Emr 500

This cross-sectional study was performed on psychiatric patients referred to Psychiatry outpatient clinic of Healthcare Application and Research Hospital. All patients who were referred to the psychiatry center and would like to contribute in our study were questioned about demographic characteristics, abortion, the habit of feeding cats and consumption of raw or undercooked meat. All patients and healthy individuals who gave written consent were recruited in this study using the convenience sampling method.
The study included the samples from 175 psychiatric patients (65 patients with schizophrenia, 46 Depression and 64 Bipolar Affective Disorder (BAD)) aged from 18-80 years old and samples from 100 the same age range healthy individuals as a control group. A blood sample of 2-3 ml was taken from patients aged. The blood sample was centrifuged at 1500 rpm for 10 mins for separation of the serum. The serum samples were stored at -20°C until assay. The Toxo-IgG and Toxo-IgM antibodies were investigated in the test serums with the enzyme-linked immunosor-bent assay (ELISA) using Dia Pro (Milan, Italy) commercial kits with 100% sensitivity and 100% specificity. The ELISA was performed and evaluated according to the Dia Pro (Milan, Italy) kits procedure. Absorbance plate wells were read at a wavelength of 450 nm with a plate reader (Labomed EMR-500, USA).

The serums stored at -20 °C and the kits stored at 2°-8 °C were taken to room temperature. The patient samples were diluted at the rate of 1:101 with 1000 μl sample diluent+10 μl sample and 100 μl was pipetted into wells from the controls and calibrators and 100 μl was added from the diluent. The samples were kept at 37 °C for 60 min then washed 5 times with 60 ml Washbuf 1200 ml distilled water. Diluent of 1.9 μl was added to Toxo IgM Ag and antigen antibody complex was formed by adding 100 μl from the conjugate to each of the Toxo Ag. From the prepared antigen-antibody complexes, 100 μl was added to each of the wells and they were kept at 37 °C for 60 min, then washed 5 times with 60 ml Washbuf 1200 ml distilled water. 100 μl chromogen substrate was added to all the wells and incubated in the dark at room temperature for 20 min. Absorbance plate wells were read at a wavelength of 450 nm with a plate reader (Labomed EMR-500, USA).

The serums stored at -20 °C and the kits stored at 2°-8 °C were taken to room temperature. The patient samples were diluted at the rate of 1:101 with 1000 μl sample diluent+10 μl sample and 100 μl was pipetted into wells from positive and negative controls and calibrators (1-6). The wells were marked and covered, then incubated at 37 °C for 60 min. They were then washed 5 times in the washing device and 100 μl enzyme conjugate was added to the wells and incubated again at 37 °C for 60 min. They were then washed 5 times in the washing device and 100 μl chromogen substrate was added to the wells and incubated in the dark at room temperature (18°-24 °C) for 20 min. Stop solution of 100 μl was added to all the wells and the samples were then examined with the ELISA reader (Labomed EMR-500, USA) at 450 nm wavelength.