The human breast cancer cell lines, MCF-7 (HER-2 negative), MDA-MB-453 (HER-2 positive), and the normal breast cell line, MCF-10A, were purchased from the Chinese Academy of Science. The MCF-7 cells were cultured in DMEM/high glucose with 10% FBS, and the MCF-10A cells were cultured in DMEM/F12 supplemented with 6% horse serum, 10 µg/ml insulin (Wako, Osaka, Japan), 20 ng/ml epidermal growth factor (PeproTech, Rocky Hill, NJ, USA), 100 ng/ml cholera toxin and 0.5 µg/ml hydrocortisone in humidified 5% CO2 at 37°C. The MDA-MB-453 cells were cultured in L15 medium with 10% FBS in 100% O2 at 37°C.
Mcf 7
Manufactured by Fujifilm
Sourced in Japan
The MCF-7 is a cell line derived from human breast adenocarcinoma. It is widely used in cancer research and drug development as a model for studying the biology and treatment of breast cancer.
Automatically generated - may contain errors
Lab products found in correlation
Mcf 7, by American Type Culture Collection (4 mentions)
Rpmi 1640, by Fujifilm (3 mentions)
Epidermal growth factor (egf), by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Fujifilm (2 mentions)
Dulbecco modified eagle medium (dmem), by Fujifilm (2 mentions)
Mda mb 231, by American Type Culture Collection (2 mentions)
Insulin, by Fujifilm (1 mentions)
Mcf 10a, by Fujifilm (1 mentions)
Sodium pyruvate solution, by Fujifilm (1 mentions)
Fetal bovine serum (fbs), by GE Healthcare (1 mentions)
Mem non essential amino acid solution, by Thermo Fisher Scientific (1 mentions)
Mda mb 453, by RIKEN Cell Bank (1 mentions)
Horse serum, by Thermo Fisher Scientific (1 mentions)
Dextran coated charcoal stripped fbs, by Thermo Fisher Scientific (1 mentions)
Mcf 10a, by American Type Culture Collection (1 mentions)
Insulin, by Merck Group (1 mentions)
Hydrocortisone, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Corning (1 mentions)
Rpmi 1640, by Corning (1 mentions)
Dmem medium, by Fujifilm (1 mentions)
8 protocols using mcf 7
1
Culturing Breast Cell Lines for Research
2
Hyperthermia treatment of breast cancer cells
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The estrogen receptor (ER)-positive human breast cancer line MCF-7 (RCB1904) and the ER-negative human breast cancer cell line MDA-MB-453 (RCB1192) were purchased from the RIKEN cell bank. MCF-7 cells were cultured at 37 °C with 5% CO2 in E-MEM with L-glutamine and phenol red (Wako Pure Chemical Industries, Ltd., Osaka, Japan) containing 10% fetal bovine serum (GE Healthcare Life Sciences, Inc., Logan, UT, USA), 1% penicillin/streptomycin (Wako Pure Chemical Industries, Ltd.), 1.0 mM sodium pyruvate solution (Wako Pure Chemical Industries, Ltd.), and 1% MEM non-essential amino acid solution (Thermo Fisher Scientific, Inc., Waltham, MA, USA). MDA-MB-453 cells were cultured at 37 °C without CO2 in Leibovitz′s L-15 Medium with L-glutamine, phenol red, and sodium pyruvate (Wako Pure Chemical Industries, Ltd.) containing 10% fetal bovine serum (GE Healthcare Life Sciences, Inc.) and 1% penicillin/streptomycin (Wako Pure Chemical Industries, Ltd.).
The hyperthermia treatment was based on our previous study [11 (link)]. The MCF-7 cells were incubated at 42 °C with 5% CO2 for 1 h using a standard incubator, and the MDA-MB-453 cells were incubated in the same way, but without CO2.
The hyperthermia treatment was based on our previous study [11 (link)]. The MCF-7 cells were incubated at 42 °C with 5% CO2 for 1 h using a standard incubator, and the MDA-MB-453 cells were incubated in the same way, but without CO2.
3
Culturing of Breast Cell Lines
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The human immortalized normal breast cell line, MCF10A (ATCC), was cultured in DMEM/F12 with 5% horse serum (Gibco), 0.5 μg/ml hydrocortisone (Sigma), 100 ng/ml cholera toxin (Bio Academia), 10 μg/ml insulin (Sigma), and 20 ng/ml epidermal growth factor (PeproTech). The human estrogen receptor-positive breast cancer cell line, MCF7 (ATCC), was grown in RPMI 1640 containing 10% FBS (Corning). LTED cells were established by culturing MCF7 cells in phenol red-free RPMI 1640 (Wako), containing 4% dextran-coated charcoal-stripped FBS (Thermo Fisher Science), for 3–8 months. The cells were cultured at 37 °C with a humidified 5% CO2 atmosphere.
4
Cell Culture Conditions for Cancer Cell Lines
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HLC-1 cells were purchased from Riken Cell Bank (Saitama, Japan). NCI-H2347, NCI-H1975 and MCF-7 cells were purchased from American Type Culture Collection (Manassas, VA, USA). HLC-1 cells were grown in Ham's F12 medium (087–08335; Wako Pure Chemical Industries, Ltd., Osaka, Japan). NCI-H2347 and NCI-H1975 cells were grown in RPMI-1640 medium (R8758; Sigma-Aldrich; Merck KGaA, Darmstadt, Germany), MCF-7 cells were grown in DMEM medium (Wako Pure Chemical Industries, Ltd.). All media contained 10% fetal bovine serum (Nichirei Biosciences, Tokyo, Japan) and 1% penicillin-streptomycin (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany). Cells were cultured at 37°C in a humidified atmosphere of 5% CO2.
5
Culturing Breast Cancer and Kidney Cell Lines
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Human embryonic kidney cells 293 T and human breast cancer cell lines MCF-7 and MDA-MB231 were purchased from ATCC, and human breast cancer cell lines SKBR3 and BT474 were gifted by Dr. S. Hayashi (Tohoku University, Miyagi, Japan). The cells were cultured in DMEM (Wako) or RPMI 1640 (Wako) supplemented with 10% heat-inactivated FBS (Biowest), 100 units/ml penicillin G and 100 µg/ml streptomycin. For experiments evaluating the effect of 17β-estradiol (estradiol, Sigma-Aldrich), the MCF-7 cells were cultured for two days in phenol red-free DMEM (PRF-DMEM, Wako) containing 10% heat-inactivated FBS stripped of steroids by absorption to dextran-coated charcoal (DCC-FBS, Biological Industries). The cells were then cultured in a humidified 5% CO2 incubator at 37 ℃.
6
Breast Cancer Cell Line Cultivation
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Human breast cancer cell lines MCF-7, SK-BR-3 and T-47D were obtained from Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan) and American Type Culture Collection (ATCC; Manassas, VA, USA), respectively. MCF-7 cells and T-47D cells were cultured in RPMI-1640 (Wako, Osaka, Japan). SK-BR-3 cells were cultured in McCoy’s 5A (Gibco, Rockville, MD, USA). Heregulin-β1 was purchased from Wako.
7
Breast Cancer Cell Line Culture and Inhibitor Assay
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Human breast cancer cell line MCF-7 and MDA-MB-231 were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). MCF-7 and MDA-MB-231 were cultured in RPMI-1640 and DMEM medium (high glucose) supplemented with 10% heat-inactivated FBS and antibiotics (100 U/ml of penicillin and 100 μg/ml of streptomycin (Wako Pure Chemical Industries)) in a humidified 5% CO2 atmosphere at 37°C, respectively. In experiments using inhibitors, MCF-7 and MDA-MB-231 cells were treated with respective inhibitor at the indicated concentrations for 30 min prior to treatment with indicated concentrations of Helle, in the presence or absence of respective inhibitor for an additional 48 h. Helle and Areno were dissolved in dimethyl sulfoxide (DMSO), and no cytotoxicity of the final concentrations of DMSO (0.02%) was observed in the current experimental system.
8
Culturing and Treating ER-Positive Cancer Cells
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MCF-7 (breast cancer) and Ishikawa (endometrial cancer) ER-positive cancer cell lines were obtained from the European Collection of Cell Cultures (ECACC, Salisbury, UK). MCF-7 cells were maintained in Dulbecco's modified Eagle's medium (DMEM; Wako Pure Chemical Industries) supplemented with 10% fetal bovine serum (FBS; Nichirei Biosciences, Tokyo, Japan), 1% penicillin/streptomycin (Wako Pure Chemical Industries), and 2 mM L-glutamine (Life Technologies), and cultured at 37°C under a humidified 5% CO 2 atmosphere. Ishikawa cells were maintained in DMEM supplemented with 5% FBS, 1% penicillin/streptomycin, and 2 mM L-glutamine under the same atmospheric conditions as MCF-7 cells. Cells were plated onto a 24-well culture plate at 5 × 10 4 cells/well and allowed to attach overnight. The next day, the medium was replaced with phenol red-free DMEM (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% charcoal-stripped FBS (Equitech Bio, Kerrville, TX, USA), 1% penicillin/streptomycin, and 2 mM L-glutamine, and incubated for a day. Test chemicals were dissolved in DMSO and applied to cells at final DMSO concentrations of less than 0.1%. After 24 hr of chemical treatment, cells were collected and used immediately in downstream assays or stored at -80°C until use.
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