Total RNA from the 2000 pyramidal neurons from each subject and each lamina was purified separately with the QuickGene RNA Tissue kit SII in conjunction with the QuickGene Mini-80 semi-automated station (FujiFilm Life Sciences Division). RNA samples were processed by Genome Explorations Inc., Memphis, TN – a certified Affymetrix® microarray service provider – for microarray assays. Immediately prior to cDNA synthesis, the purity and concentration of RNA samples was determined by readings of OD260/280 using a dual beam UV spectrophotometer and RNA integrity was determined by capillary electrophoresis using the RNA 6000 Nano Lab-on-a-Chip kit and the Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA) following the manufacturers instructions. The purified RNA was amplified with the WT-Ovation™ Pico RNA Amplification System (NuGEN Technologies, Inc). 5–20 ng RNA from each sample was reverse transcribed to complementary DNA (cDNA), amplified, fragmented, and labeled with biotin using the Ovation Pico WTA, Ovation Exon Module, and Encore Biotin Module kits according to the manufacturer’s instructions (NuGEN, San Carlos CA).
Ovation exon module
Manufactured by Tecan
The Ovation Exon Module is a laboratory equipment designed for the amplification and detection of specific DNA sequences. It provides a platform for the analysis of gene expression patterns and the identification of genetic variations. The core function of the Ovation Exon Module is to enable researchers to perform precise and efficient exon-level analysis of gene expression.
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Lab products found in correlation
3 protocols using ovation exon module
1
Microarray Analysis of Pyramidal Neurons
2
Microarray Analysis of Pyramidal Neurons
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Total RNA from the 2000 pyramidal neurons from each subject and each lamina was purified separately with the QuickGene RNA Tissue kit SII in conjunction with the QuickGene Mini-80 semi-automated station (FujiFilm Life Sciences Division). RNA samples were processed by Genome Explorations Inc., Memphis, TN – a certified Affymetrix® microarray service provider – for microarray assays. Immediately prior to cDNA synthesis, the purity and concentration of RNA samples was determined by readings of OD260/280 using a dual beam UV spectrophotometer and RNA integrity was determined by capillary electrophoresis using the RNA 6000 Nano Lab-on-a-Chip kit and the Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA) following the manufacturers instructions. The purified RNA was amplified with the WT-Ovation™ Pico RNA Amplification System (NuGEN Technologies, Inc). 5–20 ng RNA from each sample was reverse transcribed to complementary DNA (cDNA), amplified, fragmented, and labeled with biotin using the Ovation Pico WTA, Ovation Exon Module, and Encore Biotin Module kits according to the manufacturer’s instructions (NuGEN, San Carlos CA).
3
Transcriptome Analysis of Pyramidal Neurons
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Total unpooled RNA from the 2000 pyramidal neurons from each subject was purified with the QuickGene RNA Tissue kit SII in conjunction with the QuickGene Mini-80 semi-automated station (FujiFilm Life Sciences Division). RNA samples were processed by Genome Explorations Inc., Memphis, TNa certified Affymetrix® microarray service providerfor microarray assays.
Immediately prior to cDNA synthesis, the purity and concentration of RNA samples were determined by readings of OD260/280 using a dual beam UV spectrophotometer and RNA integrity was determined by capillary electrophoresis using the RNA 6000 Nano Lab-on-a-Chip kit and the Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA) following the manufacturers instructions. The purified RNA was amplified with the WT-Ovation™ Pico RNA Amplification System (NuGEN Technologies, Inc). 5-20 ng RNA from each sample was reverse transcribed to complementary DNA (cDNA), amplified, fragmented, and labeled with biotin using the Ovation Pico WTA, Ovation Exon Module, and Encore Biotin Module kits according to the manufacturer's instructions (NuGEN, San Carlos CA).
Immediately prior to cDNA synthesis, the purity and concentration of RNA samples were determined by readings of OD260/280 using a dual beam UV spectrophotometer and RNA integrity was determined by capillary electrophoresis using the RNA 6000 Nano Lab-on-a-Chip kit and the Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA) following the manufacturers instructions. The purified RNA was amplified with the WT-Ovation™ Pico RNA Amplification System (NuGEN Technologies, Inc). 5-20 ng RNA from each sample was reverse transcribed to complementary DNA (cDNA), amplified, fragmented, and labeled with biotin using the Ovation Pico WTA, Ovation Exon Module, and Encore Biotin Module kits according to the manufacturer's instructions (NuGEN, San Carlos CA).
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