Sw1116

The DLD1 (ATCC CCL-221), HCT116 (ATCC CCL-247), LoVo (ATCC CCL-229), SW620 (ATCC CCL-227), SW1116 (ATCC CCL-233) and 293T (ATCC CRL-11268) cell lines were purchased from American Type Culture Collection (ATCC). The TC71 patient-derived xenograft cell line was obtained from XENTECH (MRF reference: XTM-233_CXT-399/R5700).
DLD1, HCT116, LoVo, SW620 and SW1116 were cultured in RPMI-1640 medium (Invitrogen, Carlsbad, USA) with 10% fetal bovine serum (HyClone, USA) as routine. 293T was maintained in Dulbecco’s modified Eagle’s medium (Invitrogen, Carlsbad, USA) supplemented with 10% fetal bovine serum. TC71 was propagated in Advanced DMEM/F12 supplemented with 8% FBS, 1% antibiotics and 1% Glutamin. All cells were grown in a humidified incubator at 37 °C with 5% CO₂. All the cell lines were authenticated 3 months before the beginning of the study based on viability and morphology by the suppliers. Cells have not been in culture for longer than 2 months.

The immortalized human colorectal epithelial cell line (FHC) and CRC cell lines HCT116, DLD-1, SW480, LOVO, HT29, and RKO were purchased from American Type Culture Collection (ATCC). SW1116 and SW620 cells were obtained from the Cell Resource Center, Shanghai Institute of Biochemistry and Cell Biology at the Chinese Academy of Sciences. All cells were cultivated in a humidified environment with 5% CO₂ at 37 °C in recommended growth medium added with 10% fetal bovine serum. FHC cells: Dulbecco’s modified Eagle’s medium/F12 Medium (Gibco, USA); SW1116, SW620 and DLD-1 cells: RPMI-1640 Medium (Gibco, USA); HCT116 and HT29 cells: McCoy’s 5a Medium (Gibco, USA); SW480 cells: L-15 medium (Gibco, USA); LoVo cells: F-12K Medium (Gibco, USA).

Twenty pairs of fresh frozen CRC tissues (tumor and corresponding adjacent) were obtained from the Sun Yat-sen University Cancer Center (SYSUCC), Guangzhou, China, between December 2015 and June 2017. All samples were selected and confirmed histologically based on the following criteria: primary resection of the tumor with detailed follow-up data and no preoperative anticancer treatment. The human CRC cell lines (HCT116, SW480, HT29, LOVO, SW1116, SW640) and controlled cell line (HEK-293T) were obtained from the Chinese Academy of Sciences, Shanghai Institutes for Cell Resource Center. HCT116 and HT29 were maintained in McCoy's 5A with L-glutamine (HyClone, Logan, UT, USA). SW480 was cultured in incomplete Leibovitz's L-15 medium (KeyGEN, Nanjing, Jiangsu, China) media supplemented with 10% heat-inactivated fetal bovine serum (Gibco, Carlsbad, CA, USA). The LOVO, SW1116, SW640, and HEK-293T were supported with DMEM (Gibco, Carlsbad, CA, USA) with 10% heat-inactivated fetal bovine serum (Gibco, Carlsbad, CA, USA). Cells were grown in a humidified incubator at 37 °C with 5% CO₂. This present study was approved by the Ethics Committee of Sun Yat-sen University Cancer Center (NO. L102012020000B). All participants were recruited after providing signed informed consent.

Human colorectal cancer cell lines including LoVo, Caco-2, HT-29, HCT 116, SW480, SW620, SW1116 and Human kidney 293T cell were obtained from ATCC (Manassas, VA, USA). LoVo was maintained with F12K medium (SIGMA, USA); HT-29 and HCT 116 were maintained with McCoy's 5A (SIGMA, USA); SW480, SW620, and SW1116 were cultured in Leibovitz' L-15 (GIBCO, USA) with 10% FBS (GIBCO, USA); Caco-2 was cultured in MEM (GIBCO, USA) with 20% FBS (GIBCO, USA); 293T was maintained in DMEM (GIBCO, USA) with 10% FBS (GIBCO, USA). The SW480, SW620, and SW1116 were incubated at 37°C without CO₂; the other cells were cultured in 37°C with 5% CO₂.

The human CRC cell lines HCT 116, LoVo, SW1116, and SW620 were acquired from Shanghai Cell bank of Chinese Academy of Sciences (Shanghai, China), apart from the human colon epithelial cell line FHC which were from Kunming Cell bank of Chinese Academy of Sciences (Kunming, China). The cells were cultured in the respective recommended media and culture conditions: McCOY's 5A (SIGMA, USA) + 10% fetal bovine serum (FBS, GIBCO, USA) and 5% CO₂ for HCT 116; Ham's F-12K Medium (Invitrogen, USA) + 10% FBS and 5% CO₂ for LoVo; Leibovitz's L-15 Medium (GIBCO, USA) + 10% FBS (GIBCO, USA) and 100% air for SW1116, and SW620; RPMI-1640 (GIBCO, USA) + 10% FBS (GIBCO, USA) for FHC. The culture temperature was 37 ℃.