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Control non targeting shrna vector

Manufactured by Merck Group
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Sourced in United States

The Control non-targeting shRNA vector is a laboratory tool used for gene knockdown studies. It contains a non-targeting short hairpin RNA (shRNA) sequence that does not target any known gene. This vector can be used as a control to assess the effects of shRNA-mediated gene silencing in experimental settings.

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Lab products found in correlation

Mission trc ick shrna target set, by Merck Group (2 mentions) Pcdna3, by Addgene (1 mentions) Human insulin, by Eli Lilly (1 mentions)

Close spelling variants of this product

Non-targeting shRNA control Non-targeting shRNA ShRNA targeting Control shRNA ShRNA vectors Control vector

2 protocols using control non targeting shrna vector

1

Silencing ICK in HCT-8 Cells

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The MISSION TRC ICK shRNA Target Set and the control non-targeting shRNA vector were obtained from Sigma, as described in [10] (link). Lentivector particles were generated in HEK293T cells as described in [10] (link), [11] (link). Exponentially growing HCT-8 cells at about 50–60% confluence were infected with lentiviruses expressing either the ICK shRNA or the control shRNA for overnight (12–14 hr) before change of medium. Twenty four hours after infection, cells were plated at about 6–8×105 cells/ml in 10 cm dishes and grown for three to four days in the presence of 5 µg/ml puromycin. Infected cells were harvested for either determination of viable cell number and apoptosis or for protein extraction.
Bolick D.T., Chen T., O. Alves L.A., Tong Y., Wu D., Joyner LT I.I., Oriá R.B., Guerrant R.L, & Fu Z. (2014). Intestinal Cell Kinase Is a Novel Participant in Intestinal Cell Signaling Responses to Protein Malnutrition. PLoS ONE, 9(9), e106902.
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2

Characterization of ICK and GSK3β Kinases

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Human insulin was from Lilly USA (Indianapolis, IN, USA). Calyculin A was from Calbiochem (distributed through MilliporeSigma, Burlington, MA, USA). All synthetic polypeptides were from ABI Scientific (Sterling, VA, USA). The MISSION TRC ICK shRNA Target Set and the control non-targeting shRNA vector were obtained from Sigma (St. Louis, MO, USA), as described in [28 (link)]. GST-ICK WT (wild type) and K33M (kinase dead) in pEBG-GST were described previously in [29 (link), 30 (link)]. HA-GSK3β WT, S9A, and K85A in pCDNA3 (Addgene plasmids #14753, #14754, and #14755) were gifts from Dr. Jim Woodgett [14 (link)] through Addgene (Cambridge, MA, USA). HA-GSK3β T7A and T8A mutants were generated using the QuikChange Site-Directed Mutagenesis Kit from Stratagene (San Diego, CA, USA) and confirmed by DNA sequencing at DNA Sciences Core, University of Virginia.
Tong Y., Park S., Wu D., Harris T.E., Moskaluk C.A., Brautigan D.L, & Fu Z. (2018). Modulation of GSK3β autoinhibition by Thr-7 and Thr-8. FEBS letters, 592(4), 537-546.
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