Log In Sign up
The largest database of trusted experimental protocols

Premade adenovirus type 5 particles

Manufactured by Vector Biolabs
Visit Supplier

Premade adenovirus type 5 particles. Purified and concentrated adenovirus type 5 particles for use in research applications.

Automatically generated - may contain errors

Lab products found in correlation

Px330 u6 chimeric bb cbh hspcas9, by Addgene (2 mentions) Pguide, by Addgene (2 mentions) Pcas9 gfp, by Addgene (2 mentions)

2 protocols using premade adenovirus type 5 particles

1

Adenoviral Constructs for CRISPR Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols
The BE3-encoding gene and synthetic polyadenylation sequence from pCMV-BE3, the CAG reporter from pCas9_GFP (Addgene plasmid #44719), and the U6 promoter-driven gRNA cassette from pGuide (Addgene plasmid #64711) with the protospacer sequence 5’-CAGGTTCCATGGGATGCTCT-3’ (for Pcsk9 studies), the protospacer sequence 5’-CATTCAACGTCACAACCACC-3’ (for the Hpd studies), or the protospacer sequence 5’-GGTGCTAGCCTTGCGTTCCG-3’ (control studies: irrelevant protospacer not matching any sequence in the mouse genome) were cloned into pDUAL-Basic expression vector. For R26mTmG/+ experiments, which used SpCas9 and not the BE3, the mTmG protospacer (5’-ATTATACGAAGTTATATTAA-3’) was cloned into plasmid pX330-U6-Chimeric_BB-CBh-hSpCas9 (a gift from Feng Zhang; Addgene plasmid # 42230). Vector Biolabs (Malvern, PA) used these constructs to generate recombinant adenovirus type 5 particles. Premade adenovirus type 5 particles containing the GFP transgene or Cre recombinase under a CMV promoter were obtained from Vector Biolabs. Ad viral vectors are referred to as Ad.BE3.Pcsk9, Ad.BE3.Hpd, Ad.BE3.Null, Ad.SpCas9.mTmG, Ad.GFP, and Ad.Cre, and the titers are indicated in Supplementary Table 2.
Rossidis A.C., Stratigis J.D., Chadwick A.C., Hartman H.A., Ahn N.J., Li H., Singh K., Coons B.E., Li L., Lv W., Zoltick P.W., Alapati D., Zacharias W., Jain R., Morrisey E.E., Musunuru K, & Peranteau W.H. (2018). In utero CRISPR-mediated therapeutic editing of metabolic genes. Nature medicine, 24(10), 1513-1518.
+ Open protocol
+ Expand
2

Adenoviral Constructs for CRISPR Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols
The BE3-encoding gene and synthetic polyadenylation sequence from pCMV-BE3, the CAG reporter from pCas9_GFP (Addgene plasmid #44719), and the U6 promoter-driven gRNA cassette from pGuide (Addgene plasmid #64711) with the protospacer sequence 5’-CAGGTTCCATGGGATGCTCT-3’ (for Pcsk9 studies), the protospacer sequence 5’-CATTCAACGTCACAACCACC-3’ (for the Hpd studies), or the protospacer sequence 5’-GGTGCTAGCCTTGCGTTCCG-3’ (control studies: irrelevant protospacer not matching any sequence in the mouse genome) were cloned into pDUAL-Basic expression vector. For R26mTmG/+ experiments, which used SpCas9 and not the BE3, the mTmG protospacer (5’-ATTATACGAAGTTATATTAA-3’) was cloned into plasmid pX330-U6-Chimeric_BB-CBh-hSpCas9 (a gift from Feng Zhang; Addgene plasmid # 42230). Vector Biolabs (Malvern, PA) used these constructs to generate recombinant adenovirus type 5 particles. Premade adenovirus type 5 particles containing the GFP transgene or Cre recombinase under a CMV promoter were obtained from Vector Biolabs. Ad viral vectors are referred to as Ad.BE3.Pcsk9, Ad.BE3.Hpd, Ad.BE3.Null, Ad.SpCas9.mTmG, Ad.GFP, and Ad.Cre, and the titers are indicated in Supplementary Table 2.
Rossidis A.C., Stratigis J.D., Chadwick A.C., Hartman H.A., Ahn N.J., Li H., Singh K., Coons B.E., Li L., Lv W., Zoltick P.W., Alapati D., Zacharias W., Jain R., Morrisey E.E., Musunuru K, & Peranteau W.H. (2018). In utero CRISPR-mediated therapeutic editing of metabolic genes. Nature medicine, 24(10), 1513-1518.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!