Verikine hs human ifn alpha all subtype elisa kit

Serum cytokines and chemokines (IFN-α, IFN-β, IL-6, interleukin-10 (IL-10), and CXCL10) were measured using commercially available ELISA assays, according to the manufacturers’ instructions. The levels of IFN-α, IFN-β, IL-6, IL-10, and CXCL10 were measured using the VeriKine-HS Human IFN Alpha All Subtype ELISA Kit (PBL Assay Science, New Jersey, USA), the VeriKine-HS Human IFN Beta Serum ELISA Kit (PBL Assay Science), the AuthentiKine™ Human IL-6 ELISA Kit (Proteintech, Illinois, USA), the AuthentiKine™ Human IL-10 ELISA Kit (Proteintech), and the Human CXCL10/IP-10 ELISA Kit (Proteintech), respectively. Each sample was measured on first thaw. If an analyte signal was below the background signal, it was set to zero, and if the signal was detectable, but below the manufacturer’s lower limit of quantification, it was set to the lower limit of detection.

Serum cytokines and chemokines (IFN-α, IL-6, CXCL10, and VEGF) were measured using commercially available enzyme-linked immunosorbent assay (ELISA) kits, according to the manufacturers’ instructions. IFN-α levels were measured using the VeriKine-HS Human IFN Alpha All Subtype ELISA Kit (PBL Assay Science, Piscataway, NJ, USA), CXCL10 levels were measured using the Human CXCL10/IP-10 ELISA Kit (Proteintech, Rosemont, IL, USA), IL-6 levels were measured using the AuthentiKine™ Human IL-6 ELISA Kit (Proteintech, Rosemont, IL, USA), and VEGF levels were measured using the AuthentiKine™ Human VEGF ELISA Kit (Proteintech, Rosemont, IL, USA). Each sample was measured at first sight. If an analyte signal was below the background signal, it was set to zero; if the signal was detectable but below the manufacturer’s lower limit of quantification, it was set to the lower limit of detection.