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2 protocols using 4 dimethylaminopyridine dmap

1

Synthesis of Fluorescent Poly(2-Ethyl-2-Oxazoline)

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Methyl p-toluenesulfonate, ethylenediamine, and super dehydrated acetonitrile were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). 2-Ethyl-2-oxazoline was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (WSC) was purchased from Dojindo Molecular Technologies, Inc. (Kumamoto, Japan). 4-Dimethylaminopyridine (DMAP) and 2 mol/l-hydrochloric acid were purchased from Nacalai Tesque, Inc. (Kyoto, Japan). Poly(2-Ethyl-2-oxazoline) (molecular weight: 25, 50, and 200 kDa) was purchased from Sigma-Aldrich Japan (Tokyo, Japan). 1H-Benz[e]indolium, 2-[7-[3-(5-carboxypentyl)-1,3-dihydro-1,1-dimethyl-2H-benz[e]indol-2-ylidene]-1,3,5-heptatrien-1-yl]-1,1-dimethyl-3-(4-sulfobutyl), inner salt (ICCA) was synthesized according to previously reported method32 (link). All other chemicals of highest purity available were used.
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2

Synthesis and Characterization of Multifunctional Polymer Conjugates

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Tetra‐poly(ethyl glycol)‐amine (Sunbright PTE‐050PA; Mn, 5328 g/mol) was purchased from NOF Corporation (Tokyo, Japan). N,N,N′,N′‐tetramethylethylenediamine (TEMED), dichloromethane, acryloyl chloride, triethylamine, ammonium persulfate (APS), hydrochloric acid, methanol, and diethyl ether were purchased from Wako Pure Chemical Industries (Osaka, Japan). Dimethyl sulfoxide, 2‐morpholineoethanesulfonic acid and 4‐dimethylaminopyridine (DMAP) were obtained from Nacalai Tesque (Kyoto, Japan). Dextran with a weight‐averaged molecular weight of 40 000 (Dex), diethylenetriaminepentaacetic acid (DTPA) anhydride, manganese chloride, first generation polypropylenimine tetramine dendrimer (DAB‐Am‐4), and rhodamine 6G were purchased from Sigma‐Aldrich (St. Louis, Missouri). Water was purified with a Milli‐Q apparatus (Millipore, Bedford, Massachusetts). A detailed materials preparation protocol has been published previously.16, 17, 18
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