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Mcp 3

Manufactured by Thermo Fisher Scientific
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Sourced in United States

The MCP-3 is a laboratory instrument designed for the detection and quantification of various analytes. It utilizes a multi-channel photometer technology to perform spectrophotometric measurements. The core function of the MCP-3 is to provide accurate and reliable results for a wide range of assays and applications in the scientific research and clinical laboratory settings.

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Lab products found in correlation

Interleukin 6 (il 6), by Thermo Fisher Scientific (6 mentions) Gm csf, by Thermo Fisher Scientific (6 mentions) Cxcl1, by Thermo Fisher Scientific (5 mentions) Cxcl5, by Thermo Fisher Scientific (3 mentions) Cytokine array c3, by RayBiotech (3 mentions) Mcp 3, by R&D Systems (3 mentions) Gm csf, by R&D Systems (3 mentions) Mip 2, by R&D Systems (1 mentions) Cx3cl1, by R&D Systems (1 mentions) Mip 3β, by R&D Systems (1 mentions) Cxcl12, by R&D Systems (1 mentions) Il 17, by R&D Systems (1 mentions) Cxcl10, by R&D Systems (1 mentions) Il 1β, by R&D Systems (1 mentions) Tnf α, by R&D Systems (1 mentions) Interleukin 6 (il 6), by R&D Systems (1 mentions) Mcp 1, by R&D Systems (1 mentions) Interleukin 2 (il 2), by R&D Systems (1 mentions) Il 10, by R&D Systems (1 mentions) Il 18, by R&D Systems (1 mentions)

4 protocols using mcp 3

1

Cytokine and Chemokine Detection in Mice

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The cytokines and chemokines of the mice were detected using corresponding commercial ELISA kits. TNF-α (Cat#: MTA00B), IL-1β (Cat#: MLB00C), IL-6 (Cat#: M6000B), IL-18 (Cat#: DY122-05), MCP-1 (Cat#: SMJE00B), CCL1 (Cat#: DY845), IL-17 (Cat#: SM1700), IL-2 (Cat#: SM2000), IL-10 (Cat#: SM1000B), IL-5 (Cat#: SM5000), MIP-2 (Cat#: SMM200), MIP-3β (Cat#: DY440), MDC (Cat#: MCC220), CX3CL1 (Cat#: MCX310), CXCL10 (Cat#: DY466-05), and CXCL12 (Cat#: DY460) ELISA kits were purchased from R&D Systems (Shanghai, China) and used according to the product specifications. An MCP-3 (Cat#: BMS6006INST) ELISA kit was acquired from Invitrogen. A GCP-2 (Cat#: ab100719) kit was obtained from Abcam. An HMGB1 ELISA kit (Cat#: 326054329) was purchased from Shino-Test Corporation (Kanagawa, Japan). A procalcitonin ELISA kit (Cat#: EF014005) was obtained from Life Sciences. All the corresponding serum was carefully stored at −80 °C in a refrigerator until it was used.
Xu M., Ge C., Qin Y., Lou D., Li Q., Feng J., Wu Y., Hu L., Wang B, & Tan J. (2020). Functional loss of inactive rhomboid-like protein 2 mitigates obesity by suppressing pro-inflammatory macrophage activation-triggered adipose inflammation. Molecular Metabolism, 34, 112-123.
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2

Cytokine Profiling and eMSC Response

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Cytokine Array C3 (RayBiotech Inc., Norcross, GA, USA) was used to determine the cytokines in coculture experiments. The signal intensities of the cytokines were quantified using the Image J software (NIH Image, National Institutes of Health, USA). A fold change ≥ 3 after coculture was considered as potential cytokine candidate. The chemokine (C-X-C motif) ligand 1 (CXCL1), CXCL5, granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-6 (IL-6), monocyte chemoattractant protein 3 (MCP-3) levels serum-free CM collected from coculture experiment of eMSCs with endometrial epithelial and stromal cells from the menstrual phase were determined using enzyme-linked immunosorbent assays (ELISA; IL-6, Invitrogen; CXCL1, CXCL5, GM-CSF and MCP-3, R&D Systems). Four candidate cytokines were shortlisted, and recombinant CXCL1 (1000 pg/ml; PeproTech, Rocky Hill, NJ, USA), CXCL5 (600 pg/ml; PeproTech), GM-CSF (500 pg/ml, PeproTech) and IL-6 (500 pg/ml, PeproTech) at concentrations found in the coculture condition was added to the growth medium of the eMSCs seeded at clonal density (500 cells/cm2) for 15 days.
Xu S., Chan R.W., Li T., Ng E.H, & Yeung W.S. (2020). Understanding the regulatory mechanisms of endometrial cells on activities of endometrial mesenchymal stem-like cells during menstruation. Stem Cell Research & Therapy, 11, 239.
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3

Cytokine Array Analysis of eMSCs

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Cytokine Array C3 (RayBiotech Inc., Norcross, GA, USA) was used to determine the cytokines in coculture experiments. The signal intensities of the cytokines were quanti ed using the Image J software (NIH Image, National Institutes of Health, USA). A fold change ≥ 3 after coculture was considered as potential cytokine candidate. The chemokine (C-X-C motif) ligand 1 (CXCL1), CXCL5, Granulocytemacrophage colony stimulating factor (GM-CSF), interleukin-6 (IL-6), monocyte chemoattractant protein 3 (MCP-3) levels serum free CM collected from coculture experiment of eMSCs with endometrial epithelial and stromal cells from the menstrual phase were determined using enzyme-linked immunosorbent assays (ELISA; IL-6, Invitrogen; CXCL1, CXCL5, GM-CSF and MCP-3, R&D Systems) Four candidate cytokine were shortlisted and recombinant CXCL1 (1000 pg/ml; PeproTech, Rocky Hill, NJ, USA), CXCL5 (600 pg/ml; PeproTech), GM-CSF (500 pg/ml, PeproTech) and IL-6 (500 pg/ml, PeproTech) at concentrations found in the coculture condition was added to the growth medium of the eMSCs seeded at clonal density (500 cell/cm 2 ) for 15 days.
Xu S., Chan R., Li T., Ng E.H.Y., & Yeung W.S. (2020). Understanding the Regulatory Mechanisms of Endometrial Cells on Activities of Endometrial Mesenchymal Stem-Like Cells During Menstruation.
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4

Cytokine Modulation of eMSC Clonal Growth

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cytokine Array C3 (RayBiotech Inc., Norcross, GA, USA) was used to determine the cytokines in coculture experiments. The signal intensities of the cytokines were quanti ed using the Image J software (NIH Image, National Insistutes of Health, USA). A fold change ≥ 3 after coculture was considered as potential cytokine candidate. The chemokine (C-X-C motif) ligand 1 (CXCL1), CXCL5, Granulocytemacrophage colony stimulating factor (GM-CSF), interleukin-6 (IL-6), monocyte chemoattractant protein 3 (MCP-3) levels serum free CM collected from coculture experiment of eMSCs with endometrial epithelial and stromal cells from the menstrual phase were determined using enzyme-linked immunosorbent assays (ELISA; IL-6, Invitrogen; CXCL1, CXCL5, GM-CSF and MCP-3, R&D Systems) Four candidate cytokine were shortlisted and recombinant CXCL1 (1000 pg/ml; PeproTech, Rocky Hill, NJ, USA), CXCL5 (600 pg/ml; PeproTech), GM-CSF (500 pg/ml, PeproTech) and IL-6 (500 pg/ml, PeproTech) at concentrations found in the coculture condition was added to the growth medium of the eMSCs seeded at clonal density (500 cell/cm 2 ) for 15 days.
Xu S., Chan R., Li T., Ng E.H.Y., & Yeung W.S. (2020). Understanding the Regulatory Mechanisms of Endometrial Cells on Activities of Endometrial Mesenchymal Stem-Like Cells During Menstruation.
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