The chemicals used in this research were commercially obtained: simvastatin (>97%, Sigma Aldrich Inc., USA), rosuvastatin calcium (>98%, Sigma Aldrich Inc., USA), fluvastatin sodium hydrate (>98%, Sigma Aldrich Inc., USA, Cas number 93957–55-2), atorvastatin calcium salt trihydrate (>98%, Sigma Aldrich Inc., Cas number 344423–98-9) and pravastatin sodium salt hydrate (>98%, Sigma Aldrich Inc., Cas number 81131–70-6).
Atorvastatin calcium salt trihydrate
Manufactured by Merck Group
Sourced in United States
Atorvastatin calcium salt trihydrate is a white to off-white crystalline powder. It is a synthetic lipid-lowering agent used as a reference standard in the analysis and testing of pharmaceutical products.
Automatically generated - may contain errors
Lab products found in correlation
Simvastatin, by Merck Group (4 mentions)
Rosuvastatin calcium, by Merck Group (2 mentions)
Fluvastatin sodium hydrate, by Merck Group (2 mentions)
Pravastatin sodium salt hydrate, by Merck Group (2 mentions)
Cerivastatin sodium salt hydrate, by Merck Group (2 mentions)
Lipopolysaccharide, by Merck Group (1 mentions)
Isoproterenol, by Pfizer (1 mentions)
Skbr3, by American Type Culture Collection (1 mentions)
Bt474, by American Type Culture Collection (1 mentions)
Mda mb 231, by American Type Culture Collection (1 mentions)
Mcf 7, by American Type Culture Collection (1 mentions)
Xcelligence real time cell analysis instrument, by Agilent Technologies (1 mentions)
Atorvastatin, by Agilent Technologies (1 mentions)
Ggti 298, by Merck Group (1 mentions)
Mevalonolactone, by Merck Group (1 mentions)
Pravastatin, by Merck Group (1 mentions)
Geranylgeranyl pyrophosphate ammonium salt, by Merck Group (1 mentions)
Pitavastatin, by Selleck Chemicals (1 mentions)
Fluvastatin, by LGC (1 mentions)
Geranyl pyrophosphate, by Merck Group (1 mentions)
8 protocols using atorvastatin calcium salt trihydrate
1
Commercially Obtained Statin Compounds
2
Isoproterenol Stimulation of Ana-1 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Isoproterenol was from Hospira (catalog 0409-1410-05, Hospira, UT, USA). Lipopolysaccharide (catalog number L2630) and atorvastatin calcium salt trihydrate (134523-03-8) were from Sigma Aldrich. The Ana-1 cell line was from the Cell Bank of the Chinese Academy of Sciences. Transwells were purchased from Corning Costar Company (catalog number 3422, Corning Costar, Cambridge, MA, USA).
3
Evaluating Atorvastatin's Impact on Breast Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human breast cancer (BC) cell lines [T47D, MCF7, BT474, SKBR3 and MDA-MB-231] were purchased from ATCC (Rockville, MD) and asynchronously grown at 37 °C and 5% CO2 humidified atmosphere. T47D cells were maintained in RPMI 1640 medium and the remaining cell-lines were grown in culture media as described previously [10], supplemented with 10% (v/v) fetal bovine serum (FBS-HyCloneTM) and with an antibiotic cocktail made of 100 U/ml penicillin and 100 mg/ml streptomycin. atorvastatin calcium salt trihydrate (Sigma Aldrich) was dissolved in DMSO prior to cell treatments. To determine sensitivity, BC cell lines were exposed to increasing concentrations of atorvastatin (1–50 μM) for 72 hrs and the effects on cell growth were assessed by using xCelligence Real Time Cell Analysis (RTCA) instrument (ACEA Bioscience, Inc).
4
Statin Drugs and Cholesterol Synthesis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Nine statin drugs were obtained from commercial sources: Atorvastatin calcium salt trihydrate, simvastatin, pravastatin, mevastatin (PZ0001, S6196, P4498, M2537 Sigma, St Louis, MO, USA); fluvastatin, rosuvastatin, cerivastatin (F601250, R700500, C277000, Toronto Research Chemicals Inc., Toronto, ON, Canada); pitavastatin (S1759, Selleckchem, Boston, MA, USA); lovastatin (430-103-M050, Enzo life sciences, Farmingdale, NY, USA). We prepared 10 mM stock solution in DMSO for in vitro testing. For in vivo testing, pitavastatin was prepared as fresh suspension in methycellulose at 0.4 mg ml−1 (for oral gavage). fluvastatin, cerivastatin, and pitavastatin were prepared as a solution at 0.25 mg ml−1 in PBS for IP injection. GGTI-298 and FTI-277 (G5169, A1393, Sigma) were made into 10 mM stock solution in DMSO. AICAR (#S1802, Selleckchem) was made into 100 mM stock solution in PBS. Intermediate products of cholesterol synthesis were purchased from Sigma: mevalonolactone (M4667), farnesyl pyrophosphate ammonium salt (F6892), geranylgeranyl pyrophosphate ammonium salt (G6025), squalene (S3626), cholesterol solution (S5442), geranylpyrophosphate (G6772), and isopentenyl pyrophosphate triammonium salt solution (I0503).
5
Multicomponent Pharmaceutical Compound Synthesis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
FK506 (catalog no. 10007965) was purchased from Cayman Chemical. Methotrexate (catalog no. M4010), doxorubicin (catalog no. D1515), d ‐sorbitol (catalog no. S1876), l ‐methionine (catalog no. M‐9625), copper(II) sulfate (catalog no. 451657), sodium chloride (catalog no. S7653), anhydrotetracycline hydrochloride (catalog no. 37919), hydrogen peroxide solution (catalog no. 216763), atorvastatin calcium salt trihydrate (catalog no. PZ0001) were purchased from Sigma‐Aldrich. A full description of environmental conditions is listed in Dataset EV1 .
6
Statin and Azacytidine Dissolution Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Atorvastatin calcium salt trihydrate (CAS No. 134523038), cerivastatin sodium salt hydrate (CAS No. 143201110), rosuvastatin calcium salt (CAS No. 147098202), simvastatin (CAS No. 79902639), and 5-azacytidine (CAS No. 320672) were purchased from Sigma Aldrich, USA. All drugs dissolved in DMSO (Sigma Aldrich, St. Louis, MO, USA).
7
Statin and Chloroquine Antiviral Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Atorvastatin calcium salt trihydrate (ATO), cerivastatin sodium salt hydrate (CER), fluvastatin sodium hydrate (FLU), pravastatin sodium salt hydrate (PRA), rosuvastatin calcium (ROS), simvastatin (SIM), and chloroquine (CHL) were purchased from Sigma-Aldrich. Lovastatin sodium salt (LOV) and mevastatin sodium salt (MEV) were purchased from Calbiochem. The statins ATO, FLU, PRA, ROS, MEV, LOV, and SIM were reconstituted to 20 mM in dimethyl sulfoxide (DMSO); PRA was reconstituted to 50 mM in DMSO; CER was reconstituted to 20 mM in distilled deionized water. All statins were stored at −20 °C and diluted to working concentrations in infection medium on the same day of use. CHL was dissolved to 100 mM in distilled deionized water, stored at 4 °C, and diluted to the required concentration on the same day of use.
Mouse anti-flavivirus group antigen monoclonal antibody clone D1-4G2-4-15 (4G2 mAb) was purchased from Merck-Millipore (MAB10216) and stored at −20 °C in aliquots prior to use. The secondary antibody, fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse IgG, was purchased from Sigma-Aldrich. The cytotoxicity assay kit, EZ-CYTOX, was purchased from DoGenBio, Co., Ltd. in South Korea.
Mouse anti-flavivirus group antigen monoclonal antibody clone D1-4G2-4-15 (4G2 mAb) was purchased from Merck-Millipore (MAB10216) and stored at −20 °C in aliquots prior to use. The secondary antibody, fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse IgG, was purchased from Sigma-Aldrich. The cytotoxicity assay kit, EZ-CYTOX, was purchased from DoGenBio, Co., Ltd. in South Korea.
8
Cholesterol and 25-OH Complexation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
TNF was purchased from R&D Systems (Minneapolis, MN). Atorvastatin calcium salt trihydrate, simvastatin (lactone), cholesterol, methyl-betacyclodextrin and T0901317 were from Sigma Aldrich (St. Louis, MO). Unless otherwise specified, all other chemicals used in this study were purchased from Sigma Aldrich (St. Louis, MO). 25-hydroxycholesterol (25-OH) was purchased from Avanti Polar Lipids. Cholesterol and 25-OH were complexed to cyclodextrin as described in Yang and Gelissen [20] .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!