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4 protocols using delphinidin 3 glucoside

1

Antioxidant Capacity Analysis Protocol

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The analytical-grade reagents (formic acid, acetonitrile, methanol and water), as well as sodium carbonate (Na2CO3), potassium chloride, the radical 2,2-diphenyl-1-picrylhydracil (DPPH), Trolox, FeCl3-6H2O, HCl and TPTZ were obtained from Merck (Darmstadt, Germany). Commercial standards of delphinidin 3-glucoside (98.84%) and cyanidin 3-glucoside (99.21%) were obtained from Sigma Aldrich (St. Louis, MO, USA).
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2

Antioxidant and Acetylcholinesterase Inhibition Assessment

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Maltodextrin DE-10, as a carrier agent, was purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Analytical grade reagents such as formic acid, acetonitrile, methanol, water, monobasic sodium phosphate, dibasic sodium phosphate, and ethanol were acquired from Merck (Darmstadt, Germany). Commercial standards of delphinidin 3-glucoside (95%), cyanidin 3-glucoside (98%) chlorogenic acid, rutin trihydrate, quercetin dihydrate, radical 2,2-diphenyl-1-picrylhydracil (DPPH*), Trolox, fluorescein (free acid), and 2,2-azobis-(-2-methylpropionamidine) dihydrochloride (AAPH) were acquired from Sigma Aldrich (St. Louis, MO, USA). For the enzymatic assays, E. electricus AChE, acetylthiocholine iodide (ATCh), and 5,5′-dithio bis(2 nitrobenzoic) acid (DTNB) were supplied by Sigma-Aldrich Chemical Co. (St. Louis, MO, USA).
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3

Antioxidant Profile of Red Onion Cultivars

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Honeysuckle red onions from J & D Produce, Inc., Texas and Artisan sweet Italian red onions from Tanimura & Antle, California were used for the entire study (Figure 1). Sodium pyruvate, 2, 4-dinitrophenylhydrazine (DNPH), glucose, sucrose, fructose, quercetin 3-glucoside, quercetin-3,4′-diglucoside, quercetin, delphinidin 3-glucoside, L-ascorbic acid, gallic acid, dextrose, sodium carbonate, 2,2-diphenyl-1-picryhydrazyl, 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid), Folin-Ciocalteu reagent, HPLC grade methanol, acetonitrile, formic acid, and metaphosphoric acid were purchased from Sigma Aldrich (St. Louis, MO, USA). Sodium hydroxide (NaOH) was obtained from Fisher Scientific (Pittsburg, PA, USA).
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4

Anthocyanin Extraction and Analysis

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Acetonitrile, methanol, formic acid, hydrochloric acid, water (HPLC grade), MK-571, Amberlite XAD7-HP, anthocyanin standards (pelargonidin-3 glucoside, pelargonidin-3-rhamnosylglucoside, cyanidin-3-glucoside, delphinidin-3- glucoside, malvidin-3-glucoside, petunidin-3-glucoside, peonidin-3-glucoside), 4’,6-diamidino-2-phenylindole (DAPI) were purchased from Sigma-Aldrich, Gillingham, UK. Caco-2 cells were purchased from European Collection of Authenticated Cell Cultures (ECACC), Salisbury, UK. Concentrate of Strawberry was purchased from Cobell, Exeter, UK and red grape concentrate from Homebrew shop, Aldershot, UK. The other reagents were from Lonza, Basle, Switzerland.
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