Jc 1 cationic fluorescent dye kit

Manufactured by Merck Group

The JC-1 cationic fluorescent dye kit is a laboratory reagent used for the detection and measurement of mitochondrial membrane potential in cells. The kit contains the JC-1 dye, which exhibits potential-dependent accumulation in mitochondria, indicated by a fluorescence emission shift from green to red as the membrane potential increases.

Automatically generated - may contain errors

Lab products found in correlation

Aposensor assay kit, by Abcam (1 mentions) Mitoprobe transition pore assay kit, by Thermo Fisher Scientific (1 mentions) Aposensor adp atp ratio bioluminescent assay kit, by Abcam (1 mentions)

Close spelling variants of this product

JC-1 dye JC-1 kit Fluorescent dyes

2 protocols using jc 1 cationic fluorescent dye kit

Mitochondrial Function Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Isolated mitochondria were used to determine several measures of mitochondrial function as previously described.16 The measures included (i) ADP‐dependent mitochondrial respiration as previously described using a Strathklein respirometer; (ii) maximum rate of ATP synthesis (ATPsyn) using the bioluminescent ApoSENSOR assay kit (BioVision, Milpitas, CA)26; (iii) cytochrome c oxidase (COX‐IV) activity measured polarographically; (iv) mitochondrial membrane potential (Δψm) measured using the commercially available JC‐1 cationic fluorescent dye kit (Sigma, St. Louis, MO)26; and (v) mitochondrial permeability transition pore (mPTP) opening assessed using a MitoProbe Transition Pore assay kit (Molecular Probes, Eugene, OR, USA Cat# M34153).26

Sabbah H.N., Gupta R.C., Singh‐Gupta V, & Zhang K. (2019). Effects of elamipretide on skeletal muscle in dogs with experimentally induced heart failure. ESC Heart Failure, 6(2), 328-335.

+ Open protocol

+ Expand

Comprehensive Mitochondrial Function Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following MITO function measures were assessed in freshly, collagenase-isolated, digitonin-permeabilized cardiomyocytes: 1) MITO state-3 respiration was measured using a Clark-type electrode (Strathkelvin Respirometer), 2) MITO membrane potential (Δψm) was measured using the commercially available JC-1 cationic fluorescent dye kit (Sigma, St. Louis, MO), 3) MITO permeability transition pore (mPTP) opening was assessed using calcein and 4) MITO maximal rate of ATP synthesis was determined using the ApoSENSOR™ ADP/ATP ratio bioluminescent assay kit (BioVision, Milpitas, CA) as previously described (7 ). Mitochondrial Complex-I and complex-IV activities were determined in MITO fractions as previously described (7 ).

Sabbah H.N., Zhang K., Gupta R.C., J.i.a.n.g.-.X.u, & Singh-Gupta V. (2020). Effects of Angiotensin-Neprilysin Inhibition in Canines with Experimentally-Induced Cardiorenal Syndrome. Journal of cardiac failure, 26(11), 987-997.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!