Anti vimentin 5741

WB and Immunohistochemistry (IHC) were performed as described in the previous studies (Huai et al., 2016 (link); Yang et al., 2021 (link)). Anti-Vimentin 5741 was from Cell Signaling Technology. Anti–CILP (ab192881) was from Abcam. Anti–CILP (HPA003195) was from Atlas. Anti-Flag (F1804) was from Sigma. Anti–β-actin (sc-81178) and horseradish peroxidase-conjugated secondary antibodies were from Santa Cruz Biotechnology.

Total protein was by using RIPA buffer (C1053, APPLYGEN, Beijing, China), and the protein concentration was determined by using a BCA protein assay kit (Beyotime, China). Proteins were then separated by SDS-PAGE and transferred to PVDF membranes (Millipore, Billerica, MA). After blocking for 2 h in 5% milk, membranes were washed with TBST 3 times and incubated with primary antibodies at 4 °C overnight. The membranes were then incubated with a secondary antibody (anti-mouse or anti-rabbit IgG, Cell Signaling Technology, USA) for 1 h at room temperature. After 3 washes with TBST, a Bio-Rad detection system was used to detect the bands. The antibodies used in this study were as follows: anti-GAPDH (5174), anti-TAZ (70148), anti-E-cadherin (3195), anti-Snail (3879), anti-N-cadherin (13116), anti-Vimentin (5741) and anti-Fibronectin (26836) were all obtained from Cell Signaling Technology. Anti-LATS2 (ab110780), anti-GAS1 (ab236618), anti-PFKFB3 (ab181861), anti-HK2 (ab209847) and anti-GLUT1 (ab115730) were all obtained from Abcam.

Anti-c-Myc antibody (SC-40, 1:1000), polyclonal anti-HA antibody (SC-805, 1:1000), anti-Cullin-1 antibody (SC-70895, 1:1000), and anti-Cyclin E antibody (SC-247, 1:1000) were purchased from Santa Cruz. Polyclonal anti-FLAG antibody (F2425, 1:1000), monoclonal anti-FLAG antibody (F-3165, 1:1000), anti-Vinculin antibody (V9131, 1:5000), peroxidase-conjugated anti-mouse secondary antibody (A4416, 1:3000), peroxidase-conjugated anti-rabbit secondary antibody (A4914, 1:3000) and CK1 inhibitor IC261 were purchased from Sigma. Anti-Myc-tag (2272, 1:1000), anti-GST (2625, 1:1000), anti-Vimentin (5741, 1:1000), anti-Snail (3879, 1:1000), anti-ZEB-1 (3396, 1:1000), anti-Twist1 (4119, 1:1000), anti-β-catenin (9582, 1:1000), anti-Arid1a (12354, 1:1000) and anti-BRM (11966, 1:1000) antibodies were purchased from Cell Signaling. Anti-FBW7 antibody (A301-720A, 1:1000) was purchased from Bethyl. Monoclonal anti-HA antibody (MMS-101P, 1:1000) was purchased from Covance. Anti-GFP antibody (632380, 1:1000) was purchased from Invitrogen.

The following antibodies were purchased: anti-phospho-AKT (S473) D9E #4060, anti-AKT #9272, anti-Caspase-3 #9662, anti-LC3 A/B (CST#4108), anti-vimentin #5741, anti-GM130 #12480, anti-phospho p44/42 MAPK (T202/Y204) #4370s, anti-LC3 #4108 (used for western blotting) from Cell Signaling Technology (Leiden, The Netherlands); anti-E-cadherin #610182 from BD Biosciences (San Jose, CA, USA); anti-calnexin #SPC-108 from Stress Marq Biosciences Inc. (Victoria, Canada); anti-CD107a/Lamp1 (clone H4A3) #SAB4700416, anti-αTubulin (clone B512) #T5168, anti-γtubulin #T6657 from Sigma-Aldrich (St. Louis, MI, USA); anti-GOLGIN-97 #A-21270 Thermo Fisher Scientific—Invitrogen, (Carlsbad, CA, USA); anti-LC3 (clone 5F10) #0231, used for immunofluorescence, from Nanotools (Teningen, Germany); anti-PFKM #a5477 from Abclonal Technology (Woburn, MA, USA); anti-PARP #sc-7150 from Santa Cruz Biotechnology, Inc (Santa Crus, CA, USA). Alexa-Fluor (488 and 546) secondary antibodies A11029, A11030, A11034, and A11035, were from Thermo Fisher Scientific—Invitrogen, (Carlsbad, CA, USA) and horseradish peroxidase (HRP)-conjugated secondary antibodies used for Western blot analyses were from Amersham Pharmacia (Buckinghamshire, UK).

The human lung cancer cell lines were purchased from the Cell Bank of Shanghai Institute of Cell Biology (Shanghai, China). The cell lines have been authenticated and tested by the Cell Bank. For verification, we performed mycoplasma tests in our laboratory, and the cell behavior and morphology were proved consistent with the descriptions in the Cell Bank. Anti-HOXC8 antibody (15448-1-AP), anti-E-cadherin antibody (20874-1-AP) and Anti-TGFβ1 antibody (20328-1-AP) were from ProteinTech Group (Wuhan, China). Rabbit HOXC8 antibody (HPA028911) for immunohistochemistry was purchased from Sigma (Shanghai, China). Anti-β-actin antibody (sc-1616) was obtained from Santa Cruz Biotechnology (Shanghai, China). Anti-cleaved caspase-3 (#9661), anti-cleaved PARP (#9541) and anti-vimentin (#5741) antibodies were from Cell Signaling Technology (Shanghai, China). Immunohistochemistry kit (#13079) was obtained from Cell Signaling Technology (Shanghai, China). TRIzol RNA extraction reagent was purchased from Sangon Biotech (Shanghai, China). Lipofectamine 2000 and 3000 were purchased from Life Technologies (Shanghai, China). ECL SuperSignal West Femto Maximum Sensitivity Substrate was purchased from Thermo Scientific (Shanghai, China). Cisplatin and all chemical reagents were from Sigma (Shanghai, China).

All melanoma cell lines (A375, Skmel28, and MV3), the immortalized melanocyte cell line PIG1, and the human embryonic renal cell line 293FT were obtained from the American Type Culture Collection (ATCC, USA). All cell lines were cultured as described previously^{43 (link),44 (link)}.
An anti-CSN6 antibody (NBP2-46333) was purchased from Novus Biologicals (USA). Anti-CDK9 (11705-1-AP), anti-UBR5 (66937-1-Ig), anti-HA (51064-2-AP), anti-Flag (20543-1-AP), anti-Ki67 (27309-1-AP), and anti-α-tubulin (11224-1-AP) antibodies were obtained from Proteintech (Wuhan, China). Anti-CDK1 (4539), anti-CDK4 (12790), anti-CDK2 (2546), anti-CDK6 (13331), anti-cyclin D1 (2922), anti-p21 (2947), anti-p27 (3686), anti-E-cadherin (14472), anti-N-cadherin (13116), and anti-vimentin (5741) antibodies were purchased from Cell Signaling Technology (CST, Boston, MA, USA). MG132 (M7449) and anti-BrdU (ab6326) and anti-Flag (ab213519) antibodies were obtained from Abcam (Cambridge, MA, USA). 3, 3′-Diaminobenzidine (DAB) and RIPA lysis buffer were purchased from Beyotime (Shanghai, China).