Vivaspin centrifugal spin columns

Manufactured by Sartorius

Vivaspin centrifugal spin columns are laboratory equipment designed for rapid and efficient sample concentration and buffer exchange. These columns use centrifugal force to separate and concentrate molecules based on their size and molecular weight.

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Lab products found in correlation

Pcdna3.4 vector, by Thermo Fisher Scientific (2 mentions) Gblocks gene fragment, by Integrated DNA Technologies (2 mentions) Expi293f cells, by Thermo Fisher Scientific (2 mentions) Protein g plus agarose, by Thermo Fisher Scientific (2 mentions)

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Vivaspin (140 citations) Vivaspin columns (59 citations)

2 protocols using vivaspin centrifugal spin columns

Recombinant Antibody Production in Expi293F Cells

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Selected antibody sequences were purchased as gBlocks gene fragments (Integrated DNA Technologies) and cloned into a customized pcDNA3.4 vector (Invitrogen) containing human IgG1 or IgA1 Fc regions. VH and VL plasmids were transfected into 30 mL cultures of Expi293F cells (Invitrogen) at a 1:2 ratio and incubated at 37˚C and 8% CO₂ for 7 days. The supernatant containing secreted antibodies was collected following centrifugation (1000 g for 10 min at 4˚C), neutralized, and filtered. Antibodies were isolated using Protein G Plus agarose (IgG; Pierce Thermo Fisher Scientific) or Peptide M agarose (IgA; Invitrogen) affinity chromatography, washed with 20 column volumes of PBS, eluted with 100 mM glycine-HCl pH 2.7, and immediately neutralized with 1 M Tris-HCl pH 8.0. The antibodies were then concentrated and buffer exchanged into PBS using 10,000 MWCO Vivaspin centrifugal spin columns (Sartorius).

McDaniel J.R., Pero S.C., Voss W.N., Shukla G.S., Sun Y., Schaetzle S., Lee C.H., Horton A.P., Harlow S., Gollihar J., Ellefson J.W., Krag C.C., Tanno Y., Sidiropoulos N., Georgiou G., Ippolito G.C, & Krag D.N. (2018). High-throughput discovery of antitumor antibodies mined from sentinel lymph node B cells and circulating IgG of breast cancer patients. Cancer immunology, immunotherapy : CII, 67(5), 729-738.

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Recombinant Antibody Production in Expi293F Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Selected antibody sequences were purchased as gBlocks gene fragments (Integrated DNA Technologies) and cloned into a customized pcDNA3.4 vector (Invitrogen) containing human IgG1 Fc regions. VH and VL plasmids were transfected into 30 mL cultures of Expi293F cells (Invitrogen) at a 1:2 ratio and incubated at 37°C and 8% CO₂ for 7 days. The supernatant containing secreted antibodies was collected following centrifugation (1000 g for 10 min at 4°C), neutralized, and filtered. Antibodies were isolated using Protein G Plus agarose (Pierce Thermo Fisher Scientific) affinity chromatography, washed with 20 column volumes of PBS, eluted with 100 mM glycine-HCl pH 2.7, and neutralized with 1 M Tris-HCl pH 8.0. The antibodies were then concentrated and buffer exchanged into PBS using 10,000 MWCO Vivaspin centrifugal spin columns (Sartorius).

Lindesmith L.C., McDaniel J.R., Changela A., Verardi R., Kerr S.A., Costantini V., Brewer-Jensen P.D., Mallory M.L., Voss W.N., Boutz D.R., Blazeck J.J., Ippolito G.C., Vinje J., Kwong P.D., Georgiou G, & Baric R.S. (2019). Sera Antibody Repertoire Analyses Reveal Mechanisms of Broad and Pandemic Strain Neutralizing Responses after Human Norovirus Vaccination. Immunity, 50(6), 1530-1541.e8.

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