6220 tof mass spectrometer

Manufactured by Agilent Technologies

The Agilent 6220 TOF mass spectrometer is a high-performance instrument designed for accurate mass measurements. It utilizes time-of-flight technology to provide precise mass data for a wide range of analytes.

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Lab products found in correlation

1200 series hplc system, by Agilent Technologies (1 mentions) Xbridgetm c18 column, by Waters Corporation (1 mentions) Masshunter workstation, by Agilent Technologies (1 mentions) Masshunter workstation software, by Agilent Technologies (1 mentions) Agilent masshunter workstation, by Agilent Technologies (1 mentions) Agilent 1200 series hplc system, by Agilent Technologies (1 mentions)

5 protocols using 6220 tof mass spectrometer

Enzymatic Detoxification of Aflatoxins

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25 U/mL laccase from Trametes versicolor (Sigma-Aldrich CAS80498) was added to 10 μg/mL of toxin, AFB₁ or AFG₂ (both from Cayman Chemicals) separately, in acetate buffer (100 mM, pH 6.5) and left at 28 °C for 24 h. Degradation products were assayed under the following conditions: Column: Kinetex 2.6 μm EVO C18; 100 × 2.1 mm; Mobile phase A: Water 5 mM Ammonium Acetate, 0.5% Acetic Acid; Mobile phase B: Methanol 5 mM Ammonium Acetate, 0.5% Acetic Acid; Flow rate: 350 μL/min; UV Wavelength: 354, 360 nm.
The following gradient method was used in all runs. The eluent from the column was directed into the electrospray source of an Agilent 6220 TOF mass spectrometer operated in positive ionization mode. Data was converted into the mzML file format and analyzed using the MZMine software. Supplementary Figs. S2 and S3 show the resulting traces for AFB₁ and AFG₂, respectively. AFB₁ and AFG₂ detoxification byproducts are shown in the supplements.

Zaccaria M., Dawson W., Russel Kish D., Reverberi M., Bonaccorsi di Patti M.C., Domin M., Cristiglio V., Chan B., Dellafiora L., Gabel F., Nakajima T., Genovese L, & Momeni B. (2023). Experimental–theoretical study of laccase as a detoxifier of aflatoxins. Scientific Reports, 13, 860.

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Comprehensive 2D NK-92MI/CMC/TOFMS Analysis

Cited 2 times

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Fig. 2 shows that the NK-92MI column/C₁₈ column/TOFMS system was mainly composed of an Agilent 1,200 series HPLC system and an Agilent 6,220 TOF mass spectrometer. The equipment was controlled using an Agilent MassHunter workstation. The first-dimensional column was an NK-92MI/CMC column. The mobile phase was ammonia acetate (10 mM) and the flow was 0.2 mL/min. The second-dimensional column was an XBridge TM C₁₈ column (100 mm × 3.0 mm i.d., 3.5 μm; Waters, Wexford, Ireland). Using a linear elution procedure, a mixture of 0.1% (V/V) formic acid and acetonitrile was used as the mobile phase [15 (link)]. The flow velocity was set to 0.8 mL/min. More detailed information about the comprehensive 2D system is available in previous reports [18 (link),19 (link)].Fig. 2

Brief scheme of the comprehensive two-dimensional natural killer (NK)-92MI/cell membrane chromatography (CMC)/CMC column/C₁₈ column/time-of-flight mass spectrometry (TOFMS) system: (A) position 1 and (B) position 2.

Fig. 2

Chai X., Gu Y., Lv L., Chen C., Feng F., Cao Y., Liu Y., Zhu Z., Hong Z., Chai Y, & Chen X. (2022). Screening of immune cell activators from Astragali Radix using a comprehensive two-dimensional NK-92MI cell membrane chromatography/C18 column/time-of-flight mass spectrometry system. Journal of Pharmaceutical Analysis, 12(5), 725-732.

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Lipid Profiling of M. tuberculosis H37Rv and Mutant

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Total lipids from M. tuberculosis H37Rv and the Δmce1 mutant grown on minimal Sauton medium supplemented with palmitic acid as the carbon source were analysed by LC/MS as described by Sartain et al. [17 (link)]. A high resolution Agilent 6220 TOF mass spectrometer interfaced to a LC was used. Data files were analyzed with Agilent’s Mass hunter workstation software (Version B.02.00, build 2.0.197.0) to identify compounds using ‘molecular feature extractor’. The Agilent mass profiler program was used to compare lipids and mycolic acids present in the sample. Most compounds were identified using the lipid database developed by [17 (link)]. Compounds of interest were semi-quantified by comparing their relative abundance in the samples.

Forrellad M.A., McNeil M., Santangelo M.P., Blanco F.C., García E., Klepp L.I., Huff J., Niederweis M., Jackson M, & Bigi F. (2014). Role of the Mce1 transporter in the lipid homeostasis of Mycobacterium tuberculosis. Tuberculosis (Edinburgh, Scotland), 94(2), 170-177.

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Analytical Profiling of Aflatoxin Degradation

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For the discovery of AF degradation products, four solutions containing different aflatoxins (AF B₁, B₂, G₁ or G₂; 0.2 mg each) and APAB (4 mg) have been dissolved in 4 mL of water and kept at 40 °C for 30 min. Then, AFs have been extracted from 1 mL aliquots of the solution (as reported below) for analysis. LC/MS analysis was performed with the following equipment and reagents: Kinetex column 2.6 µM EVO C18, 100 × 2.1 mm; mobile phase A: Water 5 mM Ammonium Acetate, 0.5% Acetic Acid; mobile phase B: Methanol, 5 mM Ammonium Acetate, 0.5% Acetic Acid, at a flow rate of 350 µl/min, and with UV wavelengths at 354 and 360 nm (Table 1).Table 1

Gradient scheme.

Time (min)	%A	%B
Initial	90	10
3	90	10
10	30	70
10.1	10	90
12	10	90
12.1	90	10
15	90	10

The eluent from the column was directed into the electrospray source of an Agilent 6220 TOF mass spectrometer operated in positive ionization mode. Data was converted into mzML file format and analyzed using MZMine software.

Finotti E., Parroni A., Zaccaria M., Domin M., Momeni B., Fanelli C, & Reverberi M. (2021). Aflatoxins are natural scavengers of reactive oxygen species. Scientific Reports, 11, 16024.

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Screening Bioactive Compounds from Scutellariae Radix

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Briefly, a TKT biological chromatographic column packed with mercaptopropyltrimethoxysilane‐modified TKT‐immobilised silica stationary phase was prepared. Binding ligands to TKT from Scutellariae Radix extracts were screened by the online comprehensive two‐dimensional TKT biological chromatography/high performance liquid chromatography/time‐of‐flight mass spectrometry system equipped with an Agilent 1200 series HPLC system, a 6220 TOF mass spectrometer, and an Agilent MassHunter Workstation (Agilent Technologies). Detailed experimental information including column packing, screening, validation, data processing, and signal deduction were performed according to our previous studies.¹⁷, ¹⁸

Jia D., Liu C., Zhu Z., Cao Y., Wen W., Hong Z., Liu Y., Liu E., Chen L., Chen C., Gu Y., Jiao B., Chai Y., Wang H., Fu J, & Chen X. (2022). Novel transketolase inhibitor oroxylin A suppresses the non‐oxidative pentose phosphate pathway and hepatocellular carcinoma tumour growth in mice and patient‐derived organoids. Clinical and Translational Medicine, 12(11), e1095.

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