Amphotericin b

l-Glutamine, fetal bovine serum, penicillin, streptomycin, amphotericin B, Hanks’ salts, trypsin, DMEM, and (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were from PanEco, Moscow, Russia. 2′,7′-Dichlorodihydrofluorescein diacetate (DCFH-DA), HEPES, DMSO, d-glucose, MPP⁺, KCN, MPP⁺, l-NAME, tert-butyl hydroperoxide, and bovine serum albumin were from Sigma-Aldrich, St. Louis, MO, USA. 666-11, SB 202190, SP 600125, salirasib, FIPI, KN-93, ML-193, PSB C5, HA-1004, U-0126, KT-5720, and U-73 were from Tocris Bioscience, Bristol, UK. Total RNA Purification kit was from Jena Biosciences, Jena, Germany. MMLV reverse transcription kit and qPCR master mix qPCRmix-HS SYBR were from Evrogen, Moscow, Russia. cAMP determination kit and BrdU cell proliferation assay kit were from Abcam, Cambridge, MA, USA. DNase I was from Thermo Fisher Scientific, Waltham, MA, USA.
The peptide was synthesized by methods of classical peptide chemistry in solution using both protected and free L-amino acids, as described earlier [49 (link)]. Purity of the final compound was not less 98% (HPLC analysis, Supplementary S2).

1,2-Dioleoylphosphatidylcholine (DOPC), 1,2-dioleoylphosphatidylethanolamine (DOPE), and DOTAP chloride (USP grade) were obtained from Lipoid GmbH (Heidelberg, Germany). Sorbitan monostearate (SPAN) was obtained from Sigma-Aldrich (Merk, Buchs, Switzerland), and polysorbate 80 (PS80) and squalene (Sq) were obtained from Acros Organics, and coconut oil triacylglycerols (COTs) were obtained from commercial coconut oil.
DMEM, L-glutamine, sodium pyruvate, non-essential amino acids, penicillin, streptomycin, amphotericin B, MTT, trypsin solution in EDTA, Earle’s solution, glucose, and Versene’s solution were purchased from PanEco, Moscow, Russia. DMSO and Triton X-100 were purchased from Sigma-Aldrich, St. Louis, MO, USA.
Fetal bovine serum was purchased from PanEco, Moscow, Russia.

Biopsies of eutopic endometrium and endometriotic cysts were obtained from patients undergoing surgical treatment for ovarian endometriosis. Ethics approval for the study was obtained from the ethics committee at the National Medical Research Center for Obstetrics, Gynecology, and Perinatology, named after Academician V.I.Kulakov (protocol №9 from 16.11.2017, Ministry of Healthcare, Russian Federation). Written informed consent was obtained from each patient under study.
Tissues were collected under sterile conditions and transferred to the laboratory in a DMEM/F-12 (1:1) incubation medium without serum containing 2 mM glutamine, 100 U/mL penicillin, 100 μg/mL streptomycin, and 0.25 μg/mL amphotericin B (medium and additives were from PanEco, Russia). Cell isolation was performed not more than 30 min after tissue collection. The presence of endometriotic lesions in the portions of collected biopsies was confirmed by a pathologist after histological examination.