Hdl cholesterol e test

Plasma TC, HDL-C and TG were enzymatically measured by using commercial kits (Cholesterol E-test, HDL-Cholesterol E-test and Triglyceride E-test, Wako Pure Chemical Industries, Osaka, Japan). The non-HDL-C concentration was calculated as (non-HDL-C) = (TC) − (HDL-C). The hepatic and fecal lipids were extracted by the ordinary method of Folch et al. [36 (link)]. The hepatic cholesterol, TG, and fecal cholesterol concentrations were enzymatically determined by using commercial kits (Cholesterol E-test and Triglyceride E-test, Wako Pure Chemical Industries, Osaka, Japan). Fecal bile acids were extracted with hot ethanol (70 °C, 60 min) from freeze-dried feces. The extracts were enzymatically determined by using commercial kits (Total bile acid test, Wako Pure Chemical Industries, Osaka, Japan).

Serum lipids were determined enzymatically using commercial kits for total cholesterol and high density cholesterol (Total Cholesterol E-test and HDL Cholesterol E-test, respectively; Wako Pure Chemical Industries; Osaka, Japan) according to the manufacturer’s instructions.

At a determined time point for each experiment, fasting plasma samples collected in heparinized capillary tubes from the retro-orbital sinus were analyzed for plasma triglyceride (TG), total cholesterol (TC), HDL cholesterol (HDL-C), and glucose concentrations, using a Triglyceride E-Test, a Cholesterol E-Test, an HDL Cholesterol E-Test, and a Glucose CII-Test, respectively (Wako Pure Chemical Industries, Ltd., Osaka, Japan), respectively. Plasma insulin concentrations were determined with an ELISA kit (Morinaga Institute of Biological Science, Inc., Yokohama, Japan).

Serum ALT and AST levels were analyzed, as previously described^{59 ,60} . Serum concentrations of glucose, FFA, total-cholesterol, HDL-cholesterol, triglyceride, total protein, phospholipids, and ALP were measured using commercially available assay kits by FUJIFILM Wako Pure Chemical Corporation (Glucose CII-test Wako, Cholesterol E-test Wako, HDL-Cholesterol E-test Wako, NEFA C-test Wako, Triglyceride E-test Wako, Phospholipid C-test Wako, and LabAssay ALP).

Lipid metabolism levels were determined using quantitative assay kits by means of HDL cholesterol, total cholesterol, and triglyceride concentrations in the plasma with the instructions provided by the manufacturer (HDL-cholesterol E test, 431–52501; Total-cholesterol E test, 439–17501; TG E test, 432–40201, FUJI FILM Wako, Osaka, Japan). Each sample repeated at least three times.