L glutamate oxidase ec 1.4.3.11

All chemicals were prepared and stored according to manufacturer recommendations unless otherwise noted. L-glutamate oxidase (EC 1.4.3.11) was obtained from Cosmo Bio Co. (Carlsbad, CA) and diluted in distilled, deionized water to makea1 U/µl stock solution for storage at 4°C. Sodium phosphate monobasic monohydrate, sodium phosphate dibasic anhydrous, 1,3 phenylenediamine dihydrochloride (mPD), sodium chloride, calcium chloride dehydrate, and H₂O₂ (30% in water) were obtained from Thermo Fisher Scientific (Waltham, MA). L-glutamic acid sodium salt, bovine serum albumin (BSA), glutaraldehyde, KCl, dopamine hydrochloride (DA), L-ascorbic acid (AA), were obtained from Sigma-Aldrich Co. (St. Louis, MO). LY379268 was obtained from Tocris Bioscience (Bristol, United Kingdom), while Amylo-Glo® RTD™ was obtained from Biosensis (Temecula, CA).

All chemicals were prepared and stored according to manufacturer recommendations unless otherwise noted. L-glutamate oxidase (EC 1.4.3.11) was obtained from Cosmo Bio Co. (Carlsbad, CA, USA; Cat: YMS-80049) and diluted in distilled, deionized water to make a 1U/μl stock solution for storage at 4°C. Sodium phosphate monobasic monohydrate (Cat: BP330-500), sodium phosphate dibasic anhydrous (Cat: S375-500), 1,3 phenylenediamine dihydrochloride (mPD; Cat: P017225G), sodium chloride (Cat: S271- 3), calcium chloride dehydrate (Cat: BP510-100), sucrose (Cat: 12-550-15), 30% hydrogen peroxide (H₂O₂) in water (Cat: H325-100), and riluzole (Cat: 1744-22-5) were obtained from Thermo Fisher Scientific (Waltham, MA). L-glutamic acid sodium salt (Cat: G1626), potassium chloride (KCl; Cat: P9333), bovine serum albumin (BSA; Cat: A3059), glutaraldehyde (Cat: G5882), dopamine hydrochloride (DA; Cat: H8502), and L-ascorbic acid (AA; Cat: A7056) were obtained from Sigma-Aldrich Co. (St. Louis, MO, USA). Chicken polyclonal glial fibrillary acidic protein (GFAP) primary antibody (RRID:AB_2492333) and Amylo-Glo® RTD™ (Cat: TR-300-AG) was obtained from Biosensis (Temecula, CA). Rabbit polyclonal primary antibody α7nAChR was obtained from Alomone Labs (Jerusalem, Israel; RRID:AB_10659339).