To analyze IL–33 and TSLP expression in the lungs section, the following antibodies were used for immunostaining: mouse IL–33 (0.2 mg/ml, AF3626, R&D Systems, Minneapolis, MN); mouse TSLP biotinylated (0.2 mg/ml, BAF555, R&D Systems) and respective isotype controls (R&D Systems). IL–33 and TSLP antibody–antigen complex were detected using Cy3 donkey anti–goat IgG (1:10000) (Invitrogen/ Molecular probes, Grand Island, NY). Slides were counterstained with DAPI (Vector Labs, Burlingame, CA). Images were obtained using a Nikon A1R si microscope.
Cy3 donkey anti goat igg
Manufactured by Thermo Fisher Scientific
Sourced in United States
Cy3 donkey anti–goat IgG is a secondary antibody conjugated with the Cyanine 3 (Cy3) fluorescent dye. It is designed to detect and bind to goat primary antibodies in various immunoassay applications.
Automatically generated - may contain errors
Lab products found in correlation
Af3626, by R&D Systems (1 mentions)
A1rsi microscope, by Nikon (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Vector Laboratories (1 mentions)
Alexa fluor 488 anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 488 anti mouse igg, by Thermo Fisher Scientific (1 mentions)
Anti proliferating cell nuclear antigen antibody, by Merck Group (1 mentions)
Anti sox9 antibody, by Merck Group (1 mentions)
2 protocols using cy3 donkey anti goat igg
1
Lung IL-33 and TSLP Expression
2
Immunohistochemical Evaluation of Gastric Cells
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Gastric specimens were sectioned at a thickness of 3 μm and used for immunohistochemistry as described previously.25 (link),26 (link) Primary antisera were diluted in phosphate-buffered saline (PBS) and incubated overnight at 4°C. The next day, slides were washed in PBS and incubated with secondary antibody at 37°C for 30 minutes. Cy3 Donkey Anti-Goat IgG, Cy3 Donkey Anti-Rabbit IgG, Alexa Fluor 488 Anti-Rabbit IgG, and Alexa Fluor 488 Anti-Mouse IgG (Molecular Probes Inc., Eugene, OR, USA) were used as the secondary antibodies.
The panel of primary antisera included anti-COX-2 antibody (sc-1747: dilution of 1:200, goat polyclonal; Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-DCAMKL1 antibody (dilution of 1:30, rabbit polyclonal; Abgent, San Diego, CA, USA), anti-proliferating cell nuclear antigen (PCNA) antibody (dilution of 1:2,000, mouse monoclonal; Sigma-Aldrich, St. Louis, MO, USA), and anti-Sox9 antibody (dilution of 1:1,000, rabbit polyclonal; Millipore, Temecula, CA, USA).
The number of positive stained cells for COX-2 or DCAMKL1 was counted in five microscopic fields and then the mean value was calculated.
The panel of primary antisera included anti-COX-2 antibody (sc-1747: dilution of 1:200, goat polyclonal; Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-DCAMKL1 antibody (dilution of 1:30, rabbit polyclonal; Abgent, San Diego, CA, USA), anti-proliferating cell nuclear antigen (PCNA) antibody (dilution of 1:2,000, mouse monoclonal; Sigma-Aldrich, St. Louis, MO, USA), and anti-Sox9 antibody (dilution of 1:1,000, rabbit polyclonal; Millipore, Temecula, CA, USA).
The number of positive stained cells for COX-2 or DCAMKL1 was counted in five microscopic fields and then the mean value was calculated.
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