The capillary hydrophilic interaction chromatography (cHILIC) was performed on a Shimadzu Prominence nano-flow liquid chromatography system (Shimadzu, Tokyo, Japan) with two LC-20AD nano pumps, two vacuum degassers, a LC-20AB high performance liquid chromatography (HPLC) pump, a SIL-20AC HT auto-sampler, and a nano-flow control valve. The electrospray ionization/tandem mass spectrometry (ESI-MS/MS) experiment for detecting the genomic 5-mdC contents was detailly described in the previous study [29 (link)]. The results showed linearity within the range of 0.05% - 10% (molar ratio of 5-mdC/dC) with a coefficient value (R2) 0.996.
Lc 20ad nano pump
Manufactured by Shimadzu
Sourced in Japan
The LC-20AD nano pumps are high-performance liquid chromatography pumps designed for nano-flow applications. They deliver precise and stable flow rates ranging from 1 nL/min to 10 μL/min, making them suitable for various analytical techniques that require low flow rates, such as nano-LC, capillary LC, and micro-LC.
Automatically generated - may contain errors
Lab products found in correlation
Sil 20ac ht autosampler, by Shimadzu (5 mentions)
Lc 20ab hplc pump, by Shimadzu (4 mentions)
Prominence nano ow liquid chromatography system, by Shimadzu (3 mentions)
Ultra plus field emission scanning electron microscope, by Zeiss (2 mentions)
Lc 20ab, by Shimadzu (1 mentions)
Bp460 490, by Olympus (1 mentions)
Hq 535 50m, by Chroma Technology (1 mentions)
Ix70 inverted system microscope, by Olympus (1 mentions)
7100 ce system, by Agilent Technologies (1 mentions)
8 protocols using lc 20ad nano pump
1
Quantifying Genomic 5-mdC by cHILIC-ESI-MS/MS
2
Quantifying Plant Hormone Levels
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Plant hormones of free SA and JA productions were extracted according to a previously described method [63 (link), 64 (link)]. SA and JA were extracted and quantified according to the method of Liu et al. with appropriate modifications [65 (link)]. Briefly, twig samples (0.5 g for each sample) were immediately frozen in liquid nitrogen and ground with pestle and mortar. The ground samples were extracted with 500 μL modified Bieleski solvent (methanol/H2O, 80/20, v/v) at 4 °C for 12 h. The solutions of SA and JA were prepared as internal standards at a concentration of 1 μg/mL in 100% methanol. All nano-LC experiments were performed on a Shimadzu Prominence nano-flow liquid chromatography system (Kyoto, Japan) with two LC-20 AD nano pumps, two vacuum degassers, a LC-20AB HPLC pump, a SIL-20 AC HT autosampler, and a FCV nano valve. The analytical column of poly (MAA-co-EDMA) monolithic column (100 μm i.d., 360 μm o.d., 30-cm long, purchased from Weltech Co., Ltd., Wuhan, China) was connected to the nano-LC system and conditioned with the mobile phase (ACN/H2O, 50/50, v/v) at a flow rate of 600 μL/min for 30 min.
3
Extraction and Quantification of Plant Hormones
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Plant hormones of free SA and JA productions were extracted according to a previously described method [65, 66] . SA and JA were extracted and quanti ed according to the method of Liu et al. with appropriate modi cations [67] . Brie y, twig samples (0.5 g for each sample) were immediately frozen in liquid nitrogen and ground with pestle and mortar. The ground samples were extracted with 500 μL modi ed Bieleski solvent (methanol/H 2 O, 80/20, v/v) at 4 °C for 12 h. The solutions of SA and JA were prepared as internal standards at a concentration of 1 μg/mL in 100% methanol. All nano-LC experiments were performed on a Shimadzu Prominence nano-ow liquid chromatography system (Kyoto, Japan) with two LC-20AD nano pumps, two vacuum degassers, a LC-20AB HPLC pump, a SIL-20AC HT autosampler, and a FCV nano valve. The analytical column of poly (MAA-co-EDMA) monolithic column (100 μm i.d., 360 μm o.d., 30-cm long, purchased from Weltech Co., Ltd., Wuhan, China) was connected to the nano-LC system and conditioned with the mobile phase (ACN/H 2 O, 50/50, v/v) at a ow rate of 600 μL/min for 30 min.
4
Extraction and Quantification of Plant Hormones
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Plant hormones of free SA and JA productions were extracted according to a previously described method [65, 66] . SA and JA were extracted and quanti ed according to the method of Liu et al. with appropriate modi cations [67] . Brie y, twig samples (0.5 g for each sample) were immediately frozen in liquid nitrogen and ground with pestle and mortar. The ground samples were extracted with 500 μL modi ed Bieleski solvent (methanol/H 2 O, 80/20, v/v) at 4 °C for 12 h. The solutions of SA and JA were prepared as internal standards at a concentration of 1 μg/mL in 100% methanol. All nano-LC experiments were performed on a Shimadzu Prominence nano-ow liquid chromatography system (Kyoto, Japan) with two LC-20AD nano pumps, two vacuum degassers, a LC-20AB HPLC pump, a SIL-20AC HT autosampler, and a FCV nano valve. The analytical column of poly (MAA-co-EDMA) monolithic column (100 μm i.d., 360 μm o.d., 30-cm long, purchased from Weltech Co., Ltd., Wuhan, China) was connected to the nano-LC system and conditioned with the mobile phase (ACN/H 2 O, 50/50, v/v) at a ow rate of 600 μL/min for 30 min.
5
Quantification of Plant Hormones SA and JA
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Plant hormones of free SA and JA productions were extracted according to a previously described method [62, 63] . SA and JA were extracted and quanti ed according to the method of Liu et al. with appropriate modi cations [64] . Brie y, twig samples (0.5 g for each sample) were immediately frozen in liquid nitrogen and ground with pestle and mortar. The ground samples were extracted with 500 µL modi ed Bieleski solvent (methanol/H 2 O, 80/20, v/v) at 4 °C for 12 h. The solutions of SA and JA were prepared as internal standards at a concentration of 1 µg/mL in 100% methanol. All nano-LC experiments were performed on a Shimadzu Prominence nano-ow liquid chromatography system (Kyoto, Japan) with two LC-20AD nano pumps, two vacuum degassers, a LC-20AB HPLC pump, a SIL-20AC HT autosampler, and a FCV nano valve. The analytical column of poly (MAA-co-EDMA) monolithic column (100 µm i.d., 360 µm o.d., 30-cm long, purchased from Weltech Co., Ltd., Wuhan, China) was connected to the nano-LC system and conditioned with the mobile phase (ACN/H 2 O, 50/50, v/v) at a ow rate of 600 µL/min for 30 min.
6
Fluorescence Imaging of Coumarin and Amino Acids
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The mobile phase for coumarin analysis was water/acetonitrile (65:35, v/v) at a flow rate of 1 µL/min (LC-20AD nano pump, Shimadzu, Kyoto, Japan). In the case of amino acid analysis, we used water/acetonitrile/TFA (90:10:0.02, v/v/v) as the mobile phase. The flow path was switched using a high-pressure six-way valve (FCV-20H, Shimadzu). Fluorescence excitation was performed using a metal halide lamp. The filter cube was composed of an excitation filter (BP460–490, Olympus, Tokyo, Japan), a dichroic mirror (505DRLP, Omega Optical, Brattleboro, VT, USA), and an emission filter (HQ 535/50m, Chroma Technology, Rockingham, VT, USA). An IX70 inverted microscope system (Olympus, Tokyo, Japan) and an electron-multiplying charge-coupled device camera (iXon3, Andor Technologies, South Windsor, CT, USA) were used to observe the fluorescence images. Detection was performed near the outlet of the pillar array column.
7
Characterization of Monolithic Columns
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All CEC experiments were performed on an Agilent 7100 CE system (Waldbronn, Germany) equipped with a temperature controlled column compartment, a diode array detector and an auto-sampler. The data acquisition was carried out on a chromatographic workstation (Chemistry Station, USA). The Fourier transform infrared spectra (FT-IR) were obtained from a Thermo Nexus 470 FT-IR system (MA, USA). The morphology characterization of the monoliths was performed by a Carl Zeiss Ultra PlusField Emission scanning electron microscope (FESEM, Carl Zeiss, Germany) at an accelerating voltage of 5.0 kV. A Shimadzu LC-20AD NANO pump (Japan) was used to measure the backpressure value of monolithic columns using methanol as the running buffer at the rate of 0.5 μL min -1 .
8
Characterization of Itaconic Acid-based Monolithic Columns
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FTIR was performed
on a Thermo Nexus 470 FTIR system (MA, USA) to obtain the infrared
spectra data. The cross-sectional morphology of the prepared monolithic
columns was examined by a Carl Zeiss Ultra Plus field emission scanning
electron microscope (FESEM, Carl Zeiss, Germany) with an accelerating
voltage of 5.0 kV. Ultrapure water used in the experiment was produced
through a Milli-Q CLX system (Merck, Germany). The backpressure of
the obtained itaconic acid-based monolithic columns was measured on
an LC-20AD NANO pump (Shimadzu, Japan). All HI-CEC separations were
implemented on an Agilent 7100 CE system with a diode array detector
(Waldbronn, Germany). Data collection and processing were carried
out using a chromatographic workstation (Chemistry Station).
on a Thermo Nexus 470 FTIR system (MA, USA) to obtain the infrared
spectra data. The cross-sectional morphology of the prepared monolithic
columns was examined by a Carl Zeiss Ultra Plus field emission scanning
electron microscope (FESEM, Carl Zeiss, Germany) with an accelerating
voltage of 5.0 kV. Ultrapure water used in the experiment was produced
through a Milli-Q CLX system (Merck, Germany). The backpressure of
the obtained itaconic acid-based monolithic columns was measured on
an LC-20AD NANO pump (Shimadzu, Japan). All HI-CEC separations were
implemented on an Agilent 7100 CE system with a diode array detector
(Waldbronn, Germany). Data collection and processing were carried
out using a chromatographic workstation (Chemistry Station).
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