Snu 475

Human liver cell line (THLE-3), cancer cell lines (SNU-475 and SNU-423), normal B lymphocyte (CCL-156) and normal monocyte/macrophage (CRL-9855) were purchased from American Type Culture Collection (ATCC, Manassas, Virginia, USA). SNU-475, SNU-423 and CCL-156 were cultured in Roswell Park Memorial Institute medium (RPMI-1640) supplemented with 10% heat inactivated fetal bovine serum (FBS, Sigma-Aldrich, St. Louis, MO) and 1% penicillin and streptomycin. THLE-3 was grown in Bronchial Epithelial Cell Growth Medium (BGEM) bulletkitTM (Lonza/Clonetics Corporation, Walksrsville, MD 21793) whereas, CRL-9855 was grown in Iscove’s Modified Dulbecco’s Medium (IMDM, ATCC 30-2005, Manassas, Virginia, USA) with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; fetal bovine serum, 10%. All cell lines were cultured in a humidified incubator with 5% CO₂ at 37 °C. All experiments were conducted on cell lines with passage number 1 to 10.

Human liver cell line (THLE-3) and cancer cell lines (SNU-475 and SNU-423) were purchased from American Type Culture Collection (ATCC, Manassas, Virginia, USA). SNU-475 and 423 were cultured in Roswell Park Memorial Institute medium (RPMI-1640) supplemented with 10% heat inactivated fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MO) and 1% penicillin and streptomycin. THLE-3 was grown in Bronchial Epithelial Cell Growth Medium (BGEM) bulletkitTM (Lonza/Clonetics Corporation, Walksrsville, MD 21793). All cell lines were cultured in a humidified incubator with 5% CO₂ at 37 °C. All experiments were conducted on cell lines with passage number 1–10.

The human HCC cell lines (HepG2, SNU449, and SNU475; American Type Culture Collection [ATCC], Manassas, VA, USA) and the normal hepatocyte cell line MIHA (ATCC) were cultured in DMEM (for MIHA and HepG2 cell lines; Sigma-Aldrich, St. Louis, MO, USA) or RPMI 1640 (for SNU449 and SNU475 cell lines; Sigma-Aldrich), supplemented with 10% heat-inactivated fetal bovine serum (FBS; GE Healthcare HyClone, Pittsburgh, PA, USA), 100 U/mL penicillin (GE Healthcare HyClone), and 100 mg/mL streptomycin (GE Healthcare HyClone). Cells were cultured at 37°C in a humidified atmosphere of 5% CO₂. All cell lines were tested for possible mycoplasma contamination using the BioMycoX Mycoplasma PCR Detection Kit (CS-D-25; Cellsafe, Suwon, Republic of Korea) and were authenticated using short tandem repeat (STR) profiling.