Ab5454

Manufactured by Abcam

Ab5454 is a laboratory reagent designed for use in research applications. It is a high-quality product intended to meet the needs of scientists and researchers in their work.

Automatically generated - may contain errors

Lab products found in correlation

Ab39670, by Abcam (2 mentions) Icos c398.4a, by BioLegend (2 mentions) Foxp3 fjk 16s, by Thermo Fisher Scientific (2 mentions) Cd25 7d4, by BD (2 mentions) Il 17a tc11 18h10, by BD (2 mentions) Ifn g xmg1, by BD (2 mentions) Lag3 46 2231 80, by Thermo Fisher Scientific (2 mentions) Nrp1 fab566a, by R&D Systems (2 mentions) Ctla4 14d3, by Thermo Fisher Scientific (2 mentions) Gitr dta 1, by Thermo Fisher Scientific (2 mentions) P27 sc 776, by Santa Cruz Biotechnology (2 mentions) Ab85047, by Abcam (2 mentions) P foxo1 9461s, by Cell Signaling Technology (2 mentions) Brdu b44, by BD (2 mentions) Foxo1, by Abcam (2 mentions) Cd62l mel 14, by BioLegend (2 mentions) Cd44 im7, by BioLegend (2 mentions) Raptor, by Abcam (2 mentions) Ki67 sola15, by Thermo Fisher Scientific (2 mentions) Cd4 h129, by BD (2 mentions)

4 protocols using ab5454

Multiparameter FACS and Cell Sorting

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The following antibodies were used in fluorescence-activated cell sorting (FACS) and cell sorting: CD45.1 (A20, BioLegend), CD45.2 (104, BioLegend), CD4 (H129.19, BD Pharmingen), CD25 (7D4, BD Pharmingen), IL-17A (TC11-18H10.1, BD Pharmingen), IFN-g (XMG1.2, BD Pharmingen), CD44 (IM7, Bio-Legend), CD62L (MEL-14, BioLegend), CD25 (7D4, BD Pharmingen), Lag3 (46-2231-80, eBioscience), Nrp1 (FAB566A, R&D Systems), Ki67 (SolA15, eBioscience), CTLA4 (14D3, eBioscience), GITR (DTA-1, eBioscience), ICOS (C398.4A, BioLegend), Foxp3 (FJK-16 s, eBioscience), p27 (sc-776, Santa Cruz Biotechnology), p-p27 (ab85047, Abcam), Foxo1 (ab39670, Abcam), p-Foxo1 (9461S, Cell Signaling Technology), Raptor (ab5454, Abcam), Brdu (B44, BD Pharmingen).

Wu C., Chen Z., Xiao S., Thalhamer T., Madi A., Han T, & Kuchroo V. (2018). SGK1 Governs the Reciprocal Development of Th17 and Regulatory T Cells. Cell reports, 22(3), 653-665.

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Immunohistochemical Analysis of Spinal Cord

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Spinal cord of P90 mice was isolated and immediately fixed by using 4% PFA followed by 20% sucrose incubation. Then, the samples were embedded by freezing in TissueTek^® OCT. Next, 10 µm lumbar spinal cord sections were prepared using Cryotome™ FSE cryostat (Thermo Fisher Scientific). Sections were rinsed in PBS and then permeabilized with 0.1% Triton X‐100, 5% Goat Serum (GS), 1 mg/ml bovine serum albumin IgG, and protease free (BSA) in PBS. Primary antibodies against NeuN 1:500 (Milipore MAB377)/CRMP4 1:400 (Millipore AB5454)/GFP 1:400 (Abcam ab13970)/activated Caspase 3 1:15 (Biovision 3015‐100) were diluted in blocking solution, 5% GS, 1 mg/ml BSA in PBS, and incubated overnight at 4°C. Samples were incubated with species‐specific fluorescent secondary antibodies for 2 h at room temperature. ProLong antifade medium with Dapi (Molecular Probes) was added, and the samples were covered with a #1.5, 18 × 18 mm cover slide.

Maimon R., Ankol L., Gradus Pery T., Altman T., Ionescu A., Weissova R., Ostrovsky M., Tank E., Alexandra G., Shelestovich N., Opatowsky Y., Dori A., Barmada S., Balastik M, & Perlson E. (2021). A CRMP4‐dependent retrograde axon‐to‐soma death signal in amyotrophic lateral sclerosis. The EMBO Journal, 40(17), e107586.

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Multiparameter FACS and Cell Sorting

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Wu C., Chen Z., Xiao S., Thalhamer T., Madi A., Han T, & Kuchroo V. (2018). SGK1 Governs the Reciprocal Development of Th17 and Regulatory T Cells. Cell reports, 22(3), 653-665.

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Immunohistochemical Analysis of Sciatic Nerves

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Sciatic nerves of P90 mice were isolated and immediately fixed by using 4% PFA followed by 20% sucrose incubation. Then, the samples were embedded by freezing in Tissue‐Tek^® OCT. Next, 10 µm lumbar sciatic nerve sections were prepared using Cryotome™ FSE cryostat (Thermo Fisher Scientific). Sections were rinsed in PBS and then permeabilized with 0.1% Triton X‐100, 5% Goat Serum (GS), 1 mg/ml bovine serum albumin IgG, and protease free (BSA) in PBS. Primary antibodies against NFH 1:500(Abcam ab72996/ Covance smi31p/ Covance smi32p)/CRMP4 1:400 (Millipore AB5454)/GFP 1:400 (Abcam ab13970) were diluted in blocking solution, 5% GS, 1 mg/ml BSA in PBS, and incubated overnight at 4°C. Samples were incubated with species‐specific fluorescent secondary antibodies for 2 h at room temperature. ProLong antifade medium (Molecular Probes) was added, and the samples were covered with a #1.5, 18 × 18 mm cover slide.

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