Log In Sign up
The largest database of trusted experimental protocols

Anti top2a

Manufactured by Cell Signaling Technology
Visit Supplier
Sourced in United States

Anti-TOP2A is a primary antibody that recognizes the topoisomerase II alpha (TOP2A) protein. TOP2A is an enzyme involved in the regulation of DNA topology during cellular processes such as replication and transcription.

Automatically generated - may contain errors

Lab products found in correlation

Anti γh2ax, by Cell Signaling Technology (2 mentions) Anti akt, by Cell Signaling Technology (1 mentions) Anti cdk4, by Cell Signaling Technology (1 mentions) Anti phospho erk1 2 thr202 tyr204, by Cell Signaling Technology (1 mentions) Anti gfap, by GeneTex (1 mentions) Anti erk1 2, by Cell Signaling Technology (1 mentions) Anti phospho akt ser473, by Cell Signaling Technology (1 mentions) Anti ccnd1, by Cell Signaling Technology (1 mentions) Anti cdk2, by GeneTex (1 mentions) Anti rad51, by GeneTex (1 mentions) Anti ki67, by Cell Signaling Technology (1 mentions) Anti phospho rb, by Cell Signaling Technology (1 mentions) Anti gapdh, by GeneTex (1 mentions) Anti mouse igg hrp, by GE Healthcare (1 mentions) Anti ha peroxidase, by Merck Group (1 mentions) Anti gapdh, by Abcam (1 mentions) Anti α tubulin, by Abcam (1 mentions) Anti rabbit igg hrp, by GE Healthcare (1 mentions) Anti cdc20, by Santa Cruz Biotechnology (1 mentions) Anti vinculin, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

TOP2A (6 citations)

4 protocols using anti top2a

1

Immunoblotting and Immunohistochemical Analysis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunoblotting was performed using anti-phospho-RB (Cell Signaling, Danvers, MA, USA), anti-RB (GeneTex, Irvine, CA, USA), anti-phospho-Akt(Ser473) (Cell Signaling), anti-Akt (Cell Signaling), anti-phospho-Erk1/2(Thr202/Tyr204) (Cell Signaling), anti-Erk1/2 (Cell Signaling), anti-TOP2A (Cell Signaling), anti-CCND1 (Cell Signaling), anti-γH2AX (Cell Signaling), anti-CDK2 (GeneTex), anti-RAD51 (GeneTex), anti-CDK4 (Cell Signaling), and anti-GAPDH (GeneTex) antibodies followed by horseradish peroxidase-conjugated secondary antibodies and visualization using an enhanced chemiluminescence detection system. Immunohistochemical (IHC) sample preparation and staining with anti-GFAP (Genetex), anti-phospho-RB (Cell Signaling), anti-Ki-67 (Cell Signaling), and anti-γH2AX (Cell Signaling) antibodies were carried out as previously described [48 (link)]. Uncropped Western blot images are provided in Figure S5.
Liang M.L., Chen C.H., Liu Y.R., Huang M.H., Lin Y.C., Wong T.T., Lin S.E., Chu S.S., Ding Y.H, & Hsieh T.H. (2020). Abemaciclib, A Selective CDK4/6 Inhibitor, Restricts the Growth of Pediatric Ependymomas. Cancers, 12(12), 3597.
+ Open protocol
+ Expand
2

Comprehensive Immunoblotting Antibody Panel

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The following commercially available primary antibodies were used for immunoblotting: anti-IRS2 (Cell Signaling Technologies, 4502) 1:750; anti-Cdh1/Fzr1 (Sigma Aldrich, CC43) 1:500; anti-anillin (a gift from Christine Field (21 (link))) 1:1000; anti-Aurora B (Bethyl, A300-431) 1:1000; anti-Eg5 (Cell Signaling Technologies, 4203) 1:1000; anti-Top2A (Cell Signaling Technologies, 12286) 1:1000; anti-TK1 (Cell Signaling Technologies, 8960) 1:1000; anti-Mps1 (Abcam, ab11108), 1:1000); anti-APC3 (BD Transduction Laboratories, 610455) 1:500; anti-cyclin B1 (Santa Cruz Biotechnology, sc-752) 1:500; anti-Cdc20 (Santa Cruz Biotechnology, sc-8358) 1:500; anti-c-Myc (9E10, Santa Cruz Biotechnology, sc-40) 1:1000; anti-HA-peroxidase (Sigma Aldrich), 1:1500; anti-cyclin A2 (Santa Cruz Biotechnology, sc-596) 1:500; anti-IRS1 (Cell Signaling Technologies, 2382) 1:750; anti-MyoD1 (Cell Signaling Technologies, 13812) 1:750; anti-GAPDH (Abcam, ab8245) 1:2000; anti-α tubulin (Abcam, ab7291 and Santa Cruz Biotechnology, sc-8035) 1:1000 for both; anti-vinculin (Santa Cruz Biotechnology, sc-73614) 1:2000. Secondary antibodies used: anti-rabbit IgG-HRP (GE Healthcare, NA934) and anti-mouse IgG-HRP (GE Healthcare, NA931V), both at 1:3000 dilutions.
Manohar S., Yu Q., Gygi S.P, & King R.W. (2020). The Insulin Receptor Adaptor IRS2 is an APC/C Substrate That Promotes Cell Cycle Protein Expression and a Robust Spindle Assembly Checkpoint. Molecular & Cellular Proteomics : MCP, 19(9), 1450-1467.
+ Open protocol
+ Expand
3

Western Blot Analysis of Cell Signaling Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were harvested and lysed in 2x SDS-PAGE sample buffer and boiled for 5 minutes. The samples were separated on 4–20% TGX gels (Biorad), transferred to PVDF membranes, and probed with primary and horseradish peroxidase-conjugated secondary antibodies. Primary antibodies purchased from Cell Signaling Technology were: anti-phospho-CDC2/CDK1-Y15 (Cat# 9111), anti-CDC2/CDK1(Cat# 9112), anti-Phospho-PP1α-T320 (Cat# 2581), anti-PP1α (Cat# 2582), anti-γH2AX (Cat# 2595), anti-Cyclin B1 (Cat# 12231), anti-TOP2A (Cat# 12286), anti-CDC20 (Cat# 4823), Aurora kinase sampler kit (Cat# 3875) and phosphor-Ser/Thr kinase substrate antibody sampler kit (Cat# 9920). Rabbit anti-β-actin polyclonal antibody (Cat# A5441), anti-Flag (Cat# F7425), anti-NUSAP1 (Cat# SAB4502109) and mouse anti-Myc antibodies (Cat# M4439) were obtained from Sigma Aldrich. Rat anti-hemagglutinin (HA) high-affinity antibody (Cat# 11-867-431-001) was obtained from Roche. Mouse anti-V5 and anti-V5-HRP antibodies (Cat# R960-25 and R961-25) were obtained from Invitrogen. Anti-phospho-Ser/Thr-Pro MPM2 antibody (Cat# 05–368) was purchased from Millipore. Normal rat IgG (Cat# sc-2026) and mouse IgG (Cat# sc-2025) were obtained from Santa Cruz. Mouse anti-BGLF4 antibody has been described previously [118 (link)].
Li R., Liao G., Nirujogi R.S., Pinto S.M., Shaw P.G., Huang T.C., Wan J., Qian J., Gowda H., Wu X., Lv D.W., Zhang K., Manda S.S., Pandey A, & Hayward S.D. (2015). Phosphoproteomic Profiling Reveals Epstein-Barr Virus Protein Kinase Integration of DNA Damage Response and Mitotic Signaling. PLoS Pathogens, 11(12), e1005346.
+ Open protocol
+ Expand
4

Protein Extraction and Analysis in Zebrafish

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells (0 dpf mRNA overexpression samples) or larvae (3 dpf Top2 inhibitor–treated larvae) were lysed in radioimmunoprecipitation assay buffer (Santa Cruz Biotechnology) supplemented with a cOmplete Mini EDTA-free protease inhibitor tablet (Roche), 5 mM sodium orthovanadate (New England Biolabs), and 10 mM sodium fluoride (Sigma-Aldrich) for 50 min on ice with vortexing every 10 min. The resulting lysate was centrifuged at 14,000g for 15 min at 4°C. The entire volume of the supernatant was saved and subsequently combined with 5× Laemmli buffer before gel loading for Western blot analysis. Protein concentration was measured by Bradford (Bio-Rad) assay. Anti-TOP2A (Cell Signaling Technology, D10G9), anti-H3K27me3 (Cell Signaling Technology, C36B11), and anti–β-actin (Cell Signaling Technology, D6A8) antibodies were used for detection. Signal was detected by enhanced chemiluminescence.
Geng Y., Zhang T., Alonzo I.G., Godar S.C., Yates C., Pluimer B.R., Harrison D.L., Nath A.K., Yeh J.R., Drummond I.A., Bortolato M, & Peterson R.T. (2022). Top2a promotes the development of social behavior via PRC2 and H3K27me3. Science Advances, 8(47), eabm7069.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!