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Human tgf β2 quantikine elisa kits

Manufactured by R&D Systems
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Sourced in United States

The Human TGF-β2 Quantikine ELISA Kits are a quantitative sandwich enzyme immunoassay designed to measure human transforming growth factor beta 2 (TGF-β2) levels in cell culture supernates, serum, and plasma. The assay employs a specific antibody pre-coated onto a microplate.

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Lab products found in correlation

Microplate reader, by Bio-Rad (2 mentions) Human tgf β1, by R&D Systems (1 mentions)

Close spelling variants of this product

Quantikine ELISA human kits Quantikine ELISA kit TGF-β2 Human Quantikine kits Quantikine kits Quantikine ELISA Quantikine Human Human kits Quantikines ELISAs

3 protocols using human tgf β2 quantikine elisa kits

1

TGF-β Depletion Analysis via ELISA

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CM was incubated with IgG binding to TGF-β1 or/and TGF-β2 for 2 h. To confirm TGF-β depletion the level of cytokines was analyzed using sandwich enzyme-linked immunosorbent assay (ELISA) kits (Human TGF-β1 and Human TGF-β2 Quantikine ELISA Kits, R&D Systems, Minneapolis, MN, USA) according to the manufacturer's instructions. The optical density of each reaction was measured at 450 nm using a microplate reader (Bio-Rad) and corrected against absorption at 570 nm.
Wawro M.E., Chojnacka K., Wieczorek-Szukała K., Sobierajska K, & Niewiarowska J. (2018). Invasive Colon Cancer Cells Induce Transdifferentiation of Endothelium to Cancer-Associated Fibroblasts through Microtubules Enriched in Tubulin-β3. International Journal of Molecular Sciences, 20(1), 53.
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2

Quantification of TGF-β1 and TGF-β2 in Conditioned Medium

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Conditioned medium (CM) was recovered from cells growing for 48 h and centrifuged to eliminate cells. TGF-β1 and TGF-β2 were quantified in the CM medium using sandwich enzyme-linked immunosorbent assay (ELISA) kits (Human TGF-β 1 and Human TGF--β 2 Quantikine ELISA Kits, R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions. The optical density of each reaction was measured at 450 nm using a microplate reader (Bio-Rad, Hercules, CA, USA) and corrected against absorption at 570 nm.
Sobierajska K., Wawro M.E, & Niewiarowska J. (2022). Oxidative Stress Enhances the TGF-β2-RhoA-MRTF-A/B Axis in Cells Entering Endothelial-Mesenchymal Transition. International Journal of Molecular Sciences, 23(4), 2062.
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3

Quantifying sCD14 and TGF-β2 in Amniotic Fluid

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The concentrations of sCD14 and TGF-β2 in the AF of the HM were determined by capture ELISA using Human CD14 Quantikine and Human TGF-β2 Quantikine ELISA Kits, respectively (R&D Systems, Minneapolis, MN), according to the manufacturer's instructions (https: / / resources .rndsystems .com/ pdfs/ datasheets/ dy383 .pdf; https: / / resources .rndsystems .com/ pdfs/ datasheets/ dy302 .pdf).
Irazusta A., Rodríguez-Camejo C., Jorcin S., Puyol A., Fazio L., Arias F., Castro M., Hernández A, & López-Pedemonte T. (2020). High-pressure homogenization and high hydrostatic pressure processing of human milk: Preservation of immunological components for human milk banks. Journal of dairy science, 103(7).
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