, & Chan S.Y. (2022). A stability-indicating HPLC-UV method for the quantification of anthocyanin in Roselle (Hibiscus Sabdariffa L.) spray-dried extract, oral powder, and lozenges. Heliyon, 8(3), e09177.

+ Open protocol

+ Expand

NMR and LC-QTOF-MS Analysis of Fractions

Check if the same lab product or an alternative is used in the 5 most similar protocols

The fractions were analyzed by NMR experiments, including ¹H and ¹³C NMR (Bruker Avance 400, 400 MHz for ¹H and 100 MHz for ¹³C) using CDCl₃ as solvent. The fractions were analyzed with an Agilent 6520 Accurate-Mass Quadrupole Time-of-Flight LC-QTOF-MS System (Agilent Technologies, Palo Alto, USA) with a reversed phase C18 analytical column (Agilent, Zorbax SB, 5 μm - 4.6 × 150 mm) using a gradient solvent system with A (water and 0.1% trifluoroacetic acid) and B (acetonitrile and 0.1% trifluoroacetic acid), from 20% B at zero minutes to 40% B in 4 min, to 70% B in 10 min and then to 90% B in 27 min. Five microliters of a 1 mg/mL solution of crude extracts were injected and analyzed at a flowrate of 0.5 mL/min in positive ion mode. The instrument was externally calibrated before the runs and the selected mass range was 100–3000 m/z.

Conde-Martínez N., Acosta-González A., Díaz L.E, & Tello E. (2017). Use of a mixed culture strategy to isolate halophilic bacteria with antibacterial and cytotoxic activity from the Manaure solar saltern in Colombia. BMC Microbiology, 17, 230.

+ Open protocol

+ Expand

Quantitative Analysis of Carnosol, Carnosic, and Rosmarinic Acids

Check if the same lab product or an alternative is used in the 5 most similar protocols

Carnosol, carnosic, and rosmarinic acids were quantitatively analyzed using an HPLC system (Agilent 1100, Les Ulis, France) equipped with a photo diode array detector (DAD) according to our internal method developed [9 (link)], whose procedures are detailed below. Carnosol and carnosic acid were detected at a wavelength of 230 nm using a C18 column (1.8 µm, 4.6 mm × 50 mm, Zorbax Eclipse XBD-C18, Agilent Technologies, Courtaboeuf, France). The mobile phase was isocratic and composed of 0.5% phosphoric acid in water:acetonitrile (35:65, v/v). Samples (5 µL) were injected with a flow rate of 1.5 mL/min and the column oven temperature was 25 °C. Rosmarinic acid was detected at a wavelength of 328 nm using a C18 column (5 µm, 4.6 mm × 250 mm, Zorbax SB, Agilent Technologies, Courtaboeuf, France). The mobile phase composed of acetonitrile (32%) and 0.1% of trifluoroacetic acid in water (68%). Samples (5 µL) were injected with a flow rate of 1 mL/min and the column oven temperature was 20 °C with a 10 min runtime.

Li Y., Bundeesomchok K., Rakotomanomana N., Fabiano-Tixier A.S., Bott R., Wang Y, & Chemat F. (2019). Towards a Zero-Waste Biorefinery Using Edible Oils as Solvents for the Green Extraction of Volatile and Non-Volatile Bioactive Compounds from Rosemary. Antioxidants, 8(5), 140.

+ Open protocol

+ Expand

Liquid Chromatography-Mass Spectrometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Each reaction mixture was analysed by LC–MS/MS. LC was performed on a Easy nanoLC II HPLC system (Thermo Fisher Scientific). Mobile phase A was 94.5% MilliQ water, 5% acetonitrile, 0.5% acetic acid. Mobile phase B was 80% acetonitrile, 19.5% MilliQ water, 0.5% acetic acid. The 120-min LC gradient ran from 2% B to 35% B over 90 min, with the remaining time used for sample loading and column regeneration. Samples were loaded to a 2 cm × 100 μm I.D. trap column positioned on an actuated valve (Rheodyne). The column was 13 cm × 100 μm I.D. fused silica with a pulled tip emitter. Both trap and analytical columns were packed with 3.5 µm C18 resin (Zorbax SB, Agilent). The LC was interfaced to a dual pressure linear ion-trap mass spectrometer (LTQ Velos, Thermo Fisher) via nano-electrospray ionisation. An electrospray voltage of 1.8 kV was applied to a pre-column tee. The mass spectrometer was programmed to acquire, by data-dependent acquisition, tandem mass spectra from the top 15 ions in the full scan from 400 to 1400 m/z. Dynamic exclusion was set to 30 s.

Mahadevan I.A., Pentakota S., Roy R., Bhaduri U, & Satyanarayana Rao M.R. (2019). TH2BS11ph histone mark is enriched in the unsynapsed axes of the XY body and predominantly associates with H3K4me3-containing genomic regions in mammalian spermatocytes. Epigenetics & Chromatin, 12, 53.

+ Open protocol

+ Expand

Quantitative Analysis of Target Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

The collected samples were centrifuged at 3,000 r/min for 15 min, and the supernatant was taken for freeze-drying. The freeze-dried product was redissolved with 2 ml methanol, and filtered by a 0.22-μm filter. Following that, 20 μl filtrate was taken for qualitative and quantitative analysis by high-performance liquid chromatography (HPLC, Shimadzu, Japan) equipped with a UV detector and a C18 column (4.6 × 250 mm², 5 μm, ZORBAX SB, Agilent, USA). The molecular weight of the target products in the filtrate was analyzed by a high-resolution mass spectrometer (Xevo G2-XS QTof, Waters, USA).
For HPLC analysis, the mobile phase consisted of 25% methanol and 75% water. The column temperature was kept at 40°C, and the constant flow rate was 1 ml/min. The target products were detected at 276 nm.
For mass spectrometry, the detection parameters were set as follows: positive ion mode; drying gas flow rate of 6 l/min; nebulizer pressure of 40 Psig; atomization gas temperature at 325°C; sheath gas temperature at 350°C; sheath gas flow rate of 12 l/min; capillary voltage of 4000 V; mass spectrum acquisition range between 50 and 1000 m/z.

Wu W., Yuan X., Gao X., Tan C., Li S, & Xu D. (2023). Production of ρ-Hydroxyacetophenone by Engineered Escherichia coli Heterologously Expressing 1-(4-Hydroxyphenyl)-Ethanol Dehydrogenase. Journal of Microbiology and Biotechnology, 34(2), 467-475.

+ Open protocol

+ Expand

HPLC Analysis of Grape and Wine Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

The grape juice (GJ), condensed juice (CJ), Msalais wine (MW), and aged Msalais wine (AW) were centrifuged at 3000× g for 15 min at 4 °C. The supernatant was filtered through a 0.45 μm PTFE membrane filter for chromatographic analysis.
HPLC analysis was carried out using Waters 2695 HPLC coupled to a Waters 2489 UV–Vis detector (Waters Corp., Milford, MA, USA). A C18 type column (Agilent ZORBAX SB, 250 × 4.6 mm, i.e., 5 µm) was used at 25 °C for the separation of compounds. The mobile phases consisted of 0.1% formic acid solution (eluent A), methanol (eluent B), and acetonitrile (eluent C). A 10 μL sample was injected, and the flow rate was adjusted to 1 mL/min. The gradient program was as follows: 0–6 min: 85–59% A, 10–36% B; 6–30 min: 59–54% A, 36–44% B. The detection wavelengths were 280 nm and 360 nm for the real-time monitoring of the peak intensity.

Hou X., Chen S., Pu Y., Wang T., Xu H., Li H., Ma P, & Hou X. (2023). Effect of Winemaking on Phenolic Compounds and Antioxidant Activities of Msalais Wine. Molecules, 28(3), 1250.

+ Open protocol

+ Expand

LC-MS/MS Analysis of SAX Fractions

Check if the same lab product or an alternative is used in the 5 most similar protocols

Each SAX fraction was analyzed by LC-MS/MS. LC was performed on an Agilent 1100 Nano-flow system. Mobile phase A was 94.5% MilliQ water, 5% acetonitrile, 0.5% acetic acid. Mobile phase B was 80% acetonitrile, 19.5% MilliQ water, 0.5% acetic acid. The 150 min LC gradient ran from 5% A to 35% B over 105 min, with the remaining time used for sample loading and column regeneration. Samples were loaded to a 2 cm x 100 um I.D. trap column positioned on an actuated valve (Rheodyne). The column was 13 cm x 100 μm I.D. fused silica with a pulled tip emitter. Both trap and analytical columns were packed with 3.5 um C18 (Zorbax SB, Agilent). The LC was interfaced to a dual pressure linear ion trap mass spectrometer (LTQ Velos, Thermo Fisher) via nano- electrospray ionization. An electrospray voltage of 1.5 kV was applied to a pre-column tee. The mass spectrometer was programmed to acquire, by data-dependent acquisition, tandem mass spectra from the top 15 ions in the full scan from 400–1400 m/z. Dynamic exclusion was set to 30 s.

Velez G., Machlab D.A., Tang P.H., Sun Y., Tsang S.H., Bassuk A.G, & Mahajan V.B. (2018). Proteomic analysis of the human retina reveals region-specific susceptibilities to metabolic- and oxidative stress-related diseases. PLoS ONE, 13(2), e0193250.

+ Open protocol

+ Expand

Quantification of Rosmarinic Acid by HPLC

Check if the same lab product or an alternative is used in the 5 most similar protocols

The column used was a C₁₈ column (5 µm, 4.6 mm × 250 mm, Zorbax SB, Agilent Technologies, Santa Clara, CA, USA). The mobile phase was composed of 32% acetonitrile and 68% water with 0.1% trifluoroacetic acid (mL/mL) and the flow rate was set at 1 mL/min. The column oven temperature was 20 °C and the run time was 10 min. Five µL were injected. Rosmarinic acid was detected at a wavelength of 328 nm. For quantification of rosmarinic acid in the extract, a calibration curve was calculated by linear regression analysis for rosmarinic acid standard.

Hirondart M., Rombaut N., Fabiano-Tixier A.S., Bily A, & Chemat F. (2020). Comparison between Pressurized Liquid Extraction and Conventional Soxhlet Extraction for Rosemary Antioxidants, Yield, Composition, and Environmental Footprint. Foods, 9(5), 584.

+ Open protocol

+ Expand

HPLC Chemical Profiling of GJD Extract

Check if the same lab product or an alternative is used in the 5 most similar protocols

The chemical pro le of GJD aqueous extract was determined by high-performance lipid chromatography (HPLC). Standard chemicals (Paeoni orin, Pinoresinol diglucoside, Loganin, Liquiritin, Acteoside, Tetrahydropalmatine, Naringin, Icariin, Ammonium glycyrrhetate and Catalpol) were purchased from National Institute for Food and Drug Control (Beijing, China). HPLC analysis of GJD extracts was carried out using a liquid chromatography system ZORBAX SB (250 mm × 4.6 mm, 5 μm) (Agilent, USA). The mobile phase was a gradient elution system consisting of acetonitrile (A) -0.1% phosphoric acid water (B) at a ow rate of 1 ml/min as follows: 0-8 min, 7%-15%A, 8-25 min, 15%-20%A, 25-45 min, 20%-35%A, 45-60 min, 35%-45%A, 60-80 min, 45%-55%A, 80-110 min, 55%A, 80~110 min, 55%~60%A, 110~120 min, 60%~70%A, 120~140 min, 70%~80%A. A photodiode array (PDA) detector was set at 254 nm.

Zhou Y., Liu C., Zhou Z., Li X., Su S., Ng L., Zhong J., Wang T., Yang J., Peng Z., Zhang S., & Yao B. (2021). Gujin Dan is A Chinese Medicine Formulation That Stimulates Cell Proliferation And Differentiation By Controlling Multiple Genes Involved In MC3T3-E1 Cells.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!