Ultrapure dnase rnase free distilled water

Thiol-modified DNA probe (5′-thiol-T₂₀-3′) was synthesized and purified by high-performance liquid chromatography (HPLC) by Integrated DNA Technologies (Coralville, USA). Alkaline phosphatase (ALP), sodium pyrophosphate (PPi), copper chloride (CuCl₂), tris(hydroxymethyl)aminomethane, potassium chloride (KCl), sodium chloride (NaCl), ascorbic acid, 3-morpholinopropane-1-sulfonic acid (MOPS), potassium ferricyanide (K₃[Fe(CN)₆]), potassium ferrocyanide (K₄[Fe(CN)₆]), lysozyme, albumin, avidin, glucose oxidase, adenosine 5′-triphosphate (ATP), creatinine, cysteine, glucose and human serum were purchased from Sigma-Aldrich (St. Louis, USA). Ultrapure DNase/RNase-free distilled water (DW) was purchased from Bioneer® (Daejeon, Korea).

All oligonucleotides used in this study were synthesized and purified using PAGE by Bioneer (Daejeon, South Korea), except for the trigger, which was purified by Bio-RP (Bioneer, Daejeon, South Korea). The oligonucleotide sequences are listed in Table S1. The Klenow fragment (3′ → 5′ exo-), 10X NEBuffer 2 (100 mM Tris–HCl, pH 7.9, 500 mM NaCl, 100 mM MgCl₂, and 10 mM DTT), and deoxynucleotide solution mixture (dNTPs) were purchased from New England Biolabs, Inc. (Beverly, MA, USA). SYBR Green I (10,000X) was purchased from Invitrogen (Carlsbad, CA, USA). Leptospira interrogans genomic DNA was purchased from ATCC (Manassas, VA, USA). Human serum was purchased from Sigma-Aldrich (St. Louis, MO, USA). Ultrapure DNase/RNase-free distilled water (DW) was purchased from Bioneer (Daejeon, South Korea) and used in all experiments. All other chemicals were of analytical grade and used without further purification.