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Anti flip antibody

Manufactured by Enzo Life Sciences
Sourced in United States

The Anti-FLIP antibody is a research tool used to detect and quantify the expression of the cellular FLICE-inhibitory protein (c-FLIP) in biological samples. c-FLIP is an important regulator of apoptosis, or programmed cell death. This antibody can be utilized in various immunoassay techniques, such as Western blotting and immunohistochemistry, to study the role of c-FLIP in cellular processes and disease states.

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2 protocols using anti flip antibody

1

Autophagy and mTOR Pathway Regulation

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Antibodies against phospho-GS(Ser641), LC3, p62, AMPK, phospho-AMPK (Thr172), mTOR, phospho-mTOR(Ser2448), phospho-p70S6K (Thr389), p70S6K, phospho-4E-BP1(Thr37/46), 4E-BP1 were purchased from Cell Signaling (Danvers, MA, USA). Anti-GSK-3β and β-catenin antibodies were purchased from Santa Cruz (Santa Cruz, CA, USA). Antibodies against LAMP1, cathepsin B, TFEB, H3, and MG132, BAF were purchased from Sigma (St. Louis, MO, USA). Anti-FLIP antibody was purchased from ENZO Life sciences (Farmingdale, NY, USA). Human recombinant TRAIL was purchased from PeproTech (Rocky Hill, NJ, USA).GSK-3β inhibitors AR-A014418, SB216763, and SB415286 were obtained from Selleck (Shanghai, China).
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2

Apoptosis Pathway Antibodies and Reagents

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RPMI1640, tunicamycin, PI, anti-α-tubulin antibody (B-5-1-2), anti-β-actin antibody (AC-74) and anti-FLAG antibody (M2) were purchased from Sigma. Z-VAD-fmk and MG132 were from the Peptide Institute. Etoposide was obtained from Bristol-Myers Squibb. Bortezomib was from LC Laboratories. The anti-Hsp90 antibody (68), anti-Mcl-1 antibody (22), anti-Bcl-x antibody and anti-Bcl-2 antibody (7) were from BD Biosciences. The anti-PARP antibody (46D11) and anti-β-catenin antibody were from Cell Signaling Technology. The anti-caspase 3 antibody (H-277), anti-CHOP antibody (R-20), anti-GAPDH antibody (FL-335) and anti-caspase 9 antibody (H-17) were from Santa Cruz. The anti-XIAP antibody, anti-caspase 8 antibody (5F7), anti-caspase 9 antibody (5B4) and anti-caspase 10 antibody (4C1) were from Medical & Biological Laboratories. The anti-c-IAP1 antibody was from R&D Systems. The anti-FLIP antibody and anti-Apaf1 antibody were from Enzo. The anti-Apollon antibody [20] (link), recombinant Stx1 and Stx2 [21] (link) and Stx1 A-subunit mutant (E167Q/R170L) [22] (link) were prepared as described previously.
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